Ontogenetic variation in digestive proteinase activity of larvae and postlarvae of the pink shrimp Farfantepenaeus paulensis (Crustacea: Decapoda: Penaeidae)

Lemos, Daniel; Hernández-Cortés, Martha P.; Navarrete, Andrés; García‐Carreño, Fernando; Phan, Van Ngan

doi:10.1007/s002270050666

Cited by 50 publications

(31 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…those of penaeid shrimps, have demonstrated the appearance of endopeptidases in earlier ontogenetic stages (Fang and Lee 1992;Lemos et al 1999Lemos et al , 2002. Only the eggs and the non-feeding nauplius stages lacked trypsin and chymotrypsin, while the activity of both enzymes peaked in the subsequent protozoeal stages.…”

Section: Discussionmentioning

confidence: 99%

Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla

et al. 2006

View full text Add to dashboard Cite

The early ontogenetic stages of the sub-Antarctic king crab Lithodes santolla were analyzed for the presence and activities of a set of important digestive enzymes. The eggs and non-feeding larvae (zoea I-III, megalopa) showed high activities of esterases, phosphatases, and exopeptidases indicating the enzymatic ability to utilize endogeneous yolk reserves. SDS-PAGE showed a continuous decrease of proteins or proteids in the range of 59-81 kDa during ontogenetic development from the eggs through the zoeal stages to the first juvenile crab stage, CI. This reduction reflects the degradation of storage compounds during lecithotrophic larval development. Activities of the endopeptidases, trypsin and chymotrypsin, were low in eggs and larvae but increased significantly in the first juvenile crab stage. These enzymes typically facilitate the first steps of proteolysis in the extra-cellular spaces of the midgut gland and in the stomach. Their scarcity indicates that the larvae of L. santolla are physiologically not prepared to digest external food. This ability seems to appear first in the CI stage. Extracts of juvenile midgut glands and the gastric fluids of adults showed high activities of a variety of digestive enzymes including phosphatases, carbohydrases, as well as endo-and exopeptidases. High activities of digestive enzymes in adults may compensate for scarce food supply and rate-limiting low temperatures in the predominantly sub-Antarctic habitats of L. santolla.

show abstract

Section: Discussionmentioning

confidence: 99%

Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla

et al. 2006

View full text Add to dashboard Cite

show abstract

“…The cuticle layer lining the epithelial cells in the foregut (Icely & Nott 1992, Ceccaldi 1997, Martin & Chiu 2003 constitutes an important physical barrier that may hinder the passage of infectious WSSV particles to the epithelial cells. The pH and enzymes present in the digestive tract of the shrimp (Lovett & Felder 1990, Talbot & Demers 1993, Lemos et al 1999, Ribeiro & Jones 2000, Gamboa-Delgado et al 2003) may damage infectious viral particles leading to their inactivation. It is likely that only a small proportion of infectious virus inoculated orally actually infects cells, which is why it is necessary to use 10 times more virus to infect shrimp by the oral route compared with i.m.…”

Section: Discussionmentioning

confidence: 99%

Standardized white spot syndrome virus (WSSV) inoculation procedures for intramuscular or oral routes

Escobedo-Bonilla¹,

Audoorn²,

Wille³

et al. 2006

Dis. Aquat. Org.

View full text Add to dashboard Cite

In the past, strategies to control white spot syndrome virus (WSSV) were mostly tested by infectivity trials in vivo using immersion or per os inoculation of undefined WSSV infectious doses, which complicated comparisons between experiments. In this study, the reproducibility of 3 defined doses (10, 30 and 90 shrimp infectious doses 50% endpoint [SID 50 ]) of WSSV was determined in 3 experiments using intramuscular (i.m.) or oral inoculation in specific pathogen-free (SPF) Litopenaeus vannamei. Reproducibility was determined by the time of onset of disease, cumulative mortality, and median lethal time (LT 50 ). By i.m. route, the 3 doses induced disease between 24 and 36 h post inoculation (hpi). Cumulative mortality was 100% at 84 hpi with doses of 30 and 90 SID 50 and 108 hpi with a dose of 10 SID 50 . The LT 50 of the doses 10, 30 and 90 SID 50 were 52, 51 and 49 hpi and were not significantly different (p > 0.05). Shrimp orally inoculated with 10, 30 or 90 SID 50 developed disease between 24 and 36 hpi. Cumulative mortality was 100% at 108 hpi with doses of 30 and 90 SID 50 and 120 hpi with a dose of 10 SID 50 . The LT 50 of 10, 30 and 90 SID 50 were 65, 57 and 50 hpi; these were significantly different from each other (p < 0.05). A dose of 30 SID 50 was selected as the standard for further WSSV challenges by i.m. or oral routes. These standardized inoculation procedures may be applied to other crustacea and WSSV strains in order to achieve comparable results among experiments.

show abstract

“…These ontogenetic events are accompanied by significant changes in metabolic rates and digestive enzyme activities (Lovett & Felder, 1990a;Chu & Ovsianico-Koulikowsky, 1994;Lemos et al, 1999). Current results apparently indicate that different live feed regimes directly influence growth, survival, and trypsin activity level of larvae of Penaeus semisulcatus (table II).…”

Section: Discussionmentioning

confidence: 73%

“…The increasing demand for postlarvae by the shrimp industry and the decreasing availability of postlarvae from the wild has encouraged investigators to concentrate on larval digestive physiology (Kumlu, 1999). Investigations of enzyme response related to ontogenetic variation, feeding, and nutrition have mostly been targeted at shrimp larval stages, e.g., by Lovett & Felder (1990b) for Litopenaeus setiferus (Linnaeus, 1767), by Kamarudin (1992) and Kumlu et al (1992) for Penaeus monodon Fabricius, 1798, by Le Vay et al (1993) and Rodriguez et al (1994) for Marsupenaeus japonicus (Bate, 1888), by Lemos et al (2000) for Litopenaeus schmitti (Burkenroad, 1936), by Kumlu & Jones (1995a) for Fenneropenaeus indicus (H. Milne Edwards, 1837), by Lemos et al (1999) for Farfantepenaeus paulensis (Perez Farfante, 1967), and by Puello-Cruz et al (2002) for Litopenaeus vannamei (Boone, 1931). However, there have been no attempts to study the digestive enzymes of Penaeus semisulcatus to date.…”

Section: Introductionmentioning

confidence: 97%

Trypsin enzyme activity during larval development of Penaeus semisulcatus De Haan, 1844, fed on live feeds

Baysal¹,

Türkmen²

2007

Crustac

View full text Add to dashboard Cite

The growth, survival, and trypsin activity response of Penaeus semisulcatus larvae was examined with two different live feed regimes throughout larval development under laboratory conditions. In the first feeding regime, larvae were fed standard live diets of mixed microalgae from the first to the third protozoea (PZ1 to PZ3), followed by Artemia nauplii until postlarva 1 (PL1). In the second feeding regime, larvae were fed a diet of Artemia nauplii at the beginning of the PZ2 stage. Like other penaeids, P. semisulcatus larvae showed high trypsin activity during the late protozoeal or early mysis stages, which decreased during subsequent stages when fed on conventional live diets of microalgae followed by Artemia nauplii during the mysis stages. P. semisulcatus larvae fed Artemia nauplii from PZ2 onward were significantly heavier on reaching M1 than those fed microalgae (P < 0.05). In contrast, the survival rate of larvae fed with the first feeding regime was higher than that of those in the second feeding regime (P < 0.05). The trypsin activity level in stages PZ2, PZ3, M1, and M2 fed Artemia nauplii was significantly lower than that in the same stages fed microalgae (P < 0.05). RÉSUMÉLa croissance, la survie et l'activité de la trypsine des larves de Penaeus semisulcatus ont été examinées avec deux régimes alimentaires différents au cours du développement larvaire en laboratoire. Dans le premier régime alimentaire les larves ont été nourries avec un régime vivant standard fait d'un mélange de micro-algues au cours des stades prézoés PZ1 à PZ3, suivi par des nauplii d'Artemia jusqu'à la post larve 1 (PL1). Dans le second régime alimentaire, les larves ont été nourries de nauplii d'Artemia à partir du stade PZ2. Comme chez les autres pénéides, les larves de P. semisulcatus montrent une forte activité de la trypsine au cours des derniers stades prézoés et au début des stades mysis, activité qui décroît au cours des stades suivants avec le premier régime. Les larves de P. semisulcatus nourries avec des nauplii d'Artemia à partir de PZ2 ont été significativement plus lourdes en mysis 1 (M1) que celles nourries avec les micro-algues (P < 0,05). Par contre, le taux de survie des larves nourries avec le premier régime a été plus élevé que celui de celles nourries avec le deuxième régime (P < 0,05). Le niveau d'activité de la trypsine au cours des stades PZ2, PZ3, M1 et M2 nourris avec les nauplii d'Artemia a été significativement plus bas que celui des mêmes stades nourris par micro-algues (P < 0,05).

show abstract

Ontogenetic variation in digestive proteinase activity of larvae and postlarvae of the pink shrimp Farfantepenaeus paulensis (Crustacea: Decapoda: Penaeidae)

Cited by 50 publications

References 47 publications

Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla

Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla

Standardized white spot syndrome virus (WSSV) inoculation procedures for intramuscular or oral routes

Trypsin enzyme activity during larval development of Penaeus semisulcatus De Haan, 1844, fed on live feeds

Contact Info

Product

Resources

About