One-Year Duration of Immunity Induced by Vaccination with a Canine Lyme Disease Bacterin

LaFleur, Rhonda L.; Callister, Steven M.; Dant, Jennifer C.; Jobe, Dean A.; Lovrich, Steven D.; Warner, Thomas F.; Wasmoen, Terri; Schell, Ronald F.

doi:10.1128/cvi.00524-09

Cited by 26 publications

(27 citation statements)

References 31 publications

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“…As an additional confounding factor, previous vaccination would further complicate diagnosis by an OspCbased antibody test because a widely used commercially available vaccine k prevents B. burgdorferi infection specifically by inducing high levels of anti-OspC antibodies against a widely conserved epitope. 3,4 In contrast, however, the results provided support for the previous observation 17 that dogs with Lyme disease more reliably produced anti-OspF antibodies, and that the response was associated most closely with later stages of the infection. In addition, we demonstrated that IgG antibodies predominated, and that the response was produced most often by dogs harboring spirochetes in the joints.…”

Section: Research-article2015contrasting

confidence: 56%

“…Archived serum samples from 24 healthy laboratory purpose-bred Beagles that had received only placebo prior to challenge with B. burgdorferi-infected ticks in previous studies 3,4 were then evaluated. The dogs were challenged by attaching 10 female and 10 male B. burgdorferi-infected I. scapularis ticks and allowing the female ticks to feed to repletion.…”

Section: Research-article2015mentioning

confidence: 99%

“…In support, we used an OspC that reliably binds anti-OspC antibodies produced by dogs after vaccination with a Lyme disease bacterin 3,4 and those formed during human infection. 18 However, dogs may also be infected with ospC genotypes that differ significantly from those associated commonly with human infection.…”

Section: Research-article2015mentioning

confidence: 99%

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Antibody responses toBorrelia burgdorferiouter surface proteins C and F in experimentally infected Beagle dogs

et al. 2015

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Abstract. Antibody levels to outer surface proteins C and F (OspC and OspF, respectively) in sera collected from laboratory Beagle dogs at 1, 2, and 4 months after challenge with infected black-legged ticks (Ixodes scapularis) were determined. Each dog was confirmed by culture to harbor Borrelia burgdorferi in the skin (n = 10) or the skin and joints (n = 14). Significant levels of immunoglobulin M (Ig)M or IgG anti-OspC antibodies were detected in single serum samples from only 3 (13%) dogs. Similarly, IgM anti-OspF antibodies were detected in only 1 (4%) serum sample collected from a dog with B. burgdorferi in the skin and joints. In contrast, 4 (29%) dogs with skin and joint infections produced IgG anti-OspF antibodies after 2 months, and the response expanded to include 2 (20%) dogs with skin infection and 4 additional dogs with skin and joint infections (overall sensitivity = 62%) after 4 months. The findings failed to support the utility of OspC-based antibody tests for diagnosing canine Lyme disease, but demonstrated that dogs with B. burgdorferi colonizing joint tissue most often produced significant levels of IgG anti-OspF antibodies. Therefore, additional studies to more thoroughly evaluate the clinical utility of OspF-based antibody tests are warranted.

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Section: Research-article2015contrasting

confidence: 56%

Section: Research-article2015mentioning

confidence: 99%

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Antibody responses toBorrelia burgdorferiouter surface proteins C and F in experimentally infected Beagle dogs

et al. 2015

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“…Subsequent studies (17,18) confirmed that the approach provided a high level of protection against canine Lyme disease for at least 1 year. In addition, the investigators demonstrated that the bacterin induced significant amounts of anti-OspC borreliacidal antibodies (17) and postulated that the high level of protection was likely due at least in part to the inclusion of the OspC-producing spirochete.…”

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confidence: 77%

Vaccination with theospA- andospB-Negative Borrelia burgdorferi Strain 50772 Provides Significant Protection against Canine Lyme Disease

LaFleur¹,

Callister

Dant³

et al. 2015

Clin. Vaccine Immunol.

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bBeagles received placebo or ospA-and ospB-negative Borrelia burgdorferi before a tick challenge. A total of 28 (41%) ticks and skin biopsy specimens from each control dog (n ‫؍‬ 10) contained B. burgdorferi. In contrast, 12 (19%) ticks recovered from the vaccine recipients (n ‫؍‬ 10) were infected (P ‫؍‬ 0.0077), and 5 dogs yielded spirochetes from the skin biopsy specimens (P ‫؍‬ 0.0325). In addition, 9 (90%) placebo recipients and 4 (40%) vaccine recipients developed joint abnormalities (P ‫؍‬ 0.0573). Therefore, vaccination with the ospA-and ospB-negative spirochete provided significant protection against Lyme disease. L yme disease, due primarily in the United States to the transmission of Borrelia burgdorferi from infected Ixodes ticks, causes significant morbidity in canines. However, infected dogs rarely develop acute illness (1); instead, the illness manifests as chronic subclinical polyarthritis and/or periarteritis (2, 3) that may progress to frank arthritis (4, 5). In addition, Labrador retrievers, golden retrievers, and Shetland sheepdogs appear more susceptible to kidney nephropathy (6).Canine vaccines that provide protection by inducing antiOspA borreliacidal antibodies to kill B. burgdorferi in the midgut as the infected tick ingests blood (7, 8) have been commercially available for several decades, and the approach has been partially effective (9, 10). However, vaccinated dogs may still become infected, because the expression of OspA is downregulated immediately after the infected tick begins taking a blood meal (11), and the ticks may also transmit ospA-negative Lyme spirochetes (12). In addition, borreliacidal antibodies specific for OspA are genospecies specific (13,14), which is less problematic in the United States, where B. burgdorferi predominates, but significantly impacts efficacy in Europe and Asia, where other genospecies, such as Borrelia garinii, may infect canines (15,16).Researchers therefore sought to overcome the shortcomings of the traditional vaccines by developing a bivalent bacterin comprising a traditional OspA-expressing B. burgdorferi strain and a unique ospA-and ospB-negative B. burgdorferi strain that expressed high levels of OspC. Subsequent studies (17, 18) confirmed that the approach provided a high level of protection against canine Lyme disease for at least 1 year. In addition, the investigators demonstrated that the bacterin induced significant amounts of anti-OspC borreliacidal antibodies (17) and postulated that the high level of protection was likely due at least in part to the inclusion of the OspC-producing spirochete. We therefore examined this possibility more critically by evaluating the protection afforded by vaccination with only the ospA-and ospB-negative B. burgdorferi strain.Vaccination and tick challenge. Two groups (n ϭ 10 in each group) of 8-week-old laboratory-reared beagle puppies were randomized without regard to sex, vaccinated by subcutaneous injection with a 1-ml dose of bacterin or placebo, and boostered after 21 days by subcutaneous injecti...

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“…All the dogs vaccinated with the vaccine containing OspC became positive as previously described with this vaccine. 1,9,10 6,9-11 protection may not be 100%, which emphasizes that vaccines against B. burgdorferi should not be used as the sole prevention strategy; it is imperative to also use acaricides as well. 12 The B. burgdorferi serological responses in the vaccinated dogs that developed infection were similar to those for dogs in experiment 2 that developed infection without being previously vaccinated.…”

Section: Discussionmentioning

confidence: 99%

Multiple antigen target approach using the Accuplex4 BioCD system to detect Borrelia burgdorferi antibodies in experimentally infected and vaccinated dogs

et al. 2015

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Abstract. The primary objective of our study was to optimize detection of serum antibodies to Borrelia burgdorferi using a new commercial automated fluorescence system (Accuplex4 BioCD system, Antech Diagnostics, Lake Success, New York). The system used multiple natural and artificial peptides-outer surface proteins (OspA, OspC, OspF), an outer membrane protein (P39), and a proprietary synthetic peptide (small Lyme peptide [SLP])-and the results were compared with a commercially available enzyme-linked immunosorbent assay that uses a proprietary peptide (C6). Sera from 4 groups were evaluated: dogs vaccinated with 1 of 3 commercially available vaccines (n = 18); dogs infested with adult Ixodes scapularis (black-legged tick; n = 18); dogs previously vaccinated and then infested with I. scapularis (n = 18); and dogs with B. burgdorferi infection that were then vaccinated (n = 14). All of the vaccines evaluated induced OspA responses. However, antibodies against OspF or C6 were not induced in any of the vaccinated dogs. Additionally, the OspF antibodies had 100% sensitivity and specificity when compared to antibodies against C6 peptide. In B. burgdorferi-infected dogs, antibodies against OspC and SLP were detected in serum sooner than antibodies against the other targets. Low levels of antibodies against OspA developed in 6 of 14 B. burgdorferi-infected, unvaccinated dogs and had the shortest duration compared to the other antibodies. Detection of antibody responses to multiple B. burgdorferi targets with this system can be used to help differentiate vaccinated dogs from exposed dogs as well as acute infection from chronic infection.

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One-Year Duration of Immunity Induced by Vaccination with a Canine Lyme Disease Bacterin

Cited by 26 publications

References 31 publications

Antibody responses toBorrelia burgdorferiouter surface proteins C and F in experimentally infected Beagle dogs

Antibody responses toBorrelia burgdorferiouter surface proteins C and F in experimentally infected Beagle dogs

Vaccination with theospA- andospB-Negative Borrelia burgdorferi Strain 50772 Provides Significant Protection against Canine Lyme Disease

Multiple antigen target approach using the Accuplex4 BioCD system to detect Borrelia burgdorferi antibodies in experimentally infected and vaccinated dogs

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