One-step purification of lactoperoxidase from bovine milk by affinity chromatography

Carbonic anhydrase inhibitory properties of novel sulfonamide derivatives of aminoindanes and aminotetralins

“…Amines 24-27 were synthesized from their corresponding salts 14, 15, 20 and 21 by the same procedure with yields of 92%, 95%, 95% and 94%, respectively. N-(5,6-dimethoxy-2,3-dihydro-1H-inden-2-yl) Purification of hCA I and hCA II by affinity chromatography CA isoenzymes were purified affinity chromatography using a column packed with Sepharose 4B-L-Tyrosine sulphanilamide resin via a simple one-step method using Sepharose-4B-L tyrosine-sulphanilamide affinity gel chromatography; described previously 27,28 .…”

The effects of some bromophenols on human carbonic anhydrase isoenzymes

“…Affinity chromatography is a purification method of separating biochemical mixtures based on a highly specific interaction such as that between enzyme and substrate 29 . In this study, both CA isoenzymes were purified by Sepharose-4B-L-tyrosine-sulfanilamide affinity chromatography in a single step [30][31][32] .…”

Synthesis of 4,5-disubstituted-2-thioxo-1,2,3,4-tetrahydropyrimidines and investigation of their acetylcholinesterase, butyrylcholinesterase, carbonic anhydrase I/II inhibitory and antioxidant activities

“…After the mixture was stirred for 5 minutes, p-tolualdehyde (1.18 mL, 0.01 mol) was added and it was stirred for 30 min. Then TFAA (0.02 mL) was added to the reaction mixture and it was stirred by heating at [70][71][72][73][74][75] C. The progress of the reaction was controlled by Sulifol UV 254 plate. After determining the full completion of reaction, solution was evaporated and is cleansed in ethyl alcohol solution.…”