Online high‐pH reversed‐phase liquid chromatography × low‐pH reversed‐phase liquid chromatography tandem electrospray ionization mass spectrometry combined with pulse elution gradient in the first dimension for the analysis of alkaloids in Macleaya cordata (willd.) R. Br

Abstract

K E Y W O R D S alkaloids, comprehensive two-dimensional liquid chromatography, pulsed elution, reversed-phase liquid chromatography × reversed-phase liquid chromatography, traditional Chinese medicine Article Related Abbreviations: 1st D, first dimensional; 2nd D, second dimensional; PE, pulse elution; TS, traditional standard.

The challenge of separating and determining biologically active electrostatically stabilized silanates using the high‐performance liquid chromatography technique

“…This resulted in the shorter retention times and affected the obtained selectivity results for the analyzed ES-silanates, which were significantly better when using the acetonitrile/water mixture (Table 2ac). Satisfactory separation selectivity is often achieved by either changing the composition of the mobile phase or the type of organic eluent, which has a direct impact on the solvation process [41][42][43][44].…”

A new strategy of studying protein–protein interactions: Integrated strong anion exchange/reversed‐phase chromatography/immunoprecipitation coupled with mass spectrometry for large‐scale identification of proteins interact with immunoglobulin G in HeLa cells

“…RPLC has many advantages such as high capacity, online desalting, and well compatibility with MS detection; therefore, it is usually employed as the second‐dimension separation system [18]. In this study, C4 analytical column and 70 min nonlinear gradient were adopted.…”

Gain deeper insights into traditional Chinese medicines using multidimensional chromatography combined with chemometric approaches

“…var. spinosa (Bunge) Hu ex H. F. Chou (Suanzaoren): seeds PAC (2.1 mm × 150 mm, 5 μm) BEH Shield C 18 (3 mm × 50 mm, 1.7 μm） Q-TOF-MS Zhao et al, 2023b Dendrobium species SB C 18 (2.1 mm × 100 mm, 1.8 μm) Poroshell 120 Bonus (3.0 mm × 50 mm, 1.8 μm) Q-TOF-MS Dong et al, 2020 Alkaloids in M. cordata : roots Symmetry Shield C 18 (150 mm × 4.6 mm, 5 μm) Ultimate XB C 18 (100 mm × 4.6 mm, 5 μm) MS Hu et al, 2020 Niuhuang Shangqing Pill (NSP) ACQUITY UPLC HSS CYANO (100 mm × 2.1 mm, 1.8 μm) Kinetex XB-C 18 (50 mm × 3 mm, 2.6 μm) UV Wu, Liang, Liang, & Xiong, 2022 Xiaoer-Feire-Kechuan oral liquid CSH C 18 (2.1 mm × 100 mm, 1.7 μm) Phenyl-Hexyl (3.0 mm × 50 mm, 2.7 μm) MS Shang et al, 2021 …”