One-step sandwich enzyme immunoassay for insulin using monoclonal antibodies

Bürgi, W; Briner, Myriam; Franken, Norbert; Kessler, Asa

doi:10.1016/s0009-9120(88)80087-0

Cited by 124 publications

(54 citation statements)

References 5 publications

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“…Glucose (Trinder 1959), insulin (Burgi et al, 1988), HbA1c Sudhakar & Pattabiraman (1981), total cholesterol (Allain et al, 1974), triglycerides (Fassati & Prencipe 1982), HDL-C (Lopes et al, 1977), LDL-C and VLDL-C were calculated by using the method of Friedewald et al, (1972). Uric acid, urea nitrogen and creatinine were estimated according to the methods described by Fossati et al, (1980), Patton & Crouch (1977) and Bartels et al, (1972) respectively.…”

Section: Biochemical Analysis Of Serummentioning

confidence: 99%

Potential Effect of Date Pits Fortified Bread on Diabetic Rats

Halaby¹

2014

IJNFS

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Abstract:The present work was conducted to evaluate the effect of fortified pan bread with 5%, 10%, and 15% date pits powder on the chemical constituents, the nutritional value, and rheological characteristics of different dough samples using farinograph and on its sensory properties. The rheological properties of dough referred that there was an increase in water absorption in all blends compared to the control sample, and there was an increase in dough development, dough stability and degree of softening at 15% compared with 5% or 10% only. Pan bread fortified with 15% date pits showed the highest score in overall acceptability when compared to control pan bread and other various concentrations of pits. Twenty four adult male albino rats were divided into four groups. The first group (6 rats) fed on basal diet as a (negative control group). The second main group (6 rats) was injected with alloxan to induce hyperglycemia (positive control group) and then the rats fed on the same basal diet. The other 12 rats after being injected with alloxan were divided into 2 groups (3 & 4) and received basal diets containing fortified bread with 10% and 15% date pits powder for 45 days respectively. Biological evaluation was carried out by determination of body weight gain and food intake. At the end of the experimental period rats were sacrificed, blood samples were collected from the aorta then separate serum to determine glucose, insulin, HbA1c, total cholesterol and other lipid , and also to determine each of kidney and liver functions of experimental rats. Liver and Pancreas were removed to demonstrate histopathological observation. Our results showed significant reduction in the glucose, HbA1c, TC, TG, LDL-C and VLDL-C, as well as reducing hazards on liver and kidney functions compared with positive control group. Histopathological observation proved that the last group diet (with 15% date pits) looked like the negative control group. So date pits powder fortified bread is recommended to gain nutritional and healthy benefit to decrease the risk of diabetic diseases.

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Section: Biochemical Analysis Of Serummentioning

confidence: 99%

Potential Effect of Date Pits Fortified Bread on Diabetic Rats

Halaby¹

2014

IJNFS

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“…Serum Insulin Level Serum insulin was measured by enzyme linked imunosorbant assay (ELISA) using the kit 28) (Boehringer Mannheim Diagnostic, Mannheim, Germany). The intra assay variation was 4.9%.…”

Section: Chemicalsmentioning

confidence: 99%

Protection of Testicular Dysfunctions by MTEC, a Formulated Herbal Drug, in Streptozotocin Induced Diabetic Rat

Mallick

Mandal

Barik

et al. 2007

Biological & Pharmaceutical Bulletin

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Diabetes is associated with declining sexual function in both male 1) and female 2) individuals. In case of female, diabetic symptoms consistent with autonomic neuropathy associated with decreased subjective sexual arousal. 3) In recent study Erol et al. noted that women with type 2 diabetes experienced higher prevalence rates of sexual dysfunction when compared to non diabetic. 4) Approximately 80% of diabetic patients complained loss of libido, 60% had diminished clitoral sensitivity, 50% experienced organic dysfunction, 40% experienced vaginal discomfort, and 40% experienced vaginal dryness. 5) Type 1 diabetic women experienced higher prevalence of reduced vasocongestion and reduced vaginal lubrication to erotic stimuli. 6) Thus these clinical studies suggest that both type 1 and type 2 impede the sexual arousal responses in women.Male reproductive alterations have been widely reported in model animals and human with diabetes. 7) Streptozotocin induced diabetes in male rats resulted atrophy of sex organ, changes in histoarchitecture of ventral prostate, 8) diminution in sperm count, 8) along with low levels in plasma gonadotrophins 9,10) and testosterone. 11) The copulatory behavior of diabetic rat has been collapsed 12) along with low fertility in induced normal mating. 12) In human, diabetes is associated with erectile dysfunction 13,14) along with loss of libido 1,5) and abnormal morphology of sperm, low plasma levels of gonadotrophins and testosterone. 15) Though there are several reports about dysfunctions of the male reproductive activity in diabetic state but the data are confusing, and the exact role that insulin plays in the regulation of male reproductive function is still unclear.In traditional practice, medicinal plants are used in many countries to control diabetes mellitus. The National Centre for Complementary and Alternative Medicine, established in 1998 by the United States Government where development of herbal medicine is one of the important subjects of study. 16,17) Moreover the herbal drug is of first choice at present because plant drugs are to be less toxic and more free from side effect than synthetic one. 18) M. paradisiaca is a tree like herb belonging to the Musaceae family and different parts of the plant have medicinal value. 19,20) T. indica LINN is tree type of plant belonging to the Caesalpiniaceae family was used as a traditional medicine for the management of diabetes mellitus. 21) E. Jambolana is used as traditional medicine for the management of diabetes. 19) This plant is found all over India and belongs to the Myrtaceae family. Jambu bark cure hemorrhages, burning sensation, dysentery, diarrhea, diabetes, excessive thirst, dyspepsia, cough and asthma and is used in preparation of astringent decoction for gargles and washes. 20) Seeds are used for the treatment of diabetes. 20) Coccinia indica is under Cucurbitaceae family and has medicinal importance as observed earlier by others. 22) In our previous work we have reported the antidiabetic effect of some of these plant pa...

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“…Insulin was assayed in serum using a commercial kit by Enzyme Linked Immune Sorbent Assay (ELISA) technique based on the method of Brugi, et al [20].…”

Section: Determination Of Serum Glucose and Insulinmentioning

confidence: 99%