Ontogeny and functional histochemistry of the digestive and visual systems and other organs during the larval development of the thick-lipped grey mullet, &lt;em&gt;Chelon labrosus&lt;/em&gt;

Sarasquete, Carmen; Ortiz-Delgado, Juan B.; Martos-Sitcha, Juan Antonio; Heras, Verónica de las; Yúfera, Manuel; Martı́nez-Rodrı́guez, Gonzalo

doi:10.3989/scimar.04091.27b

Cited by 10 publications

(26 citation statements)

References 53 publications

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“…In this regard, it can be concluded that growth processes in C. labrosus are influenced by stocking density depending on the age of the specimens. In addition, different patterns of body mass increase have been previously demonstrated in larvae and early juveniles of this species from hatching to 99 dph attending to age and developmental ontogenetic changes (Sarasquete et al, 2014). Moreover, growth rates did not show alterations related to the culture conditions in any of the periods analyzed in this experiment, but they are influenced by the age period during which the stress source (stocking density) is applied.…”

Section: Tablementioning

confidence: 74%

Influence of stocking density on growth, metabolism and stress of thick- lipped grey mullet (Chelon labrosus) juveniles

et al. 2015

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Physiological responses to different stocking densities and their subsequent effects on growth rate, energy metabolism and endocrine system were assessed in thick-lipped grey mullet (Chelon labrosus) juveniles. Three different groups of 150 fish (0.383 ± 0.020 g body mass, 101 days post-hatching (dph)) were maintained in triplicate under three different stocking densities: 0.7, 2.0 and 6.7 kg·m −3 . All individuals were sampled at days 0, 20, 45 and 75 to obtain biometric parameters, while 25-30 specimens from each treatment were sampled for the plasma, liver and pituitary collection at the end of the experiment (75 days). The lowest growth increase, both in body mass and total length, was shown in the group held at the highest stocking density (6.7 kg·m −3 ), just by the end of the experiment (176 dph). Moreover, higher plasma cortisol and glucose values were obtained in the group stocked at 0.7 kg·m −3 , whereas individuals maintained under the maximum density (6.7 kg·m −3 ) had the highest hepatic glycogen and lowest glucose content. In addition, growth hormone (GH) and insulin-like growth factor (IGF-I) gene expression increased in the group maintained under the highest stocking condition.Our results indicate that C. labrosus juveniles activated both Hypothalamus-Pituitary-Interrenal (cortisol) and somatotropic (GH/IGF-I) axes to modulate metabolic and stress pathways in specimens held at different stocking densities to compensate their growth rates.

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Section: Tablementioning

confidence: 74%

Influence of stocking density on growth, metabolism and stress of thick- lipped grey mullet (Chelon labrosus) juveniles

et al. 2015

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“…A reduced initial growth in early H. guttulatus life stages is related to the simplicity of their gut anatomy, which develops with age from a short and straight tube into a long and segmented duct. The presence of acidophilic granules and supranuclear vacuoles in the intestine has been described in larvae of many species (Boulhic & Gabaudan, ; Gisbert et al ., ; Hachero‐Cruzado et al ., ; Luizi et al ., ; Park et al ., ; Sarasquete et al ., ; Yúfera et al ., ), resulting from protein pinocytotic uptake and lipid accumulation before stomach development and lipid metabolism becomes functional (Gisbert et al ., ; Izquierdo et al ., ). Protein absorption in larval stages is faster in the posterior intestine.…”

Section: Discussionmentioning

confidence: 97%

“…Oesophageal goblet cells are directly linked to the ability of fish larvae to start exogenous feeding, improving food ingestion. They play an important role lubricating and protecting the digestive mucosa from chemical and physical damages, thus facilitating the transit of food (Sarasquete et al ., ; Sarasquete et al .,). Moreover, goblet cells take part in physiological processes such as nutrient absorption, antimicrobial function, binding of hormones and protection of cells from phagocytosis and dehydration (Bansil & Turner, ; Díaz et al ., ; Domeneghini et al ., ).…”

Section: Discussionmentioning

confidence: 99%

“…The presence of acidophilic granules and supranuclear vacuoles in the intestine has been described in larvae of many species (Boulhic & Gabaudan, 1992;Gisbert et al, 2004;Hachero-Cruzado et al, 2009;Luizi et al, 1999;Park et al, 2015;Sarasquete et al, 2014;Yúfera et al, 2014), resulting from protein pinocytotic uptake and lipid accumulation before stomach development and lipid metabolism becomes functional (Gisbert et al, 1998;Izquierdo et al, 2000). Protein absorption in larval stages is faster in the posterior intestine.…”

Section: Discussionmentioning

confidence: 99%

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Histological development of the long‐snouted seahorse Hippocampus guttulatus during ontogeny

Ofelio

Díaz

Radaelli

et al. 2018

Journal of Fish Biology

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The objective of the present study was to describe histological development of the European long-snouted seahorse Hippocampus guttulatus, to increase understanding of the biology and physiology of the species. Most vital organs were present in juveniles by the time of their release from the male's pouch. Digestive tract specialization occurred at 89 effective day-degrees (D° ), corresponding to 15 days post partum (dpp), with development of the first intestinal loop and mucosal folding. At 118 D° (20 dpp), lipids were being mobilized from the liver and oocytes attained the perinuclear stage. The fovea emerged at 177 D° (30 dpp), contemporaneous with the shift from pelagic to benthic behaviour in juveniles. At this stage, the most interesting feature was the formation of the second intestinal loop. Male gonads were never observed during the study (from 0 to 354 D° ; 0-60 dpp), but the first oogonia were present at 30 D° (5 dpp). In 354 D° (60 dpp) juveniles, oocytes were observed in a cortical alveoli stage, indicating maturity. Low digestive efficiency was observed at early stages, which was due to a poorly developed gastrointestinal tract and an immature digestive tract prior to 89 D° . The present study demonstrates that approximately 89 and 177 D° represent two important transitional stages in the early development of H. guttulatus. At a temperature of approximately 19 ± 1°C and an age of 1 month (177 D° ), main organs were fully functional, suggesting that the adult phenotype was largely established by that age, with females becoming mature at the age of 2 months (354 D° ).

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“…On the other hand, the current archaeal primers could amplify the archaeal 16S rRNA gene from gut contents of only adults. Generally, archaea have been recorded in the guts of adult fishes, as natural fermenters of indigested food remnants, while fingerlings have incomplete gut, and consequently, may lack the favorite conditions for growth of archaea [22]. Other possibility for negative detection of archaea in fingerling guts may refer to the efficiency of the current primers.…”

Section: Resultsmentioning

confidence: 99%

Composition of Eukaryotic and Prokaryotic rRNA Gene Phylotypes in Guts of Adults and Fingerlings of Mugil cephalus, Inhabiting an Egyptian Mediterranean Estuary

Elsaied¹,

Taleb²

2016

J Phylogenetics Evol Biol

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To understand the contribution of gut microflora to the feeding and health of Mugil cephalus in a Mediterranean estuary, we have explored and evaluated the diversity of rRNA genes in gut contents of both of adults and fingerlings. Bulk DNAs were extracted from the fish guts, followed by PCR, cloning and sequencing of eukaryote 18S rRNA gene; bacterial and archaeal 16S rRNA genes. Rarefaction analyses recorded 11, 18 and 13 phylotype groups of rRNA genes for eukaryotes, bacteria and archaea, respectively, in the adult guts. The fingerling guts contained 6 and 11 phylotype groups of rRNA genes for eukartyotes and bacteria, respectively, while lacked archaea. Adult and fingerling guts were dominated by zooplankton-like 18S rRNA gene phylotypes, belonging to those of rotifers, genus Brachionus and copepods, genus Apocyclops. Both of diatoms, genus Cyclotella-and fish-like rRNA gene phylotypes were recorded only in adult guts. Phylotypes, which belonged to uncultured proteobacteria and cyanobacteria, covered most of the bacterial 16S rRNA gene composition of adult guts. Bacteroidetes-like phylotypes characterized the fingerling guts. The study showed the first record of uncultured thaumarchaeote-like 16S rRNA genes in the fish guts, opening a window for exploring thaumarchaeote biological significances in fish metabolism. The fish guts represented a reservoir for geographic distant species and reflected the plankton ecosystem variability at the studied estuary. The pollution in the estuary could shape a fraction of the fish gut microbiota. To our knowledge, this work presented the first complete profile of rRNA gene diversities for all known microbiome domains in fish guts.

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Ontogeny and functional histochemistry of the digestive and visual systems and other organs during the larval development of the thick-lipped grey mullet, <em>Chelon labrosus</em>

Cited by 10 publications

References 53 publications

Influence of stocking density on growth, metabolism and stress of thick- lipped grey mullet (Chelon labrosus) juveniles

Influence of stocking density on growth, metabolism and stress of thick- lipped grey mullet (Chelon labrosus) juveniles

Histological development of the long‐snouted seahorse Hippocampus guttulatus during ontogeny

Composition of Eukaryotic and Prokaryotic rRNA Gene Phylotypes in Guts of Adults and Fingerlings of Mugil cephalus, Inhabiting an Egyptian Mediterranean Estuary

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