“…When THF was used as the solvent for copolymerization on a mixture of a-NCA and b-NTA, using Boc-a-L-Lys NCA and Bn-b 3 -LCHG NTA as the model, we found no significant gradient separation in compositional profile of two amino acid subunits within the poly-a/b-peptide chain, though Boc-a-L-Lys NCA have a slightly higher reactivity than Bn-b 3 -LCHG NTA according to the kinetic plot of residual monomer (Figures 3A-3C) and instantaneous copolymer composition F a-NCA (Figures 3C and S19). (Gleede et al, 2019) This copolymerization proceeded close to ''ideal copolymerization'' (r a-NCA 3 r b-NTA = 0.93, r a-NCA 3 r b-NTA = 1 means ideal copolymerization) (Yasir et al, 2020), which in combination with the instantaneous copolymer composition F a-NCA revealed that the resulting poly-a/b-peptide was a random-like copolymer and the b-amino acid residue has a nearly even distribution along poly-a/b-peptide chain (Figure 3C) (Yasir et al, 2020). Poly-a/b-peptides have proven to be important mimics and modifications of nature polypeptides, such as host defense peptide (HDP), to effectively improve the stability upon enzymatic degradation and therapeutic potential (Konai et al, 2018;Schmitt et al, 2004Schmitt et al, , 2007.…”