1979
DOI: 10.1002/eji.1830091112
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Ontogeny of immunity in amphibians: Changes in antibody repertoires and appearance of adult major histocompatibility antigens in Xenopus

Abstract: Larval Xenopus anti-2,4-dinitrophenyl antibodies of the low-molecular weight type can be analyzed by isoelectric focusing (IEF). Within a clone of genetically identical animals, tadpoles make antibodies whose IEF spectrotypes are shared by most of the individuals. Adults of the same clone also make antibodies of identical spectrotype, but the adult pattern can be very different from the larval one, although both responses are heterogeneous. The larval spectrotypes that one cannot see in a primary adult respons… Show more

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Cited by 76 publications
(29 citation statements)
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“…In the experiment depicted in Figure 1, we used an immunization protocol which, based on our previous experience, gives the maximal affinity maturation. Boosting over an extended period of time does not improve the affinity (Du Pasquier and Haimovich, 1976;Du Pasquier et al, 1979). Aside from considerations of mutation frequency and rate, we have discussed above two additional lines of evidence that the selection for IgH mutations in Xenopus is much less stringent than it is in mammals: the low R/S value and the high GCIAT mutation ratio.…”
Section: Mutations and Affinity Maturationmentioning
confidence: 96%
See 1 more Smart Citation
“…In the experiment depicted in Figure 1, we used an immunization protocol which, based on our previous experience, gives the maximal affinity maturation. Boosting over an extended period of time does not improve the affinity (Du Pasquier and Haimovich, 1976;Du Pasquier et al, 1979). Aside from considerations of mutation frequency and rate, we have discussed above two additional lines of evidence that the selection for IgH mutations in Xenopus is much less stringent than it is in mammals: the low R/S value and the high GCIAT mutation ratio.…”
Section: Mutations and Affinity Maturationmentioning
confidence: 96%
“…Under such circumstances maturation could take a much longer time to be detected. However, even over long immunization periods no major changes in affinity or antibody spectrotypes can be detected (Du Pasquier and Haimovich, 1976;Du Pasquier et al, 1979). There is also a growing amount of evidence that germinal centers play a a prominent role in T cell dependent antibody responses, especially for the selection of mutants in both primary and secondary responses (Jacob et al, 1991; reviewed by Liu et al, 1992).…”
Section: Mutations and Affinity Maturationmentioning
confidence: 99%
“…MHC class II Ag expression during larval life is restricted to thymic epithelium centrally and to B cells and accessory cells in the periphery (Du Pasquier and Flajnik, 1990; Rollins-Smith and Blair, 1990). T-cell proliferation induced by MLR, a typical helper T-cell assay, can be detected between MHC-disparate larval lymphocytes from stage 55–56 (Du Pasquier and Weiss, 1973; Du Pasquier et al, 1979). …”
Section: Early Development Of the Immune Systemmentioning
confidence: 99%
“…For example MLR is impaired from st 58–59 up to approximately 4 to 6 months after metamorphosis completion (Du Pasquier et al, 1979), as well as proliferation induced by mitogens such as PHA (Fig. 3; Rollins-Smith et al, 1984).…”
Section: Remodeling Of the Immune System During Metamorphosismentioning
confidence: 99%
“…The avalanche of the opened questions concerning the function and developmental relationship between various ab T cells (e.g. Th1, Th2, Th17, NKT)-not mentioning cd T cells-was for me overwhelming and thrown me back to the beginnings that is to the questions concerned with the evolutionary and ontogenic origin of lymphocytes and their early developmental stages, the field to which important and well-known contributions were made by Louis du Pasquier (Azumi et al 2003;Chretien et al 1998;Du Pasquier et al 1979), Fritz Melchers (Kudo and Melchers 1987;Sakaguchi and Melchers 1986), Ton Rolink (Ceredig et al 2009;Rolink et al 1991;Rolink et al 1999), and Hans Reimer Rodewald (Rodewald et al 1992;Rodewald et al 1994;Schlenner and Rodewald 2010).…”
Section: Was One Of the Happiest Experience In My Scientific Life Wimentioning
confidence: 99%