One Step Preparation of Peptide-Coated Gold Nanoparticles with Tunable Size

Abstract

Gold nanoparticles (AuNPs) made from self-assembling peptides have been used in many research fields and attracted a great deal of attention due to their high stability, biocompatibility and functionality. However, existing preparation methods for peptide-coated AuNPs are post-synthesis processes, which are complicated and time consuming. Therefore, a one-step preparation method for peptide-coated AuNPs is proposed here. The AuNPs obtained by this method exhibit good stability. Importantly, peptide-coated AuNPs with precise different sizes can be prepared by this method through pH control of reducing reagent tyrosine in range of 10.0~12.7. Thus, the one-step preparation method proposed here provides a significant tool for the research in different fields concerning NP size, stability and biocompatibility.

Au Nanobead Chains with Tunable Plasmon Resonance and Intense Optical Scattering: Scalable Green Synthesis, Monte Carlo Assembly Kinetics, Discrete Dipole Approximation Modeling, and Nano-Biophotonic Application

“…Furthermore, in terms of the pH stability, Jia et al show that CALNN conjugated Au NPs have stable colloidal properties for pH < 10. 76 For cysteamine, within the range of pH 4− 10, Au−cysteamine aggregates have been shown to be stable, with pH > 10 resulting in the deprotonation of the sulfur moiety, leading to destabilization of the product. 55,77 Notably, our in vitro experiments were performed at pH = 7.4, under stable pH conditions.…”

Hydrophobized Short Peptide Amphiphile Functionalized Gold Nanoparticles as Antibacterial Biomaterials

“…Self‐assembling short peptide conjugates have extensively been used in the production of hierarchical nanomaterial because of their versatility [28–30] . These organized materials when conjugated with bioactive metals, enhances the biocompatibility and cooperativity along with electromagnetic and optical properties of nanoparticles [29–32] . The coating of gold nanoparticles by designed peptide conjugates modulates the surface properties with specific biological function and reduces its side effects or toxicity [31–33] …”

“…[28][29][30] These organized materials when conjugated with bioactive metals, enhances the biocompatibility and cooperativity along with electromagnetic and optical properties of nanoparticles. [29][30][31][32] The coating of gold nanoparticles by designed peptide conjugates modulates the surface properties with specific biological function and reduces its side effects or toxicity. [31][32][33] Short peptide amphiphiles (sPAs) or Lipid-peptide conjugates exhibits a wide range of tightly packed self-assembling nanostructures such as spherical micelles, vesicles, fibers and ribbons, etc.…”

“…[29][30][31][32] The coating of gold nanoparticles by designed peptide conjugates modulates the surface properties with specific biological function and reduces its side effects or toxicity. [31][32][33] Short peptide amphiphiles (sPAs) or Lipid-peptide conjugates exhibits a wide range of tightly packed self-assembling nanostructures such as spherical micelles, vesicles, fibers and ribbons, etc. [3,4,[34][35][36] These nanostructures demonstrate delicate balance between hydrophobicity from lipid alkyl chain and hydrophilicity from a group of peptide side chains.…”

Active Targeting Gold Nanoparticle for Chemotherapy Drug Delivery: A Review

“…Modifying the peptide sequence can contribute to peptide-AuNP synthesis without other additional linkers. 79 It had been reported that peptide-AuNP conjugates enhance the macrophage responses compared with sole peptide or AuNP. 80 Overexpression receptor αβ3 integrin was detected in the melanoma, glioblastoma and tumor vasculature.…”