Online naphazoline quality control by micellar‐enhanced spectrofluorimetry

Peralta, Cecilia; Silva, Raúl Alejandro; Fernández, Liliana Patricia; Masi, A.

doi:10.1002/bio.1297

Cited by 6 publications

(6 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increase in fluorescence in micellar media may be attributed to stabilization/protection of the excited singlet state, which hinders decay by quenching, and other nonradiative deactivation processes (17,18). This general observation has been used to improve the performance of spectrofluorimetric methods of various analytes (19,20). The fluorescence properties of SG in various micellar media were studied and SDS was selected because it gave the largest enhancement of the fluorescence intensity (~200% enhancement) compared with aqueous solution.…”

Section: Resultsmentioning

confidence: 99%

Micelle‐enhanced spectrofluorimetric method for determination of sitagliptin and identification of potential alkaline degradation products using LC‐MS

et al. 2013

View full text Add to dashboard Cite

A novel, quick, simple and highly sensitive spectrofluorimetric method was developed and validated for the determination of sitagliptin (SG) in its pharmaceutical formulations. The proposed method is based on investigation of the fluorescence spectral behavior of sitagliptin in an SDS micellar system. In an aqueous solution of phosphate buffer pH 4.0, the fluorescence intensity of SG in the presence of SDS was greatly enhanced, by 200%, i.e. twofold enhancement. The fluorescence intensity of SG was measured at 300 nm after excitation at 270 nm. The method showed good linearity in the range 0.03-10.0 µg/mL with a good correlation coefficient (r = 0.9998). The limits of detection and quantitation values were 5.31 and 16.1 ng/mL, respectively. The proposed method was successfully applied to the analysis of SG in its single and co-formulated commercial tablets; the results were in good agreement with those obtained using a reference method. Application of the proposed method was extended to stability studies of SG after exposure to different forced degradation conditions according to the ICH guidelines, such as acidic, alkaline, thermal, photo- and oxidative stress. The chemical structure of certain potential degradation products (DPs) were investigated using LC-MS.

show abstract

Section: Resultsmentioning

confidence: 99%

Micelle‐enhanced spectrofluorimetric method for determination of sitagliptin and identification of potential alkaline degradation products using LC‐MS

et al. 2013

View full text Add to dashboard Cite

show abstract

“…[4][5][6] The chemical structures for both drugs are depicted in Fig.1. Until now, NFZ was only analyzed in combination with other drugs than ANZ in ophthalmic formulations using capillary electrophoresis, 7 gas chromatography, 8 spectrofluorimetery, 9 phosphometric, 10 electrochemical 11 detectors coupled to HPLC. 12,13 To the best of our knowledge, only one first-derivative spectrometric assay for the simultaneous determination of ANZ and NFZ in ophthalmic formulations was published in the literature.…”

Section: Introductionmentioning

confidence: 99%

Stability Indicating UHPLC-PDA Assay for Simultaneous Determination of Antazoline Hydrochloride and Naphazoline Hydrochloride in Ophthalmic Formulations

Ahmed¹,

Ali²,

Farooq³

et al. 2017

ACSi

View full text Add to dashboard Cite

In the present study, a newly developed method based on ultrahigh performance liquid chromatography (UHPLC) was optimized for the simultaneous determination of antazoline hydrochloride (ANZ) and naphazoline hydrochloride (NFZ) in ophthalmic formulations. Isocratic separation of ANZ and NFZ was performed at 40 °C with an ACE Excel 2 C18-PFP column (2 μm, 2.1 × 100 mm) at a flow rate of 0.6 mL min -1 whereas the mobile phase consisted of acetonitrile/phosphate buffer (60:40, v/v, pH 3.0) containing 0.5% triethylamine. Both analytes were detected at a wavelength of 285 nm and the injection volume was 1.0 μL. The overall run time per sample was 4.5 min with retention time of 0.92 and 1.86 min for NFZ and ANZ, respectively. The calibration curve was linear from 0.500-100 μg mL -1 for ANZ and NFZ with a correlation coefficient ≥ 0.9981 while repeatability and reproducibility (expressed as relative standard deviation) were lower than 1.28 and 2.14%, respectively. In comparison with high-performance liquid chromatography (HPLC), the developed UHPLC method had remarkable advantages over HPLC as the run time was significantly reduced by 3.4-fold with a 5-fold decreased solvent consumption. Forced degradation studies indicated a complete separation of the analytes in the presence of their degradation products providing high degree of method specificity. The proposed UHPLC method was demonstrated to be simple and rapid for the determination of ANZ and NFZ in commercially available ophthalmic formulations providing recoveries between 99.6 and 100.4%.

show abstract

“…Various analytical methods have been proposed for determination of NH, namely spectrofluorimetry , phosphorimetry , spectrophotometry , electrochemistry , liquid chromatography , capillary electrophoresis (CE) , and CL . To the best of our knowledge, up to now only two CL methods have been reported for determination of NH which may be considered as preliminary attempts to approach the subject.…”

Section: Introductionmentioning

confidence: 99%

Determination of naphazoline hydrochloride in biological and pharmaceutical samples by a quantum dot‐assisted chemiluminescence system using response‐surface methodology

Imani-Nabiyyi

Sorouraddin

2014

Luminescence

View full text Add to dashboard Cite

A simple and highly sensitive chemiluminescence (CL) method is reported for the determination of naphazoline hydrochloride (NH). It was found that the weak CL from the reaction of luminol and KIO4 in an alkaline medium could be highly amplified by cysteine-capped cadmium telluride quantum dots (QDs) and the enhanced CL was effectively quenched by NH and this finding was utilized as a basis for the determination of NH. The QDs were synthesized in aqueous medium and characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), and UV-vis and photoluminescence spectroscopy. A possible mechanism was proposed for the CL system based on radical identification experiments, along with CL spectrum of the system. The experimental parameters were optimized by the reliable response surface methodology (RSM). Under the optimized experimental conditions, the proposed method allowed the determination of NH over the range of 5.0 × 10(-10) -2.0 × 10(-7) mol/L (r(2) = 0.9993, n = 10). The precision (RSD%) of the method, obtained from five replicate determinations of 2.0 and 150 nmol/L NH, was found to be 1.0% and 1.3%, respectively. The method was successfully applied to the determination of NH in pharmaceutical formulations and human urine and serum samples with results corroborated with the aid of those obtained from a standard method.

show abstract

Online naphazoline quality control by micellar‐enhanced spectrofluorimetry

Cited by 6 publications

References 17 publications

Micelle‐enhanced spectrofluorimetric method for determination of sitagliptin and identification of potential alkaline degradation products using LC‐MS

Micelle‐enhanced spectrofluorimetric method for determination of sitagliptin and identification of potential alkaline degradation products using LC‐MS

Stability Indicating UHPLC-PDA Assay for Simultaneous Determination of Antazoline Hydrochloride and Naphazoline Hydrochloride in Ophthalmic Formulations

Determination of naphazoline hydrochloride in biological and pharmaceutical samples by a quantum dot‐assisted chemiluminescence system using response‐surface methodology

Contact Info

Product

Resources

About