One year daily changes in fecal sexual steroids of two captive female cheetahs ( Acinonyx jubatus ) in Italy

Abstract

Please cite this article as: Vernocchi V, Morselli MG, Faustini M, Gabai G, Da, Dalt L, Luvoni GC, One year daily changes in fecal sexual steroids of two captive female cheetahs (acinonyx jubatus) in italy, Animal Reproduction Science (2010), https://doi.org/10. 1016/j.anireprosci.2018.01.016 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. in the two females, underlying throughout the year a more pronounced rhythmical ovarian estrogenic activity in Geijsha than in Duchessa. However, this statistical approach had a smoothing effect in depicting the hormonal patterns and the number of follicular phases might be lower than that revealed by the iterative method.In this study, RIA on wet feces performed very well to determine sexual steroid concentrations, and an ovarian activity interrupted by anestrous periods along the year in captive cheetahs co-housed in a small group was demonstrated.More information on estrous behavior of captive cheetahs were obtained in this study, but the effects of husbandry and management conditions on natural reproductive physiology of this species remain to elucidate.

Deslorelin subcutaneous implants in Oryx dammah males for reproductive control

“…Before analysis, each fecal sample was thoroughly mixed. To extract steroid hormones from the fecal matrix, 200 mg of the wet samples were double-extracted by boiling in 90% ethanol as described by Vernocchi et al [18]. The ethanolic extracts were dried under nitrogen flow and re-dissolved in 0.25 mL PBS.…”

Reproductive hormones monthly variation in free‐ranging European wildcats: Lack of association with faecal marking

“…The temporal variation in the androgen excretion patterns between species can be explained by the differences in the type of breeding system and the characteristics of the paternal or alloparental care in males (Van Kesteren et al., 2012). Through faecal sampling, sex hormones cycle has been studied in several species of cats, evidencing an ovarian function based on the regular fluctuation of steroids metabolites secretion (Brown et al., 2002; Vernocchi et al., 2018). Through faecal oestrogens metabolites levels, we can distinguish between sexual receptivity (oestrus) and interoestrus periods.…”