One-step synthesis of gold and silver non-spherical nanoparticles mediated by Eosin Methylene Blue agar

Abstract

Nowadays, there are several approaches reported to accomplish the green synthesis of metal nanoparticles by using bacterial and fungi supernatants or by-products generated by these microorganisms. Therefore, agars as solely reductive regents have started to be used in order to obtain metal nanoparticles. This paper shows the results of the synthesis of gold and silver nanoparticles with different morphology, mainly triangular and truncated triangular, using Eosin Methylene Blue (EMB) agar as reducing agent. To control the reaction process, the necessary activation energy for the reducer was provided by three different techniques: microwave radiation, using a domestic microwave oven, ultraviolet radiation, and heating on a conventional plate. The evolution of the reduction process and stability of the samples was performed by ultraviolet visible spectroscopy. Morphology was carefully analyzed using high-resolution transmission electron microscopy (HRTEM) and Transmission electron microscopy (TEM). A one step synthesis for gold and silver nanoparticles was optimized with an eco-friendly and economic process.

Rowan Berries: A Potential Source for Green Synthesis of Extremely Monodisperse Gold and Silver Nanoparticles and Their Antimicrobial Property

“…The primary observation of nanoparticles formation was supported by visual inspection of the color change in the SM and simultaneous recording of the UV-Vis spectra to check the SPR. UV-Vis techniques provide information about nanoparticles' reduction, shape, size, and morphology [28]. RB-AuNPs showed maximum absorbance in the region of 500-600 nm and RB-AgNPs in 400-500 nm, consistent with the other reports.…”

“…Thus nanoparticles formed in the solution (Figure 1). The optimization studies for RB-AuNPs and RB-AgNPs were also conducted by using UV-Vis spectrum analysis and visible observations [28]. For RB-AuNPs, the optimum The optimization studies for RB-AuNPs and RB-AgNPs were also conducted by using UV-Vis spectrum analysis and visible observations [28].…”

“…The optimization studies for RB-AuNPs and RB-AgNPs were also conducted by using UV-Vis spectrum analysis and visible observations [28]. For RB-AuNPs, the optimum The optimization studies for RB-AuNPs and RB-AgNPs were also conducted by using UV-Vis spectrum analysis and visible observations [28]. For RB-AuNPs, the optimum ratio of SM was 2:8 (Figure 2A), gold salt concentration was 4 mM (Figure 2B), and the reaction took place at room temperature within 5 s. The synthesis was so quick that the rowanberries extract showed complete color change at RT as soon as gold salt was added.…”

Biosynthesis and Cytotoxic Properties of Ag, Au, and Bimetallic Nanoparticles Synthesized Using Lithospermum erythrorhizon Callus Culture Extract

“…Thus, microwave treatment favored the effective biosynthesis of AgNPs using Epicoccum nigrum and Theobroma cacao extracts [84,85]. Microwave irradiation also significantly promoted the synthesis of both AgNPs and AuNPs mediated by eosin-methylene blue agar [86]. Moreover, microwave irradiation was sufficient for the preparation of stable AgNPs without the addition of reducing molecules [87].…”

Gold nanoparticles (GNPs) in biomedical and clinical applications: A review

“…[44] Aside from this traditional synthesis, a few advanced synthesis methods such as ultrasound, ultraviolet (UV) and microwave radiation-induced, reverse micelles, and so on have been reported. [46][47][48][49]…”