One-step warming does not affect the in vitro viability and cryosurvival of cryotop-vitrified donkey embryos

Bottrel, M.; Hidalgo, M.; Mogas, T.; Pereira, B.; Ortiz, Isabel; Díaz-Jiménez, María; Consuegra, C.; Morató, Roser; Dorado, J.

doi:10.1016/j.theriogenology.2020.04.026

Cited by 4 publications

(12 citation statements)

References 51 publications

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“…Although the complete vial of Veterin Chorion contains 3000 IU of hCG, only 1500 IU was used for ovulation induction in this study. This dosage was previously described for jennies by Serres et al [ 68 ], and it was successfully used for inducing ovulation in jennies in later studies [ 61 , 69 , 70 , 71 , 72 ]. However, this is the first study in which this concentration of hCG is compared to 0.75 mg of deslorelin acetate for induction of ovulation in this species.…”

Section: Discussionmentioning

confidence: 99%

Hormonal Management for the Induction of Luteolysis and Ovulation in Andalusian Jennies: Effect on Reproductive Performance, Embryo Quality and Recovery Rate

Bottrel

Ortiz

Hidalgo

et al. 2022

Animals

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Two prostanglandins (luprostiol, LUP, and dinoprost, DIN) and two ovulation-inducing agents (human Chorionic Gonadotropin, hCG, and deslorelin, DES) were evaluated for luteolysis and estrus induction, and for ovulation induction, respectively, in embryo donor jennies. Twenty-six fertile Andalusian jennies were used. In Experiment 1, jennies (n = 112 cycles) were randomly treated with either LUP or DIN after embryo flushing. In Experiment 2, donors (n = 84 cycles) were randomly treated with either hCG or DES to induce ovulation. No differences were found between prostaglandins for all variables studied (prostaglandin–ovulation interval (POI), interovulatory interval (IOI), embryo recovery rate (ERR), positive flushing rate (PFR) and embryo grade (EG)). The ovulation rate was similar for hCG and DES (60.9% vs. 78.7%). However, the interval to ovulation (ITO) was affected (62.61 ± 7.20 vs. 48.79 ± 2.69 h). None of the other variables studied (ERR, PFR and EG) were affected (p > 0.05), except for embryo quality (p = 0.009). In short, both prostaglandins evaluated are adequate to induce luteolysis and estrus. Both ovulation-inducing agents hastened ovulation, but DES seems to be more effective than hCG. Follicular diameter affected the interval from treatment to ovulation, and high uterine edema was related to low embryo quality.

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Section: Discussionmentioning

confidence: 99%

Hormonal Management for the Induction of Luteolysis and Ovulation in Andalusian Jennies: Effect on Reproductive Performance, Embryo Quality and Recovery Rate

Bottrel

Ortiz

Hidalgo

et al. 2022

Animals

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“…Studies in jennies describing a relationship between the size of the embryo and the day of its collection are scarce. Yet, it seems that contrary to mares a high percent of donkey embryos collected during the seventh, or seventh and a half PO days are still < 300 µmØ (Vendramini et al, 1997;Panzani et al, 2012b;Bottrel et al, 2017;Pérez-Marín et al, 2017;Pérez-Marín et al, 2018;Bottrel et al, 2019;Bottrel et al, 2020b;Dorado et al, 2020;Fanelli et al, 2020). When the collection of the donkey embryo is attempted from the eighth PO day or later, its diameter is typically close to or larger than 500 µmØ (Panzani et al, 2012a;Pérez-Marín et al, 2017;Bottrel et al, 2019;Dorado et al, 2020).…”

Section: The Relationship Between Day Of Recovery and Embryo Sizementioning

confidence: 99%

“…The commercial Emcare ® complete flush solution (Eldridge-Panuska et al, 2005) and the Vigro ® flush medium (Hudson et al, 2006) are other alternatives reported as embryo recovery media. In donkeys, the Ringer's lactate solution is the most frequently reported as embryo recovery medium (Panzani et al, 2012a;Peña-Alfaro et al, 2014;Panzani et al, 2016;Pérez-Marín et al, 2018;Bottrel et al, 2019;Bottrel et al, 2020a;Bottrel et al, 2020b). Another reported alternative is the DPBS (Camillo et al, 2010).…”

Section: Intrauterine Infusion Of Liquid Solutions To Collect Equine ...mentioning

confidence: 99%

“…For donkeys, the Emcare Holding Solution ® has been frequently described to wash/manipulate embryos (Camillo et al, 2010;Panzani et al, 2012a;Panzani et al, 2012b;Panzani et al, 2016). The Syngro ® holding medium is employed as an embryo washing solution by several researchers (Bottrel et al, 2017;Pérez-Marín et al, 2017;Bottrel et al, 2019;Bottrel et al, 2020a;Bottrel et al, 2020b). Alternatively, the PBS (Camillo et al, 2010), the Ringer's lactate (Camillo et al, 2010), and the isotonic holding media have been described as embryo washing solutions too (Pérez-Marín et al, 2018).…”

Section: Embryo Washing and Manipulation Mediamentioning

confidence: 99%

“…Information is also scarce when comparing distinct vitrification solutions and the pregnancy rates achieved by employing each of them. Multiple options can be selected as embryo carrier: open pulled straw or OPS (Guignot et al, 2015); fine diameter micropipette (Choi et al, 2011);cryolock (Díaz et al, 2016;Ferris et al, 2016;Wilsher et al, 2019); hemi straw (Sanchez et al, 2017), Fibreplugsolid surface (Pérez-Marín et al, 2018) and cryotop (Bottrel et al, 2019;Bottrel et al, 2020a;Bottrel et al, 2020b). Small equine embryos do not require direct contact with liquid nitrogen to survive the cryopreservation process even if vitrified in large volumes of solution i.e.…”

Section: Introductionmentioning

confidence: 99%

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Colección, vitrificación y transferencia post-calentamiento de embriones equinos producidos in vivo: una revisión de literatura

2022

Abanico Vet

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Small equine embryos, equal or smaller than three hundred micrometers in diameter (≤ 300 µmØ) are collected before the seventh post ovulation (PO) day. Big embryos, larger than three hundred micrometers (>300 µmØ) are recovered during or after the seventh PO day. No experiments test the type of holding and washing solutions and to what extent they influence the embryo post vitrification survival. Further knowledge is needed about the mixture of cryoprotectants; either penetrating or non penetrating and the effect they exert over the embryos post vitrification survival and pregnancy rate (PVSPR). The type of embryo carrier, the size of the embryo and the volume of the vitrification solution vary between small and large embryos. A high temperature transfer index (TTI) optimizes the embryo PVSPR. In equids, the type of holding and transfer solutions used during the post vitrification step is an area scarcely explored. This study endeavors to provide information to help in the adaptation of vitrification protocols depending on the embryo size. An additional objective is to ease the access to data about the solutions employed before and during the vitrification; as well as the solutions used during the embryo warming and transfer steps.

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Vitrification: A Reliable Method for Cryopreservation of Animal Embryos

Cuello,

Gonzalez-Plaza,

Parrilla

et al. 2024

Cryopreservation in Assisted Reproduction

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One-step warming does not affect the in vitro viability and cryosurvival of cryotop-vitrified donkey embryos

Cited by 4 publications

References 51 publications

Hormonal Management for the Induction of Luteolysis and Ovulation in Andalusian Jennies: Effect on Reproductive Performance, Embryo Quality and Recovery Rate

Hormonal Management for the Induction of Luteolysis and Ovulation in Andalusian Jennies: Effect on Reproductive Performance, Embryo Quality and Recovery Rate

Colección, vitrificación y transferencia post-calentamiento de embriones equinos producidos in vivo: una revisión de literatura

Vitrification: A Reliable Method for Cryopreservation of Animal Embryos

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