Ontogenèse gonadique et différenciation sexuelle du Loup Dicentrarchus labrax, en conditions d'élevage

Roblin, C.; Brusle, J.

doi:10.1051/rnd:19830110

Cited by 57 publications

(48 citation statements)

References 7 publications

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“…Elles ne se manifestent qu'au 8 e jour chez Brachydanio (Takahashi, 1977) et Gasterosteus (Shimizu et Takahashi, 1980) entre le 20 e et le 40 e jour chez Cyprinus (Ryazantseva et Sakun, 1980). Chez Oryzias (Hamaguchi, 1979) Ohno, 1966 ;Zamboni et al, 1979 ;Byskov, 1978) que chez les Poissons (Harrington, 1974(Harrington, et 1975 Lepori (1980) (Roblin et Bruslé, 1983). Dans l'ovaire présomptif de la Truite, à l'échelle ultrastructurale, Upadhyay (1977) (Cala, 1970 ; Nakamura et Lepori, 1980), cette crête génitale est constituée par un cordon continu de cellules somatiques (mésothéliales, fibroblastiques...) dans lequel des CGP sont distribuées de façon discontinue (Johnston, 1951 ;Swarup, 1958 ;Belsare, 1974 ;Roblin et Bruslé, 1983).…”

unclassified

“…Chez Oryzias (Hamaguchi, 1979) Ohno, 1966 ;Zamboni et al, 1979 ;Byskov, 1978) que chez les Poissons (Harrington, 1974(Harrington, et 1975 Lepori (1980) (Roblin et Bruslé, 1983). Dans l'ovaire présomptif de la Truite, à l'échelle ultrastructurale, Upadhyay (1977) (Cala, 1970 ; Nakamura et Lepori, 1980), cette crête génitale est constituée par un cordon continu de cellules somatiques (mésothéliales, fibroblastiques...) dans lequel des CGP sont distribuées de façon discontinue (Johnston, 1951 ;Swarup, 1958 ;Belsare, 1974 ;Roblin et Bruslé, 1983). Elles peuvent être plus concentrées en direction crâniale (Lissia-Frau, 1968, chez Perca ;Davies et Ryazantseva et Sakun, 1980, chez (Hogan, 1973 ; Egami, 1973 ;Bruslé et Bruslé, 1978b ;Bruslé, 1980Bruslé, , 1982 sont toujours entourées par des cellules somatiques qui leur sont annexées (Kanobdee, 1975 ;Upadhyay, 1977 ;Bruslé, 1982 (Bruslé, 1982 (Harrington, 1975), Platycephalidés (Fujii, 1971) et surtout Serranidés (Bruslé, 1982) et Sparidés (D'Ancona, 1956 ;Lissia-Frau et Casu, 1968, MaloMichele, 1977Lepori, 1980 Casu, 1968 ;Lepori, 1980) (Harrington, 1975) et chez Serranus (Bruslé, 1982…”

unclassified

“…C'est pourquoi, le critère de différenciation sexuelle précoce généralement adopté, à l'échelle de la microscopie photonique, est celui de l'entrée en prophase méiotique qui caractérise les ovocytes par opposition à une méiose spermatocytaire beaucoup plus tardive. La reconnaissance d'ovocytes préméiotiques et méiotiques constituerait alors la preuve d'une différenciation en direction ovarienne chez la Lamproie (Hardisty, 1965), la Truite (Vlad, 1976 ;Takashima et al, 1980), le Saumon (Nakamura et al, 1974 ;Magomedov et al, 1979), Oryzias (Onitake, 1972 ;Satoh, 1974 ; Egami, 1972Egami, et 1973Yoshikawa et Oguri, 1981), Hemihaplochromis (Muller, 19691, Poecilia (Takahashi, 1975 a, b), Tilapia (Nakamura et Yoshikawa et Oguri, 1978a, b) et Brachydanio (Takahashi, 1977 Mires, 1976 ; , Cyprinus (Davies et Takashima, 1980), Dicentrarchus (Roblin et Bruslé, 1983). De tels ovocytes (testisova, oocyte-like-cells) parfaitement normaux à l'échelle ultrastructurale (Bruslé, 1982) (Lebrun, 1977), l'augmentation du nombre des cellules sexuelles est fonction du sexe présumé (1 500 chez les femelles présomptives et moins de 1 000 chez les mâles présomp-tifs).…”

unclassified

“…De plus, cette atrésie est tardive dans la forme « landlocked » de la Lamproie Petromyzon marinus (Hardisty, 1965(Hardisty, et 1969Hardisty et Cosh, 1966) Takahashi, 1977 ; Anguilla : Kuhlman, 1975 ;Lampetra : Busson-Mabillot, 1965 ;Hardisty, 1965Hardisty, , 1969Hardisty, et 1970. La (Gamo, 1961), Salmo (Lebrun, 1977), Tilapia (Jensen et , Cyprinus (Davies et Takashima, 1980) et Dicentrarchus (Roblin et Bruslé, 1983 (Ashby, 1957 ;Magomedov et al, 1979 ;Takashima et al, 1980), Tilapia (Nakamura et , Anguilles (Bryliriska et al, 1978), Gasterosteus (Shimizu et Takahashi, 1980), Cyprinus (Davies et , Muges (Bruslé et Bruslé, 1978a (Kuhlman, 1975 ;Bieniarz et al, 1981 ;Dutta, 1979, cité par Shelton et al, 1981Lebrun, 1977 (Persov, 1972 (Hardisty, 1970 (Johnston, 1951), Carassius (Takahashi et Takano, 1971), Poecilia (Takahashi, 1974), ldus (Cala, 1976), Tilapia (Nakamura et Yoshikawa et Oguri, 1978a, b) et Oryzias (Yamamoto, 1953(Yamamoto, et 1959Yamamoto et Matsuda, 1963). Chez cette dernière espèce, le gradient n'a pas été confirmé par Yoshikawa et Oguri (1979,19811 …”

unclassified

“…Chez cette dernière espèce, le gradient n'a pas été confirmé par Yoshikawa et Oguri (1979,19811 (Mezhnin, 1978). Un net gradient caudocranien, tant de colonisation des crêtes génitales par les CGP que de différen-ciation ovarienne ou testiculaire, est évident chez le Loup Dicentrarchus (Roblin et Bruslé, 1983 (Roblin, 1980 (Robertson, 1953 ;Ashby, 1957) Sakun, 1980). Au contraire, chez Micropterus, la partie la plus développée de la gonade se situe dans la zone postérieure (Johnston, 1951 Harrington, 1975 ;Serranus : Bruslé, 1982, 1983 que chez les hermaphrodites successifs (Sparidés tels que la Daurade, Labridés tels que la girelle : Reinboth, 1962Reinboth, , 1970 (Atz, 1964) mais aussi chez les hermaphrodites synchrones (Harrington, 1975 ;Bruslé, 1983 (De Smet, 1975 ;Lebrun, 1977 ;Yoshikawa et Oguri, 1978a, b).…”

unclassified

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La gonadogenèse des Poissons

Brusle¹,

Bruslé²

1983

Reprod. Nutr. Dévelop.

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unclassified

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La gonadogenèse des Poissons

Brusle¹,

Bruslé²

1983

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Effects of rearing temperature on sex differentiation in the European sea bass (Dicentrarchus labrax L.)

et al. 1998

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Under culture conditions, sex ratios in the European sea bass (Dicentrarchus labrax L.) are consistently skewed in favor of males. Moreover, no heteromorphic sex chromosomes have been shown for this species. This bias towards males could be due to a possible effect of environmental factors on sex differentiation, as has already been described in other fish species. To test this hypothesis, sexually undifferentiated sea bass were reared under two different thermal regimes or two different photoperiodic regimes, resulting in four groups: Group SP = short photoperiod (9 hours light [L]:15 hours dark [D]), Group LP = long photoperiod (15 L:9 D), both reared under natural temperature (24°C, average); Group LT = low temperature (15°C), Group HT = high temperature (25°C), both reared under natural photoperiod (13.5 L:10.75 D, average). Treatments lasted 80 days, from 57 to 137 days post fertilization, coinciding with the time when gonads are more sensitive to the action of exogenous sex steroids. The resulting sex proportions were determined by periodic histological examinations of the gonads during the first 2 years of age. No females were found in group LT at any sampling, whereas in the other groups, female proportions never reached values higher than 27%. Moreover, sex differentiation in group LT was delayed with respect to the other groups, as no fish completed sex differentiation until 16.4 months of age, compared to the other groups in which some individuals completed sex differentiation at 9 months of age. In addition, growth was significantly (P > 0.05) delayed in group LT with respect to the other three experimental groups and especially marked during the first year of life. Furthermore, the low values for the gonadosomatic index (GSI), together with the decrease of the number of spermatogenic males, denote a delay in gonadal maturation with respect to the rest of the experimental groups. The results of this study suggest that temperature can play an important role in the process of sex differentiation and growth of the sea bass. However, the mechanism(s) underlying this sexual lability remain unknown. J. Exp. Zool. 281:207–216, 1998. © 1998 Wiley‐Liss, Inc.

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Spermatogonia of rainbow trout: I. morphological characterization, mitotic activity, and survival in primary cultures of testicular cells

Loir

1999

Mol. Reprod. Dev.

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Prerequisites of developing in vitro studies for a better understanding of the control mechanisms underlying the proliferation and differentiation of spermatogonia (Go) in the teleost testis are: (1) to be able to identify the different types of Go; (2) to maintain in culture the structural relationships occurring in situ between the various testicular cell types, as intact as possible; and (3) to know how the Go survive and proliferate in culture for several days. After very gentle homogenization of trout testes treated with collagenase, a cell suspension containing mainly spermatocysts (one or several Sertoli cells enclosing one Go or a clone of germ cells) and clusters of myoid cells and Leydig cells was seeded in culture onto a laminin plus fibronectin coating. After 4.5–6 days in culture, then staining with May‐Grünwald and Giemsa reagents, the determination of the nuclear and cellular size of the various Go and of the number of Go present in clones has enabled the identification of two types of large Go, in pairs or alone (Go A) and six successive types of smaller Go (Go B). Cell viability determination by staining with Rhodamine 123/propidium iodide (PI)/Hoechst 33342 and with FITC‐Annexin V/PI indicated that after 5–7 days in culture, all the somatic cells and most of the Go were viable. Only some of the Go, mainly among the most differentiated ones, underwent apoptosis, as it was the case for a number of spermatocytes and spermatids increasing with the time in culture. Brdu labelling and 3H‐Thymidine (3H‐Tdr) incorporation indicated that the proliferative activity of Go was at a maximum after 4.5 days in culture and that the response to at least two molecules (QAYL‐IGF‐I and GTH‐I) remained unchanged between 3 and 6 days. As only very scarce somatic cells from immature/spermatogenetic testes synthesized DNA up to 6 days in culture, the measurement of 3H‐Tdr incorporation by cells from such testes reliably reflected synthesis of DNA by only the Go (and eventually also by primary spermatocytes when they are present). In conclusion, this study provides information allowing a detailed analysis of the events related with the mitotic phase of spermatogenesis in the trout and it establishes that primary cultures of testicular cells carried out in the reported conditions represent a useful tool to develop an analysis of the mechanisms participating in the control of this phase. Mol. Reprod. Dev. 53:422–433, 1999. © 1999 Wiley‐Liss, Inc.

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Ontogenèse gonadique et différenciation sexuelle du Loup Dicentrarchus labrax, en conditions d'élevage

Abstract: Summary. Gonadal

Cited by 57 publications

References 7 publications

La gonadogenèse des Poissons

La gonadogenèse des Poissons

Effects of rearing temperature on sex differentiation in the European sea bass (Dicentrarchus labrax L.)

Spermatogonia of rainbow trout: I. morphological characterization, mitotic activity, and survival in primary cultures of testicular cells

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