One‐step Separation and Purification of Three Lignans and One Flavonol from <i>Sinopodophyllum emodi</i> by Medium‐pressure Liquid Chromatography and High‐speed Counter‐current Chromatography

Wang, Ping; Liu, Yongling; Chen, Tao; Xu, Wenhua; You, Jinmao; Liu, Yongjun; Li, Yulin

doi:10.1002/pca.2438

Cited by 13 publications

(3 citation statements)

References 14 publications

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“…Cao et al (2010) reported the purification of four flavonids and nine biflavonids from S. doederleinii by traditional phytochemical tools. The conventional separation and purification methods required a multi-step protocol including solvent extractions, repeated thin-layer chromatography (TLC) and silica-gel column chromatography (SGC), polyamide column chromatography (PAC) and Sephadex LH20 gel chromatography (LH20 GC), etc., which were tedious, time-consuming and costexpensive, and also resulted in a relatively lower preparative capacity due to irreversible adsorptions of compounds onto the stationary phase materials during separation (Ito, 1981;Wang et al, 2011Wang et al, , 2013Yoon et al, 2011). In addition, the ingredients obtained from the herbal medicine studied are numerous, possibly causing aimlessness and time-consuming screening process for evaluation of their bioactivity.…”

Section: Introductionmentioning

confidence: 99%

Preparative Isolation of six Anti‐Tumour Biflavonoids from Selaginella Doederleinii Hieron by High‐Speed Counter‐Current Chromatography

Zhao

et al. 2013

Phytochemical Analysis

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Section: Introductionmentioning

confidence: 99%

Preparative Isolation of six Anti‐Tumour Biflavonoids from Selaginella Doederleinii Hieron by High‐Speed Counter‐Current Chromatography

Zhao

et al. 2013

Phytochemical Analysis

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“…Flash chromatography on silica gel or Sephadex LH-20 columns has been used for years as the most popular preliminary method for the initial fractionation, and separation, of the samples, as well as for the purification and preparative isolation of natural compounds from raw materials [53]. In addition, various chromatographic methods have been mentioned in the literature, such as open column chromatography [107,108], medium performance liquid chromatography (MPLC) [109], and the most popular method-semipreparative HPLC [110].…”

Section: Isolation and Purificationmentioning

confidence: 99%

Extraction Techniques and Analytical Methods for Isolation and Characterization of Lignans

Patyra

Kołtun-Jasion

Jakubiak

et al. 2022

Plants

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Lignans are a group of natural polyphenols present in medicinal plants and in plants which are a part of the human diet for which more and more pharmacological activities, such as antimicrobial, anti-inflammatory, hypoglycemic, and cytoprotective, are being reported. However, it is their cytotoxic activities that are best understood and which have shed light on this group. Two anticancer drugs, etoposide, and teniposide, were derived from a potent cytotoxic agent—podophyllotoxin from the roots of Podophyllum peltatum. The evidence from clinical and observational studies suggests that human microbiota metabolites (enterolactone, enterodiol) of dietary lignans (secoisolariciresinol, pinoresinol, lariciresinol, matairesinol, syringaresinol, medioresinol, and sesamin) are associated with a reduced risk of some hormone-dependent cancers. The biological in vitro, pharmacological in vivo investigations, and clinical studies demand significant amounts of pure compounds, as well as the use of well-defined and standardized extracts. That is why proper extract preparation, optimization of lignan extraction, and identification are crucial steps in the development of lignan use in medicine. This review focuses on lignan extraction, purification, fractionation, separation, and isolation methods, as well as on chromatographic, spectrometric, and spectroscopic techniques for their qualitative and quantitative analysis.

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“…S. hexandrum embodies abundant pharmacological effects such as anti-proliferative, anti-viral, anti-in ammatory, anti-bacterial, insecticide and cytotoxic activities [2]. In recent decades, the main researches on S. hexandrum have been chie y focused on the establishment of chromatographic methods to quantitatively analyze and measure the content of podophyllotoxin, the chemical compositions and pharmacological effects, and how to prompt the sustainable development and utilization of this endangered plant [3][4][5]. So far, numerous chemical components have been isolated and identi ed from S. hexandrum, namely lignans, avonoids, saponins, polysaccharides and tannins, among which lignans are primarily composed of podophyllotoxin [3,6].…”

Section: Introductionmentioning

confidence: 99%

Potential Anti-Proliferative, Anti-Inflammatory and Anti-Viral Components from Sinopodophyllum Hexandrum Explored Using Bio-Affinity Ultrafiltration with Multiple Drug Targets

Li¹,

Chen²,

Zhang³

et al. 2021

Preprint

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Background: Sinopodophyllum hexandrum (S. hexandrum) is a typical Chinese herbal medicine with numerous components and remarkable pharmacological activities. However, the specific phytochemicals responsible for its anti-proliferative, anti-inflammatory and anti-viral effects remain unexplored.Methods: The integrated analytical strategy combining bio-affinity ultrafiltration with multiple drug targets was developed to rapidly screen and identify bioactive ligands from S. hexandrum. The in vitro anti-proliferative and COX-2 inhibitory assays of bioactive ligands screened were further verified by sulforhodamine B (SRB) cell proliferation and cytotoxicity detection and COX-2 inhibitor screening kits, respectively. Molecular docking analysis was also implemented by the AutoDockTools 1.5.6 software.Results: 10, 7, 9 and 9 phytochemicals were screened out and identified as the potential Topo I, Topo II, COX-2 and ACE2 ligands, respectively. Hereinto, podophyllotoxin and quercetin with higher EF values displayed strong inhibitory effects on A549 and HT-29 cells comparable with etoposide and 5-FU. Furthermore, compared with indomethacin at 0.73 ± 0.07 mM, podophyllotoxin and kaempferol with higher EF values exerted stronger inhibitory effects with IC50 values at 0.36 ± 0.02 mM and 10.49 ± 0.61 mM, respectively. Additionally, the optimal binding sites and mode of action between bioactive ligands and multiple drug targets were determined by molecular docking. Wherein, isorhamnetin showed a stronger affinity to ACE2 with the binding energy of -5.72 kcal/mol and the IC50 value at 63.95 mM, lower than MLN-4760 (-4.27 kcal/mol and 738.62 mM). Conclusions: The integrative strategy combining multiple drug targets and bio-affinity ultrafiltration LC-MS in the present study showed very promising potential for the quick screening and identifying bioactive ligands in S. hexandrum for Topo I, Topo II, COX-2 and ACE2, and some bioactive compounds screened out from this work were verified with other in vitro assays, and even better than those positive drugs of interest. Based on these findings, we then first constructed an interacting network among multi-components and multi-targets. In this way, we showcased a quick and reliable experimental strategy for uncovering the underlying mechanism of the empirical traditional applications of S. hexandrum which could also provide valuable information for better understanding the therapeutic targets and therapeutic ligands of other herbal medicines.

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One‐step Separation and Purification of Three Lignans and One Flavonol from Sinopodophyllum emodi by Medium‐pressure Liquid Chromatography and High‐speed Counter‐current Chromatography

Abstract: Three lignans (4'-demethyl podophyllotoxin, podophyllotoxin, deoxypodophyllotoxin) and one flavonol (kaempferol) were successfully isolated by HSCCC and MPLC in one step.

Cited by 13 publications

References 14 publications

Preparative Isolation of six Anti‐Tumour Biflavonoids from Selaginella Doederleinii Hieron by High‐Speed Counter‐Current Chromatography

Preparative Isolation of six Anti‐Tumour Biflavonoids from Selaginella Doederleinii Hieron by High‐Speed Counter‐Current Chromatography

Extraction Techniques and Analytical Methods for Isolation and Characterization of Lignans

Potential Anti-Proliferative, Anti-Inflammatory and Anti-Viral Components from Sinopodophyllum Hexandrum Explored Using Bio-Affinity Ultrafiltration with Multiple Drug Targets

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