One-step quantification of salivary exosomes based on combined aptamer recognition and quantum dot signal amplification

Wu, Min; Chen, Zhuo-Kun; Xie, Qihui; Xiao, Bo-Lin; Zhou, Gang; Chen, Gang; Bian, Zhuan

doi:10.1016/j.bios.2020.112733

Cited by 61 publications

(43 citation statements)

References 48 publications

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“…Monodisperse QDs with various functional ligands are readily accessible through commercial resources. Thus, QDs have attracted increasing interests for molecular exosome detection [35][36][37][38][39][40][41][42][43]. For example, Bai et al used QDs to label and detect EXOs of different surface markers after trapping magnetic bead (MB)-isolated EXOs in a microfluidic micropillar chip [39].…”

Section: Introductionmentioning

confidence: 99%

Exosomal Surface Protein Detection with Quantum Dots and Immunomagnetic Capture for Cancer Detection

et al. 2021

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Exosomes carry molecular contents reflective of parental cells and thereby hold great potential as a source of biomarkers for non-invasive cancer detection and monitoring. However, simple and rapid exosomal molecular detection remains challenging. Here, we report a facile method for exosome surface protein detection using quantum dot coupled with immunomagnetic capture and enrichment. In this method, exosomes were captured by magnetic beads based on CD81 protein expression. Surface protein markers of interest were recognized by primary antibody and then detected by secondary antibody-conjugated quantum dot with fluorescent spectroscopy. Validated by ELISA, our method can specifically detect different surface markers on exosomes from different cancer cell lines and differentiate cancer exosomes from normal exosomes. The clinical potential was demonstrated with pilot plasma samples using HER2-positive breast cancer as the disease model. The results show that exosomes from HER2-positive breast cancer patients exhibited a five times higher level of HER2 expression than healthy controls. Exosomal HER2 showed strong diagnostic power for HER2-positive patients, with the area under the curve of 0.969. This quantum dot-based exosome method is rapid (less than 5 h) and only requires microliters of diluted plasma without pre-purification, practical for routine use for basic vesicle research, and clinical applications.

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Section: Introductionmentioning

confidence: 99%

Exosomal Surface Protein Detection with Quantum Dots and Immunomagnetic Capture for Cancer Detection

et al. 2021

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“…A variety of detection mechanisms have been employed in biosensing, including electrochemical [111][112][113]115,116,118,119,121,122,125,129,133,135,141], photoelectrochemical [114], optical [100,110,117,124,126,127,140], and mass-based systems [131]. All of the above biosensing methods enable detection with high sensitivity, and enhanced specificity [142].…”

Section: Biosensors and Bioelectronic Platforms For The Detection Of Oral Cancer Biomarkers In Salivamentioning

confidence: 99%

Section: Optical Biosensorsmentioning

confidence: 99%

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Malignancies and Biosensors: A Focus on Oral Cancer Detection through Salivary Biomarkers

et al. 2021

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Oral cancer is among the deadliest types of malignancy due to the late stage at which it is usually diagnosed, leaving the patient with an average five-year survival rate of less than 50%. The booming field of biosensing and point of care diagnostics can, in this regard, play a major role in the early detection of oral cancer. Saliva is gaining interest as an alternative biofluid for non-invasive diagnostics, and many salivary biomarkers of oral cancer have been proposed. While these findings are promising for the application of salivaomics tools in routine practice, studies on larger cohorts are still needed for clinical validation. This review aims to summarize the most recent development in the field of biosensing related to the detection of salivary biomarkers commonly associated with oral cancer. An introduction to oral cancer diagnosis, prognosis and treatment is given to define the clinical problem clearly, then saliva as an alternative biofluid is presented, along with its advantages, disadvantages, and collection procedures. Finally, a brief paragraph on the most promising salivary biomarkers introduces the sensing technologies commonly exploited to detect oral cancer markers in saliva. Hence this review provides a comprehensive overview of both the clinical and technological advantages and challenges associated with oral cancer detection through salivary biomarkers.

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“…93,94 Wu et al proposed a "one-step" strategy for the detection of exosomes using aptamers as the biorecognition elements and QDs as the signal-amplified reporters (Figure 2A). 95 CD63 aptamer was anchored on the surface of magnetic microspheres (MMs) and tethered to the selfassembled DNA concatemers. Then, streptavidin (SA)-conjugated QDs were used to label the biotin-modified DNA concatemers.…”

Section: Nanomaterials-based Optical Biosensors For Exosome Detection Fluorescence Biosensorsmentioning

confidence: 99%

Progress in Nanomaterials-Based Optical and Electrochemical Methods for the Assays of Exosomes

Ma¹,

Hao²,

Liu³

2021

IJN

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One-step quantification of salivary exosomes based on combined aptamer recognition and quantum dot signal amplification

Cited by 61 publications

References 48 publications

Exosomal Surface Protein Detection with Quantum Dots and Immunomagnetic Capture for Cancer Detection

Exosomal Surface Protein Detection with Quantum Dots and Immunomagnetic Capture for Cancer Detection

Malignancies and Biosensors: A Focus on Oral Cancer Detection through Salivary Biomarkers

Progress in Nanomaterials-Based Optical and Electrochemical Methods for the Assays of Exosomes

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