One-Step Solid Extraction for Simultaneous Determination of Eleven Commonly Used Anticancer Drugs and One Active Metabolite in Human Plasma by HPLC-MS/MS

Wang

International Journal of Analytical Chemistry

et al. 2021

Self Cite

Cell is the basic unit of structure and function of all living bodies. The study of intracellular drug concentration distribution is helpful to understand the drug efficacy of target site. Pemetrexed is a new multitarget folate antagonist with pyrrolidine group as its core structure. An ultra high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method was developed for rapid quantification of pemetrexed concentration in human breast cancer cells (MCF-7). Sample pretreatment was completed by protein precipitation using methanol. The optimized chromatographic separation was achieved on a ZORBAX SB-C18 column (2.1 × 150 mm, 3.5 μm). The column was equilibrated with initial mobile phase and eluted under gradient phases containing 0.1% formic acid in water (phase A) and in acetonitrile (phase B). The gradient program started at 5% B, increased to 95% B in 2 min, and then held at 95% B for 1.5 min. The linear range of pemetrexed in cells and nucleus was 2.0–200.0 ng/mL, while in the medium sample it was 50.0–5000.0 ng/ml, and the correlation coefficients were all greater than 0.99. The recovery was 47.50–67.55% (2.0–200.0 ng/mL) and 82.72–97.15% (50.0–5000.0 ng/mL), and the matrix effect was 98.10–100.62% (2.0–200.0 ng/mL) and 89.78–97.65% (50.0–5000.0 ng/mL). Interday precision and intraday precision (RSD%) were less than 15.0% (for LLOQ, less than 20%), and accuracy (RE%) was within ±15%; the deviation of stability was within ±15%, all meeting the requirements of biological sample analysis. The results of intracellular samples showed that the concentration of pemetrexed reached its peak at 3 h after administration. The concentration of pemetrexed in the nucleus continued to increase 2 h after administration and may have reached the maximum concentration at 6 h.

Section: Methodological Studymentioning

confidence: 99%

Rapid Determination of Pemetrexed Concentration and Distribution in Human Breast Cancer Cells (McF-7) Based on UHPLC-MS/MS

Wang

International Journal of Analytical Chemistry

et al. 2021

Self Cite

Biomedical Chromatography

“…Majority of the LC–MS/MS methods (Bai et al, ; Canal‐Raffin et al, ; de Jonge et al, ; DiFrancesco, Griggs, Donnelly, & Robert DiCenzo, ; Ekhart et al, ; Gao et al, ; Shu et al, , ; Zhou et al, ) quantified CP and its metabolites from patient samples used in clinical studies. A simple protein precipitation enabled the quantification of CP and its metabolites by HPLC coupled with electrospray ionization coupled to MS (ESI‐MS/MS) using acetonitrile and methanol mixture as an extraction solvent with an LLOQ of 200 and 50 ng/mL for CP and 4‐OHPC, respectively (de Jonge et al, ; Ekhart et al, ).…”

Section: Case Studiesmentioning

confidence: 99%

“…Majority of the LC-MS/MS methods (Bai et al, 2009;Canal-Raffin et al, 2016;de Jonge et al, 2004;DiFrancesco, Griggs, Donnelly, & Robert DiCenzo, 2007;Ekhart et al, 2007;Gao et al, 2018;Shu et al, 2011Shu et al, , 2016Zhou et al, 2012) The LLOQ was 1 ng/mL for CP or fludarabine (Silvertand et al, 2005).…”

Section: -Ohcp Is Highly Unstable In Biological Matrices and Rapidlymentioning

confidence: 99%

“…A derivatization using dansylhydrazine and hydrochloric acid was employed for 4-OHCP; this method used ethyl acetate for extraction and a C18 column for separation. The SPE method was adopted for the extraction of CP, doxorubicin, and doxorubicinol and CP, paclitaxel, docetaxel, vinblastine, vinorelbine, pemetrexed, carboplatin, etoposide, ifosfamide, gemcitabine, irinotecan, and SN-38 from human plasma samples(Gao et al, 2018).A simultaneous quantification of CP, thalidomide, lenalidomide, bortezomib, dexamethasone, and adriamycin in human serum samples was achieved using LC-MS/MS positive ionization mode(Shu et al, 2016). Various analytes were separated by a gradient elution using a C18 column at a flow rate of less than 0.3 mL/min Zhou et al (2012).…”

mentioning

confidence: 99%

See 1 more Smart Citation

A review of bioanalytical methods for chronic lymphocytic leukemia drugs and metabolites in biological matrices

Suresh

Trivedi

Srinivas³

et al. 2019

Quantitation of drugs used for the treatment of chronic lymphocytic leukemia in various biological matrices during both pre‐clinical and clinical developments is very important, often in routine therapeutic drug monitoring. The first developed methods for quantitation were traditionally done on LC in combination with either UV or fluorescence detection. However, the emergence of LC with mass spectrometry in tandem in early 1990s has revolutionized the quantitation as it has provided better sensitivity and selectivity within a shorter run time; therefore it has become the choice of method for the analysis of various drugs. In this article, an overview of various bioanalytical methods (HPLC or LC–MS/MS) for the quantification of drugs for the treatment of chronic lymphocytic leukemia, along with applicability of these methods, is given.

“…Several methods have been reported in the literature for the estimation of ETO, MOX and NAL in their pharmaceutical dosage form and/or biological fluids; for ETO: spectrophotometric [8], spectrofluorimetric (SF) [9], electrochemical [10,11], high-performance thin-layer chromatography (HPTLC) [12], high-performance liquid chromatography (HPLC) [13][14][15][16][17], gas chromatography (GC) [18] and capillary electrophoresis (CP) [19]; for MOX: spectrophotometric [20,21], SF [22], electrochemical [23], HPTLC [24], HPLC [25][26][27] and CP [28] and for NAL spectrophotometric [29][30][31], SF [31,32], flow injection analysis [33,34], HPLC [35][36][37] and GC [38]. These presented methods did not guide the monitoring of these three drugs in the biological matrices.…”

Section: Introductionmentioning

confidence: 99%

Eco-friendly fluorimetric approaches for the simultaneous estimation of the co-administered ternary mixture: etoposide, moxifloxacin and nalbuphine

2021

Antineoplastic drugs, etoposide (ETO), are widely used in leukaemia. A patient with leukaemia has a relative infection with pneumonia treated by fluoroquinolones as moxifloxacin HCL (MOX). Because opioid analgesic as nalbuphine HCL (NAL) does not have a ceiling dose, it is used to manage the distasteful sensory in leukaemia. Consequently, green methods for synchronous spectrofluorimetric quantification of a ternary mixture of ETO, MOX and NAL were developed. The first approach relies simply on the estimation of MOX at 371 nm by conventional synchronous fluorimetric technique (Δ λ of 60 nm). The second approach depends on applying the first derivative synchronous fluorimetric technique (Δ λ of 60 nm) for simultaneous estimation of ETO and NAL at 257 and 273 nm, respectively. A good linear correlation was obtained in the ranges of 0.04–0.40, 0.10–1.00 and 0.50–5.00 µg ml −1 for MOX, ETO and NAL, respectively. Moreover, the proposed approaches were successfully applied for the estimation of the studied drugs in the pharmaceutical dosage forms. Additionally, the synchronous assessment of ETO, MOX and NAL in the spiked human urine was successfully attained by the facile protein precipitation technique. The mean % recoveries in spiked human urine were 99.49, 98.07 and 98.48 for MOX, ETO and NAL, respectively.