Ontogenetic diel vertical migration of the planktonic copepod Calanus sinicus in the Inland Sea of Japan

Huang, C.; Uye, Shin-ichi; Onb, T.

doi:10.1007/bf00349164

Cited by 11 publications

(1 citation statement)

References 16 publications

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“…The planktonic copepod C. sinicus is the main contributor to zooplankton biomass in the shelf ecosystem of the Northwest Pacific Ocean and has a wide range of distribution, large population size, and prolific fecundity. It shows great geographical diversity in many biological and ecological phenotypes, such as the number of generations, timing of reproduction, vertical distribution, seasonal patterns of abundance, and other life history traits [ 38 – 40 ]. Recently, our marine biodiversity monitoring program revealed that the temporal and spatial distributions of C. sinicus varied obviously in the China Seas, which may be a consequence of global climate warming since this species is a warm-temperate one.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Transcriptome Study to Develop Molecular Resources of the CopepodCalanus sinicusfor Their Potential Ecological Applications

Yang

Sun

Yang

et al. 2014

BioMed Research International

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Calanus sinicus Brodsky (Copepoda, Crustacea) is a dominant zooplanktonic species widely distributed in the margin seas of the Northwest Pacific Ocean. In this study, we utilized an RNA-Seq-based approach to develop molecular resources for C. sinicus. Adult samples were sequenced using the Illumina HiSeq 2000 platform. The sequencing data generated 69,751 contigs from 58.9 million filtered reads. The assembled contigs had an average length of 928.8 bp. Gene annotation allowed the identification of 43,417 unigene hits against the NCBI database. Gene ontology (GO) and KEGG pathway mapping analysis revealed various functional genes related to diverse biological functions and processes. Transcripts potentially involved in stress response and lipid metabolism were identified among these genes. Furthermore, 4,871 microsatellites and 110,137 single nucleotide polymorphisms (SNPs) were identified in the C. sinicus transcriptome sequences. SNP validation by the melting temperature (T m)-shift method suggested that 16 primer pairs amplified target products and showed biallelic polymorphism among 30 individuals. The present work demonstrates the power of Illumina-based RNA-Seq for the rapid development of molecular resources in nonmodel species. The validated SNP set from our study is currently being utilized in an ongoing ecological analysis to support a future study of C. sinicus population genetics.

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Section: Discussionmentioning

confidence: 99%