One-step preparation of amino-PEG modified poly(methyl methacrylate) microchips for electrophoretic separation of biomolecules

Abstract

A simple method for a chemical surface modification of poly(methyl methacrylate) (PMMA) microchips with amino-poly(ethyleneglycol) (PEG-NH(2)) by nucleophilic addition-elimination reaction was developed to improve the separation efficiency and analytical reproducibility in a microchip electrophoresis (MCE) analysis of biomolecules such as proteins and enantiomers. In our procedure, the PEG chains were robustly immobilized only by introducing an aqueous solution of PEG-NH(2) into the PMMA microchannel. The electroosmotic mobilities on the modified chips remained almost constant during 35 days with 37 runs without any recoating. The PEG-NH(2) modified chip provided a fast, reproducible, efficient MCE separation of proteins with a wide variety of isoelectric points within 15s. Furthermore, the application of the modified chip to affinity electrophoresis using bovine serum albumin gave a good chiral separation of amino acids.

NEXAFS and XPS studies of VUV/O<inf>3</inf> treated aromatic polyurea for high-speed electrophoresis microchips

“…The theoretical plate number of polyurea-coated chip was about 600. It is six times larger than that of uncoated PMMA microchip [27]. The sharp peak was realized by negligible adhesion of BSA.…”

Interfacial Properties of Polyethylene Glycol/Vinyltriethoxysilane (PEG/VTES) Copolymers and their Application to Stain Resistance

“…Additionally, PEG does not cause rejection reactions and has been approved by the US FDA to be used in drug and food products due to its excellent biocompatibility. It is widely used in the pharmaceutical industry, agriculture, food handling, biological and material sciences, and chemical engineering fields [7–11]. …”

Fabrication of anti-protein-fouling poly(ethylene glycol) microfluidic chip electrophoresis by sandwich photolithography

“…19,20 For instance, Kitagawa et al modified PMMA microchannel surface with amino-PEG by nucleophilic addition-elimination reaction to enhance separation performance of the MCE. 21 Similarly, a PEG-based surface modification strategy was developed for PDMS microchips to prevent protein adsorption. 22 In this work, we report a simple method to fabricate anti-protein-fouling MCE using a novel sandwich photolithography of PEG prepolymers.…”