X-ray-based
analytics are routinely applied in many fields, including
physics, chemistry, materials science, and engineering. The full potential
of such techniques in the life sciences and medicine, however, has
not yet been fully exploited. We highlight current and upcoming advances
in this direction. We describe different X-ray-based methodologies
(including those performed at synchrotron light sources and X‑ray
free-electron lasers) and their potentials for application to investigate
the nano–bio interface. The discussion is predominantly guided
by asking how such methods could better help to understand and to
improve nanoparticle-based drug delivery, though the concepts also
apply to nano–bio interactions in general. We discuss current
limitations and how they might be overcome, particularly for future
use in vivo.