Ontogenetic and temperature-dependent changes in tolerance to hypoxia and hydrogen sulfide during the early life stages of the Manila clam Ruditapes philippinarum

Kodama, Keita; Waku, Mitsuyasu; Sone, Ryota; Miyawaki, Dai; Ishida, Toshiro; Akatsuka, Tetsuji; Horiguchi, T.

doi:10.1016/j.marenvres.2017.12.019

Cited by 19 publications

(14 citation statements)

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“…Moreover, ocean acidification, increased water temperatures, and hypoxic events are increasing worldwide problems (Steckbauer et al., 2015). The molecular mechanisms underlying the tolerance of benthic organisms for diverse biotic and abiotic factors, such as parasites, ocean acidification, and hypoxia, have received much attention in recent years (Lim et al., 2006, Shimokawa et al., 2010, Zhao et al., 2018, Kodama et al., 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Bivalve mollusks have a biphasic life cycle, in which planktonic larvae (trochophores and veligers) settle (as pediveliger larvae) and undergo metamorphosis (to the juvenile stage). The transition of benthic bivalves from a pelagic to a benthic lifestyle during their early development, their burrowing to avoid predation, and their tolerance of hypoxic environments have been attractive characteristics for research (Tamai, 1993, Kodama et al., 2018). However, knowledge of the underlying molecular mechanisms that regulate these processes is still very limited (Findlay and Battin, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Clam Genome Sequence Clarifies the Molecular Basis of Its Benthic Adaptation and Extraordinary Shell Color Diversity

et al. 2019

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SummaryRuditapes philippinarum is an economically important bivalve with remarkable diversity in its shell coloration patterns. In this study, we sequenced the whole genome of the Manila clam and investigated the molecular basis of its adaptation to hypoxia, acidification, and parasite stress with transcriptome sequencing and an RNA sequence analysis of different tissues and developmental stages to clarify these major issues. A number of immune-related gene families are expanded in the R. philippinarum genome, such as TEP, C3, C1qDC, Hsp70, SABL, and lysozyme, which are potentially important for its stress resistance and adaptation to a coastal benthic life. The transcriptome analyses demonstrated the dynamic and orchestrated specific expression of numerous innate immune-related genes in response to experimental challenge with pathogens. These findings suggest that the expansion of immune- and stress-related genes may play vital roles in resistance to adverse environments and has a profound effect on the clam's adaptation to benthic life.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Clam Genome Sequence Clarifies the Molecular Basis of Its Benthic Adaptation and Extraordinary Shell Color Diversity

et al. 2019

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“…The environmental stressors such as microplastic, heavy metal, sulphide, ammonia and dissolved oxygen are common aspects of aquaculture (Kodama et al, 2018; Li et al, 2017; Sui et al, 2016; Wang et al, 2009; Zhang et al, 2021). It is able to stimulate the aquatic organisms to produce intracellular reactive oxygen species (ROS) (Sevcikova et al, 2011), when the concentration of ROS overwhelms the preventive capacity of antioxidant defence systems, resulting in oxidative stress and even highly injury further to organisms such as protein oxidation, lipid peroxidation (e.g.…”

Section: Discussionmentioning

confidence: 99%

Effects of adding EM bacteria and mechanical aeration on water quality, growth and antioxidant status of Meretrix meretrix and Exopalaemon carinicauda farmed in the clam–shrimp polyculture system

Zhang¹,

Wu²,

Yu³

et al. 2021

Aquaculture Research

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In coastal China, the clam–shrimp polyculture system is a new model of seawater aquaculture. A 60‐day rearing experiment was performed to evaluate the effects of commercial effective microorganisms (EM) bacteria liquid, containing bacillus, photosynthetic bacteria and lactic acid bacteria and mechanical aeration on water quality and their growth performance and antioxidant status. A 2 × 2 layout was designed, including four treatment groups were abbreviated as O (non‐supplementation of EM bacteria liquid and no mechanical aeration), A (supplementation of EM bacteria liquid), B (mechanical aeration) and AB (supplementation of EM bacteria liquid and mechanical aeration). AB group had significantly lower concentration of ammonia, nitrite, nitrate, total inorganic nitrogen (TIN), total inorganic phosphorus (TIP) and chemical oxygen demand (CODMn) than O group (p < 0.05) on the 60th day. Clams and shrimps farmed in the interaction group (AB group) had significantly higher body weight than O group on the 60th day (p < 0.05). Furthermore, body weight of clams had no significant differences between single factor groups (A and B groups) and O group (p > 0.05), while shrimps had significantly higher body weight in single factor groups (A and B groups) than O group on the 60th day (p < 0.05). Weight gain and specific growth rate of clams had no significant differences among different groups at the same time (p > 0.05). Shrimps reared in B and AB groups showed significantly higher T‐SOD activities, T‐AOC and CAT activities than those reared in O and A groups (p < 0.05). There were significantly lower MDA activities in B and AB groups than O group (p < 0.05), while no significant difference was observed in hepatic MDA activities in A group than other groups (p > 0.05). There were no significant differences in T‐SOD activities of clams between the four groups (p > 0.05). Clams farmed in AB group had lower MDA activities than other groups (p < 0.05). Clams farmed in AB group had significantly higher T‐AOC activities than O and B groups (p < 0.05). Although B group in T‐AOC activities was significantly lower than A group (p < 0.05), it had no significant difference than O group (p > 0.05). Finally, clams farmed in A and AB groups had significantly higher CAT activities than O group (p < 0.05), while they had no significant difference than B group (p > 0.05). In conclusion, single EM bacteria addition or mechanical aeration had limited function in improving water quality, growth performance and antioxidant capacity of clams and shrimps. In contrast, the interactive impact of adding EM bacteria and mechanical aeration could be competent for this task. Therefore, combined adding EM bacteria and mechanical aeration should be an effective way to improve water quality and their growth performance and immune function in the clam–shrimp polyculture system.

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“…Although the adaptive capacity to low dissolved oxygen is variable among aquatic animals, hypoxia may have negative or detrimental effects on the development, growth, reproduction and survival of the aquatic organisms (Camacho‐Jiménez et al, 2018; Vaquer‐Sunyer & Duarte, 2008). As marine benthic organisms, clams may face changes in oxygen level in their natural environment, which can lead to severe hypoxic conditions (Kodama et al, 2018). Some clams can cope with hypoxia stress by adjusting physiological, biochemical and behavioural alteration (Kozuki et al, 2013; Nie et al, 2018; Sun et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Unravelling the characterization of hypoxia‐inducible factor‐1α ( HIF‐1α ) and antioxidant enzymes in clam Cyclina sinensis in response to hypoxia

Ni¹,

Wang²,

Xie³

et al. 2022

Aquaculture Research

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Aquatic hypoxia has negative or detrimental effects on clams. To cope with hypoxia stress, a complex series of physiological and biochemical strategies can be employed in clams. Hypoxia‐inducible factor‐1α (HIF‐1α) and antioxidant enzymes are commonly found in aquatic animals as an adaptation against hypoxia. In the present study, we cloned the HIF‐1α sequence in clam Cyclina sinensis, and it consists of 2654 base pairs (bp) carrying a single open‐reading frame that encompasses 2235 bp of the coding region. The predicted C. sinensis HIF‐1α protein (744 amino acids) has the conserved HLH‐PAS domain, which contained one N‐terminal basic helix–loop–helix (bHLH) domain and two Per‐ARNT‐Sim A/B (PAS) domains, followed by PAS‐associated C‐terminal (PAC) domain. HIF‐1α is expressed predominantly in gills. Exposed to hypoxia, the transcript level of HIF‐1α was significantly up‐regulated in response to hypoxia, and then, the transcript level declined continuously with the time elapsed. The antioxidant enzymes (SOD, CAT and GSH‐PX) in gills were increased initially and then decreased after acute hypoxia exposure. Our study indicates that the HIF‐1α gene and antioxidant enzymes may play an important role in cope with acute hypoxia exposure.

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Ontogenetic and temperature-dependent changes in tolerance to hypoxia and hydrogen sulfide during the early life stages of the Manila clam Ruditapes philippinarum

Cited by 19 publications

References 13 publications

Clam Genome Sequence Clarifies the Molecular Basis of Its Benthic Adaptation and Extraordinary Shell Color Diversity

Clam Genome Sequence Clarifies the Molecular Basis of Its Benthic Adaptation and Extraordinary Shell Color Diversity

Effects of adding EM bacteria and mechanical aeration on water quality, growth and antioxidant status of Meretrix meretrix and Exopalaemon carinicauda farmed in the clam–shrimp polyculture system

Unravelling the characterization of hypoxia‐inducible factor‐1α ( HIF‐1α ) and antioxidant enzymes in clam Cyclina sinensis in response to hypoxia

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