Online solid-phase extraction liquid chromatography–electrospray-tandem mass spectrometry analysis of buprenorphine and three metabolites in human urine

Liu, An-Chan; Lin, Tzuen-Yeuan; Su, Lien-Wen; Fuh, Ming‐Ren

doi:10.1016/j.talanta.2007.10.050

Cited by 11 publications

(22 citation statements)

References 15 publications

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“…Among these, only four manuscripts describe a method for the simultaneous determination of BUP, NBUP and their glucuronides, one in plasma [36], two in urine [28, 30], and one in both matrices [24]. …”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of buprenorphine, norbuprenorphine, buprenorphine-glucuronide and norbuprenorphine-glucuronide in human umbilical cord by liquid chromatography tandem mass spectrometry

Concheiro

Shakleya

Huestis

2009

Forensic Science International

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A LCMS method was developed and validated for the simultaneous determination of buprenorphine (BUP), norbuprenorphine (NBUP), buprenorphine glucuronide (BUP-Gluc) and norbuprenorphine glucuronide (NBUP-Gluc) in human umbilical cord. Quantification was achieved by selected ion monitoring of precursor ions m/z 468.4 for BUP; 414.3 for NBUP; 644.4 for BUP-Gluc and 590 for NBUP-Gluc. BUP and NBUP were identified by MS 2 , with m/z 396, 414 and 426 for BUP, and m/ z 340, 364 and 382 for NBUP. Glucuronide conjugates were identified by MS 3 with m/z 396 and 414 for BUP-Gluc and m/z 340 and 382 for NBUP-Gluc. The assay was linear 1-50 ng/g. Intra, interday and total assay imprecision (%RSD) were <14.5%, and analytical recovery ranged from 94.1% to 112.3% for all analytes. Extraction efficiencies were >66.3%, and process efficiency >73.4%. Matrix effect ranged, in absolute value, from 3.7% to 27.4% (CV<21.8%, n=8). The method was selective with no endogenous or exogenous interferences from 41 compounds evaluated. Sensitivity was high with limits of detection of 0.8 ng/g. In order to prove method applicability, an authentic umbilical cord obtained from an opioid-dependent pregnant woman receiving BUP pharmacotherapy was analyzed. Interestingly, BUP was not detected but concentrations of the other metabolites were NBUP-Gluc 13.4 ng/g, BUP-Gluc 3.5 ng/g and NBUP 1.2 ng/g.

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Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of buprenorphine, norbuprenorphine, buprenorphine-glucuronide and norbuprenorphine-glucuronide in human umbilical cord by liquid chromatography tandem mass spectrometry

Concheiro

Shakleya

Huestis

2009

Forensic Science International

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“…The LODs obtained for buprenorphine and its metabolites have been reported to be below 0.8 ng/g in the placenta and below 0.5 ng/ml in plasma and urine. A recently reported online SPE-LC-ESI/MS/MS method for urine was fast and reliable, with RSDs of 0 -10 % [26]. Only minimal ion suppression in ESI, a less than 7 % decline in the response compared with that of a pure water sample, was detected.…”

Section: Quantitative Lc/ms Analysismentioning

confidence: 95%

“…Both ESI-and APCI-based methods are utilized, thus showing the freedom of selection of suitable ionization techniques for opiates. Quantitative LC-ESI/MS/MS methods have been developed for the simultaneous determination of buprenorphine, norbuprenorphine, buprenorphine-3-glucuronide (BUP-G), and norbuprenorphine-3-glucuronide (NBUP-G) in plasma [159][160], urine [16,26], and placenta [161]. The LODs obtained for buprenorphine and its metabolites have been reported to be below 0.8 ng/g in the placenta and below 0.5 ng/ml in plasma and urine.…”

Section: Quantitative Lc/ms Analysismentioning

confidence: 99%

“…SPE can be easily automated and mixed-mode extraction was used in an online 96-well SPE method for the extraction of morphine and its glucuronides from human plasma [25]. Another online SPE method was utilized for the extraction of buprenorphine and its metabolites from human urine prior to LC-ESI-MS/MS analysis [26]. The online SPE allowed considerable shortening of the overall analysis time and reduction of sample volume, while still providing the same sensitivity levels as offline SPE.…”

mentioning

confidence: 99%

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Direct Analysis of Glucuronides with Liquid Chromatography-Mass Spectrometric Techniques and Methods

Ketola¹,

Hakala

2010

CDM

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Glucuronidation is one of the main phase II metabolic reactions in humans and animals. A variety of analytical techniques and methods have been used for the detection and quantification of glucuronides of both endogenous and xenobiotic compounds from different biological samples of humans and animals. Drug metabolism has been extensively studied with both in vitro and in vivo experiments under various conditions. The purpose of this review is to explore in detail the benefits and drawbacks of different liquid chromatography-mass spectrometric (LC/MS) methods and techniques in detection and identification of all forms of glucuronide conjugates from in vitro, biological, and environmental samples. The entire analytical procedure is covered, from sample treatment, separation, and ionization to qualitative and quantitative analyses. The aim of this review is not to cover every published paper where glucuronides are identified and/or quantified, but rather to focus on special cases where a new analytical approach or technical development has led to a better, more specific, or more comprehensive detection, identification, or quantitation of glucuronide conjugates.

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“…Various methods of sample preparation have also been described, including protein precipitation [40], liquid/liquid extraction [39], and solid-phase extraction [37,41,42]. The most sensitive of the above assays generally have a lower limit of quantification of approximately 0.1 ng/mL for buprenorphine and 0.25 ng/mL for norbuprenorphine, and the glucuronide metabolites are often not analyzed.…”

Section: Introductionmentioning

confidence: 99%

High-sensitivity analysis of buprenorphine, norbuprenorphine, buprenorphine glucuronide, and norbuprenorphine glucuronide in plasma and urine by liquid chromatography–mass spectrometry

Regina

Kharasch

2013

Journal of Chromatography B

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A new method using ultra-fast liquid chromatography and tandem mass spectrometry (UFLC–MS/MS) was developed for the simultaneous determination of buprenorphine and the metabolites norbuprenorphine, buprenorphine-3β-glucuronide, and norbuprenorphine-3β-glucuronide in plasma and urine. Sample handling, sample preparation and solid-phase extraction procedures were optimized for maximum analyte recovery. All four analytes of interest were quantified by positive ion electrospray ionization tandem mass spectrometry after solid-phase microextraction. The lower limits of quantification in plasma were 1 pg/mL for buprenorphine and buprenorphine glucuronide, and 10 pg/mL for norbuprenorphine and norbuprenorphine glucuronide. The lower limits of quantitation in urine were 10 pg/mL for buprenorphine, norbuprenorphine and their glucuronides. Overall extraction recoveries ranged from 68–100% in both matrices. Interassay precision and accuracy was within 10% for all four analytes in plasma and within 15% in urine. The method was applicable to pharmacokinetic studies of low-dose buprenorphine.

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Online solid-phase extraction liquid chromatography–electrospray-tandem mass spectrometry analysis of buprenorphine and three metabolites in human urine

Cited by 11 publications

References 15 publications

Simultaneous quantification of buprenorphine, norbuprenorphine, buprenorphine-glucuronide and norbuprenorphine-glucuronide in human umbilical cord by liquid chromatography tandem mass spectrometry

Simultaneous quantification of buprenorphine, norbuprenorphine, buprenorphine-glucuronide and norbuprenorphine-glucuronide in human umbilical cord by liquid chromatography tandem mass spectrometry

Direct Analysis of Glucuronides with Liquid Chromatography-Mass Spectrometric Techniques and Methods

High-sensitivity analysis of buprenorphine, norbuprenorphine, buprenorphine glucuronide, and norbuprenorphine glucuronide in plasma and urine by liquid chromatography–mass spectrometry

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