Ontogenetic development of neuropeptide Y-like-immunoreactive cells in the gastroenteropancreatic endocrine system of the dogfish

Chibá, Akira; Honma, Yoshio; Oka, Shunya

doi:10.1007/bf00319130

Cited by 11 publications

(6 citation statements)

References 31 publications

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“…Single endocrine cells are known from the digestive tract of cartilaginous fishes (Cimini et al, 1989;Chiba et al, 1995), Polypterus (Zaccone et al, 1995), lungfishes (Tagliafierro et al, 1996), bowfin Amia calva (Youson et al, 2001), gar Lepisosteus osseus (Groff and Youson, 1997), and several species of teleosts (Tagliafierro, 1999).…”

Section: Discussionmentioning

confidence: 99%

Morphology of the air‐breathing stomach of the catfish Hypostomus plecostomus

Podkowa

Goniakowska-Witalińska

2003

Journal of Morphology

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Histological and ultrastructural investigations of the stomach of the catfish Hypostomus plecostomus show that its structure is different from that typical of the stomachs of other teleostean fishes: the wall is thin and transparent, while the mucosal layer is smooth and devoid of folds. The epithelium lining the whole internal surface of the stomach consists of several types of cells, the most prominent being flattened respiratory epithelial cells. There are also two types of gastric gland cells, three types of endocrine cells (EC), and basal cells. The epithelial layer is underlain by capillaries of a diameter ranging from 6.1-13.1 microm. Capillaries are more numerous in the anterior part of the stomach, where the mean number of capillary sections per 100 microm of epithelium length is 4, compared with 3 in the posterior part. The cytoplasm of the epithelial cells, apart from its typical organelles, contains electron-dense and lamellar bodies at different stages of maturation, which form the sites of accumulation of surfactant. Small, electron-dense vesicles containing acidic mucopolysaccharides are found in the apical parts of some respiratory epithelial cells. Numerous gastric glands (2 glands per 100 microm of epithelium length), composed of two types of pyramidal cells, extend from the surface epithelium into the subjacent lamina propria. The gland outlets, as well as the apical cytoplasm of the cells are Alcian blue-positive, indicating the presence of acidic mucopolysaccharides. Zymogen granules have not been found, but the apical parts of cells contain vesicles of variable electron density. The cytoplasm of the gastric gland cells also contains numerous electron-dense and lamellar bodies. Gastric gland cells with electron-dense cytoplasm and tubulovesicular system are probably involved in the production of hydrochloric acid. Fixation with tannic acid as well as with ruthenium red revealed a thin layer of phospholipids and glycosaminoglycans covering the entire inner surface of the stomach. In regions of the epithelium where the capillaries are covered by the thin cytoplasmic sheets of the respiratory epithelial cells, a thin air-blood barrier (0.25-2.02 microm) is formed, thus enabling gaseous exchange. Relatively numerous pores closed by diaphragms are seen in the endothelium lining the apical and lateral parts of the capillaries. Between gastric gland cells, solitary, noninnervated endocrine cells (EC) of three types were found. EC are characterized by lighter cytoplasm than the surrounding cells and they contain dense core vesicles (DCV) with a halo between the electron-dense core and the limiting membrane. EC of type I are the most abundant. They are of an open type, reaching the stomach lumen. The round DCV of this type, with a diameter from 92-194 nm, have a centrally located core surrounded by a narrow halo. EC of type II are rarely observed and are of a closed type. They possess two kinds of DCV with a very narrow halo. The majority of them are round, with a diameter ranging from 88-177 nm, whil...

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Section: Discussionmentioning

confidence: 99%

Morphology of the air‐breathing stomach of the catfish Hypostomus plecostomus

Podkowa

Goniakowska-Witalińska

2003

Journal of Morphology

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“…If this is the sequence of differentiation of larval islet tissue then it would be inconsistent with that observed in many other vertebrates, for PP-family peptides are among the last to appear (Berwert et al, 1995). It is important to extend these observations to embryonic intervals in lampreys, for peptides such as NPY have been shown to appear in embryogenesis of the elasmobranch GEP system (Chiba et al, 1995). The youngest larvae to be examined in lampreys (35 days old) already showed insulin-immunoreactive cell clumps in the intestinal epithelium and submucosal area but also in the extrahepatic common bile duct (Youson, 1999).…”

Section: Agnathansmentioning

confidence: 94%

“…The endocrine cells of the gastrointestinal system of elasmobranchs has received a great deal of attention over the years and the reader is referred to several of the early and recent reports (El-Salhy, 1984;Cimini et al, 1989;Tagliafierro et al, 1989;Yui et al, 1990;Chiba et al, 1995;Chiba, 1998). However, despite the many species of elasmobranchs that have been investigated no phylogenetic pattern involving the presence/ absence or distribution of regulatory peptides in the stomach and intestine has emerged.…”

Section: General Morphology Of the Islet Organ In Chondrichthyesmentioning

confidence: 99%

“…According to the latter authors, the early appearance of these peptides, and others (for example, vasoactive intestinal peptide, Tagliafierro et al, 1988), is support for the view that regulatory peptides play a role in cell growth and differentiation. Recently, there have been extensive studies on the ontogeny of NPY within the GEP system and vitellointestinal duct (VID) of embryos of Scyliorhinus torazame (Chiba et al, 1995;Chiba, 1998). Immunoreactivity for NPY was detected in the sac-like pancreas at the earliest embryonic stage, 15 mm long.…”

Section: Cartilaginous Fishmentioning

confidence: 99%

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Ontogenetic and Phylogenetic Development of the Endocrine Pancreas (Islet Organ) in Fishes

Youson

Al‐Mahrouki

1999

General and Comparative Endocrinology

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The morphology of the gastroenteropancreatic (GEP) system of fish was reviewed with the objective of providing the phylogenetic and ontogenetic development of the system in this vertebrate group, which includes agnathans and gnathostome cartilaginous, actinoptyerygian, and sarcopterygian fish. Particular emphasis is placed on the fish homolog of the endocrine pancreas of other vertebrates, which is referred to as the islet organ. The one-hormone islet organ (B cells) of larval lampreys is the most basic pattern seen among a free-living vertebrate, with the two-hormone islet organ (B and D cells) of hagfish and the three-hormone islet organ (B, D, and F cells) of adult lampreys implying a phylogenetic trend toward the classic four-hormone islet tissue (B, D, F, and A cells) in most other fish. An earlier stage in the development of this phylogenetic sequence in vertebrates may have been the restriction of islet-type hormones to the alimentary canal, like that seen in protochordates. The relationship of the islet organ to exocrine pancreatic tissue, or its equivalent, is variable among bony, cartilaginous, and agnathan fishes and is likely a manifestation of the early divergence of these piscine groups. Variations in pancreatic morphology between individuals of subgroups within both the lamprey and chondrichthyan taxa are consistent with their evolutionary distance. A comparison of the distribution and degree of concentration of the components of the islet organ among teleosts indicates a diffuse distribution of relatively small islets in the generalized euteleosts and the tendency for the concentration into Brockmann bodies of large (principal) islets (with or without secondary islets) in the more derived forms. The holostean actinopterygians (Amiiformes and Semiontiformes) share with the basal teleosts (osteoglossomorphs, elopomorphs) the diffuse arrangement of the components of the islet organ that is seen in generalized euteleosts. Since principal islets are also present in adult lampreys the question arises whether principal islets are a derived or a generalized feature among teleosts. There is a paucity of studies on the ontogeny of the GEP system in fish but it has been noted that the timing of the appearance of the islet cell types parallels the time that they appear during phylogeny; the theory of recapitulation has been revisited. It is stressed that the lamprey life cycle provides a good opportunity for studying the development of the GEP system. There are now several markers of cell differentiation in the mammalian endocrine pancreas which would be useful for investigating the development of the islet organ and cells of the remaining GEP system in fish.

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“…Until now, the regional distribution and relative frequency of numerous types of regulatory peptides have been demonstrated in the gut of the Pisces. Some endocrine cells in the gut of the various species of bony fish (Rombout and Reinecke, 1984; Rombout et al., 1986; Abad et al., 1987; Jonsson et al., 1987; Elbal et al., 1988; Beorlegui et al., 1992; Kiliaan et al., 1992; Pan and Fang, 1993; Himick and Peter, 1994; Tagliafierro et al., 1996; Andreozzi et al., 1997; Groff and Youson, 1997; Reinecke et al., 1997; Kurokawa et al., 2000), cartilaginous fish (Holmgren and Nilsson, 1983; Faraldi et al., 1988; Tagliafierro et al., 1988; Cimini et al., 1992; Chiba et al., 1995) and cyclostomes (Reinecke, 1987; Cheung et al., 1991; Youson and Potter, 1993) were studied by histochemical, electron microscopical and immunohistochemical methods. In addition, Al‐Mahrouki and Youson (1998) reported that immunohistochemical study of the endocrine cells within the gastro‐entero‐pancreatic system of Osteoglossomorpha, an ancient teleostean group, and they detected some different distribution and frequency of endocrine cells between five species of the Osteoglossomorpha.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Study on the Endocrine Cells in Gut of the Stomachless Teleost, Zacco platypus (Cyprinidae)

Lee

2004

Anat Histol Embryol

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The regional distribution and relative frequency of neurohormonal peptides-producing cells were demonstrated in the gut of the stomachless teleost, Zacco platypus (Temminck et Schegel), using 10 types of specific antisera raised against mammalian regulatory peptides. The gut of Z. platypus was divided into five portions from proximal to distal (segment I-V). Most of immunoreactive cells in the epithelial lining portion, between epithelial cells, were generally spherical or spindle shape having long cytoplasmic process that was reached via the lumen while cells round in shape were found in the basal portions of epithelial lining occasionally. Serotonin-, somatostatin-, glucagon-, cholecystokinin (CCK)-8 and pancreatic polypeptide (PP)-immunoreactive cells were observed in this study. However, no chromogranin A-, secretin-, vasoactive intestinal peptide (VIP)-, substance P- and bombesin-immunoreactive cells were found. Serotonin-immunoreactive cells were demonstrated throughout the entire gut tract and occurred more frequently than other cells. Somatostatin-immunoreactive cells were restricted to proximal segments of the gut (segment I-III) with rare frequencies, and glucagon-immunoreactive cells were demonstrated in the proximal segments of the gut (segment I, II) with moderate to few occurrences. CCK-8-immunoreactive cells were found throughout the whole intestinal tract except for most proximal segment (segment I) with moderate to few frequencies and PP-immunoreactive cells were demonstrated in the proximal to middle segments, segment I-III, with a few, rare and rare frequencies, respectively.

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Ontogenetic development of neuropeptide Y-like-immunoreactive cells in the gastroenteropancreatic endocrine system of the dogfish

Cited by 11 publications

References 31 publications

Morphology of the air‐breathing stomach of the catfish Hypostomus plecostomus

Morphology of the air‐breathing stomach of the catfish Hypostomus plecostomus

Ontogenetic and Phylogenetic Development of the Endocrine Pancreas (Islet Organ) in Fishes

Immunohistochemical Study on the Endocrine Cells in Gut of the Stomachless Teleost, Zacco platypus (Cyprinidae)

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