Ontogenetic profile of Antiviral Mx gene and its role in innate immunity in Mrigal,Cirrhinus mrigala(Hamilton 1822)

Abstract

The aim of this study was to determine the ontogeny of antiviral Mx gene expression in different stages of development of Cirrhinus mrigala (mrigal). For this purpose, three experiments were designed; in the first experiment, Mx expression was observed in eggs, milt, poly I:C treated twitching stage and control twitching stage, and the Mx expression was maximum in eggs. In the second experiment, upregulation of Mx transcript was studied in hatchling stages of mrigal after poly I:C induction by bath treatment, while control hatchlings were studied as such. Hatchlings (both control and treated) were collected at 2-, 4-, 6-and 8-h interval after 24, 48, 72 and 96 h of poly I:C induction. The Mx gene expression showed an increasing trend up to 72 h, where it was highest 3.3-fold higher than the control and then decreased at 96 h where it was threefold higher when compared to the control. In the third experiment, expression of Mx transcript was studied in mrigal fry after intramuscular stimulation with poly I:C for a duration of 28 days. A gradual upregulation of Mx transcript was seen for the experimental period of 28 days. From the study, it is concluded that Mx gene is transmitted from egg and twitching stages and reduced in larvae and fry. Hence, ontogenically, without poly I:C induction, Mx gene is constitutively expressed at early stages indicating the antiviral defences transmitted from parents to offspring in the process of embryonic development. This indicates there is an antiviral Mx defence mechanism playing at early stages of the development for a period of 45 days.

Poly I: C stimulates, Mx transcript and promotes an antiviral state in blood of Labeo catla (Hamilton 1822)

“…The existence of Mx protein in calta was witnnessed with qPCR and immunostaining of the blood cells of catla. The Mx transcript was observed by western blotting of poly I:C stimulated blood cells with a band at 72 kDa in L. catla and C. mrigala (Roy et al, 2017). Similarly, an assumed Mx protein of approximately 76 kDa was reported across the organs of poly I: C treated Atlantic halibut and Atlantic salmon parr (Das et al, 2007;Lockhart et al, 2007).…”

Immunostimulant Bathing Influences the Expression of Immune- and Metabolic-Related Genes in Atlantic Salmon Alevins

“…Poly I:C (mimic of RNA virus) has been widely used to induce the expression of antiviral genes in fish [9,10]. It has been reported that Mrigal carp (Cirrhinus mrigala), bathed for 2 hr in water containing Poly I:C, expressed increased levels of MX transcripts [11]. While Poly I:C is widely used as a TLR3/TRIF pathway agonist [12], it can also induce a type I IFN response through the MDA5/NF-kB signaling pathway in miiuy croaker (Miichthys miiuy) [13].…”

Molecular cloning, GTP recognition mechanism and tissue-specific expression profiling of myxovirus resistance (Mx) protein in Labeo rohita (Hamilton) after Poly I:C induction

“…Interestingly in rainbow trout, maternal transfer of IgM and complement components has been studied by 32 . The ontogenic study of Mx gene expression at diffrent developmental stages have been reported in C. mrigala 33,34 . In fishes, the Mx gene is induced by Type-I Interferon, the main cytokine mediating the innate immune antiviral function.…”