Ontogenetic changes in digestive enzyme activity of the spiny lobster, Jasus edwardsii (Decapoda; Palinuridae)

Johnston, Danielle

doi:10.1007/s00227-003-1154-0

Cited by 93 publications

(83 citation statements)

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“…However, because snail meat was the only diet provided for E. sinensis during the entire experimental period, high amylase and cellulose enzyme activities could not related to the present diets, but was rather attributed to the previous secretion before reproductive migration in the river and lakes where adult crabs fed mainly on hydrophytes. Similar to the present results, cellulose activity has been detected in a wide range of aquatic invertebrates whose diets do not normally include substantial amounts of cellulose (Elyakova et al 1981, Johnston 2003, Pavasovic et al 2004. As proposed by Yokoe and Yasumasu (1964), the distribution of cellulose within the animal kingdom was more closely correlated with animal phylogenetic relationships than with differences and similarities in their feeding habits.…”

Section: Discussionsupporting

confidence: 90%

The response of digestive enzyme activity in the mature Chinese mitten crab, <i>Eriocheir sinensis</i> (Decapoda: Brachyura), to gradual increase of salinity

et al. 2013

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SUMMARY: Mature Chinese mitten crabs, Eriocheir sinensis, were exposed to brackish water or seawater as an obligatory part of their reproductive migration. Physiological and biochemical reorganization were needed to adapt them to this migration. To understand the digestive adjustments of Eriocheir sinensis at biochemical level during this transformation from freshwater to seawater, the response of the activity of five digestive enzymes (amylase, cellulase, pepsin, trypsin and lipase) in the hepatopancreas to salinities increasing gradually from 0 (freshwater) to 35 (seawater) was analysed in mature females and males. Digestive enzymes exhibited significantly higher activities in the hepatopancreas of males than those of females, except lipase. In females, amylase, pepsin and trypsin activities began to decrease significantly as the salinity reached 28, and cellulase activity decreased at 35; in males, a considerable decrease in the activity of digestive enzymes, except lipase, was observed at 21 and higher salinities, while an increase was observed at 14. Reduced enzyme activities at elevated salinities suggest that the digestive capacity of crabs for diets becomes weak, and all these digestive enzymes participated in digestive adjustments during osmoregulation. The initial salinity which induced the decrease of enzyme activity was lower in males than in females, indicating that females were more tolerant to elevated salinities than males from the point of digestive biochemical modulation.Keywords: mature crab, digestive enzyme, sexual difference, metabolic regulation, osmoregulation, salinity. RESUMEN: Respuesta de la actividad enzimática digestiva al incRemento gRadual de la salinidad en el cangRejo de shanghai maduRo, EriochEir sinEnsis (decapoda: BRachyuRa). -Se expusieron cangrejos de Shanghai maduros (Eriocheir sinensis) a agua salobre o agua marina obligatoriamente durante la migración reproductora. A fin de que los ejemplares se adaptaran a esta migración, fue preciso proceder a una reorganización fisiológica y bioquímica. Con objeto de estudiar los ajustes digestivos de carácter bioquímico del Eriocheir sinensis durante la transformación del agua dulce en agua marina, se analizó la reacción de la actividad en el hepatopáncreas de cinco enzimas digestivas (amilasa, celulasa, pepsina, tripsina y lipasa) a un aumento gradual de la salinidad, desde 0 ppt (agua dulce) hasta 35 ppt (agua marina), en ejemplares maduros machos y hembras. Las enzimas digestivas mostraron un grado de actividad notablemente mayor en el hepatopán-creas de los machos que en el de las hembras, con excepción de la lipasa. En las hembras, la actividad de la amilasa, la pepsina y la tripsina comenzó a reducirse notablemente cuando la salinidad alcanzó las 28 ppt, mientras que la actividad de la celulasa descendió cuando se alcanzaron las 35 ppt; en el caso de los machos, se observó un descenso muy pronunciado de la actividad enzimática digestiva a partir de las 21 ppt, aunque la actividad aumentó a las 14 ppt. La menor actividad enzimát...

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Section: Discussionsupporting

confidence: 90%

The response of digestive enzyme activity in the mature Chinese mitten crab, <i>Eriocheir sinensis</i> (Decapoda: Brachyura), to gradual increase of salinity

et al. 2013

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“…For some species of lobsters, ontogenetic shifts in diet have been recorded between early and late juvenile stages owing to the different social organisation of these life-history stages (Johnston 2003). We found no evidence of any difference in diet between the lobsters of different size classes (< 80, 80-120 and >120 mm CL) examined here.…”

Section: Lobster Diet: Previous Studiescontrasting

confidence: 51%

Trophic effects of fishing southern rock lobster Jasus edwardsii shown by combined fatty acid and stable isotope analyses

Guest

Frusher²,

Nichols³

et al. 2009

Mar. Ecol. Prog. Ser.

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The southern rock lobster Jasus edwardsii is a commercial species that has benefited from the complete protection offered by no-take reserves, with higher abundances and larger animals recorded in reserves than in adjacent fished areas. What remains unclear is whether there is any change in the diet of lobsters in reserves, for example, as a result of increased intraspecific competition for food. We used combined chemical tracers to examine the diet of lobsters in fished and reserve areas in 2 bioregions in eastern Tasmania. δ 15 N values of lobsters were richer in fished than in reserve areas, indicating that lobsters eat a greater proportion of food items from higher trophic levels in fished areas. Mixing models suggest that ascidians, sea urchins and the turbinid gastropod were all important food sources for lobsters, but the importance of these food items differed between bioregions. This spatial variability may suggest that the small size of the reserve in one bioregion is inadequate at ensuring the diet of lobsters is protected from fishing pressure. Fatty acid profiles of lobsters supported the importance of these food sources to lobsters. Differences between bioregions, or inside and outside of reserves, were not apparent using fatty acids. The present study highlights that lobster fishing has the capacity to alter the trophic status of prey for generalist predators and suggests that fatty acid analyses may be limited in detecting changes in the dietary composition of such generalist feeders. KEY WORDS: Effects of fishing · Food webs · Marine protected areas · Stable isotopes · Fatty acids Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 388: [169][170][171][172][173][174][175][176][177][178][179][180][181][182][183][184] 2009 lishment of marine reserves in which complete cessation of fishing is enforced (Edgar & Barrett 1997, Shears et al. 2006. Increases in the abundance of J. edwardsii have been linked with trophic cascades in reserves that shifted from sea urchin-to algal-dominated reef habitats (Shears & Babcock 2003). What remains unclear, however, is the potential dietary shift of lobsters due, in part, to greater lobster density after reserve establishment (e.g. via intraspecific competition) that may have unknown effects on other components of the reef community.Chemical tracers such as stable isotope and fatty acid analyses are means by which the diet of lobsters in fished and reserve areas may be determined. As lobsters macerate their food upon ingestion, the chemical tracer approach has advantages over conventional methods of dietary analysis, such as gut content analysis where identification is often labour intensive, requires taxonomic expertise and soft bodied organisms may be overlooked (Sheppard & Harwood 2005). Moreover, chemical tracers identify food that is assimilated over a period of time and is of nutritional importance, rather than that which is ingested at one point in time (Thomas & Cahoon 1993). The basis of the chem...

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“…As in most crustacea, trypsin enzymes play a central role in protein digestion in spiny lobsters (Johnston, 2003;Celis-Gerrero et al, 2004;Perera et al, 2008a). Some information is available on the biochemical characterization of spiny lobster trypsins (Galgani and Nagayama, 1987;Celis-Gerrero et al, 2004;Perera et al, 2008a) and variation in trypsin activity throughout development and molt stages has also been reported (Johnston, 2003;Perera et al, 2008b).…”

Section: Introductionmentioning

confidence: 99%

“…Some information is available on the biochemical characterization of spiny lobster trypsins (Galgani and Nagayama, 1987;Celis-Gerrero et al, 2004;Perera et al, 2008a) and variation in trypsin activity throughout development and molt stages has also been reported (Johnston, 2003;Perera et al, 2008b). However, the effect of diet on these enzymes has been poorly studied and the time course of trypsin activity after ingestion of different diets has only recently been reported for one spiny lobster species (Jasus edwardsii) (Simon, 2009).…”

Section: Introductionmentioning

confidence: 99%

Dietary protein quality differentially regulates trypsin enzymes at the secretion and transcription level in Panulirus argus by distinct signaling pathways

Perera

Rodríguez-Viera

Rodríguez-Casariego

et al. 2012

Journal of Experimental Biology

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with the results of previous digestibility studies (Ward et al., 2003; Irving and Williams, 2007;Perera et al., 2010b). Poor protein use could be related to some impairment in digestive progression when lobsters are fed on pelleted diets (Simon and Jeffs, 2008;Simon, 2009).The aim of the present work was to assess the effect of feeding P. argus with different protein sources on trypsin expression and secretion. Our results demonstrate that trypsin enzymes are differentially regulated at the transcription and secretion level by ingested proteins. Additionally, we provide evidence that intact proteins, rather than small peptides or free amino acids, are the major signal eliciting the prandial secretory response in lobster, whereas SUMMARYThe effects of pelleted diets with different protein composition (fish, squid or soybean meals as main protein sources) on trypsin secretion and expression were studied in the lobster Panulirus argus. Trypsin secretion was shown to be maximal 4h after ingestion. At this time, fish-and squid-based diets induced trypsin secretion, as well as up-regulation of the major trypsin isoform at the transcription level. While fish-and squid-based diets elicited a prandial response, soybean-based diet failed to stimulate the digestive gland to secrete trypsin into the gastric fluid or induce trypsin expression above the levels observed in fasting lobsters. In vitro assays showed that intact proteins rather than protein hydrolysates stimulate trypsin secretion in the lobster. However, the signal for trypsin transcription appears to be different to that for secretion and is probably mediated by the appearance of free amino acids in the digestive gland, suggesting a stepwise regulation of trypsin enzymes during digestion. We conclude that trypsin enzymes in P. argus are regulated at the transcription and secretion level by the quality of dietary proteins through two distinct signaling pathways. Our results indicate that protein digestion efficiency in spiny lobsters can be improved by selecting appropriated protein sources. However, other factors like the poor solubility of dietary proteins in dry diets could hamper further enhancement of digestion efficiency.

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Ontogenetic changes in digestive enzyme activity of the spiny lobster, Jasus edwardsii (Decapoda; Palinuridae)

Cited by 93 publications

References 54 publications

The response of digestive enzyme activity in the mature Chinese mitten crab, <i>Eriocheir sinensis</i> (Decapoda: Brachyura), to gradual increase of salinity

The response of digestive enzyme activity in the mature Chinese mitten crab, <i>Eriocheir sinensis</i> (Decapoda: Brachyura), to gradual increase of salinity

Trophic effects of fishing southern rock lobster Jasus edwardsii shown by combined fatty acid and stable isotope analyses

Dietary protein quality differentially regulates trypsin enzymes at the secretion and transcription level in Panulirus argus by distinct signaling pathways

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