Ontogeny of immunoglobulin synthesis production of IgM, IgG and IgA in newborn piglets

Prokešová, L.; Kovářů, F; Jarosková, L; Kostka, J.; Havránek, T; Rejnek, J.

doi:10.1016/s0145-305x(79)80012-9

Cited by 12 publications

(8 citation statements)

References 14 publications

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“…18 Contrary to these results, our previous studies have already ascertained in fetal and newborn GF pigs spontaneous in vitro immunoglobulin production by different lymphatic organs as well as explicit responses to polyclonal B-cell activators. 13,15,19 In this study we showed that a minute amount of serum immunoglobulins of all isotypes is detectable as early as the 44th day of embryonic life and that the level of natural immunoglobulins increases during fetal ontogeny independent of external stimuli. These naturally occurring antibodies, the majority of which were of IgM isotype, were found to react with phylogenetically conserved molecules (myosin, ssDNA, dextran), autoantigens (TRG, FSH, thymocytes) and bacterial components (E. coli O86, tetanic anatoxin).…”

Section: Discussionmentioning

confidence: 57%

Isotype and antibody specificity of spontaneously formed immunoglobulins in pig fetuses and germ‐free piglets: production by CD5⁻ B cells

et al. 1996

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SUMMARYPig fetuses, colostrum-deprived newborns and germ-free (GF) piglets, animals in which B-cell development is not influenced by maternal regulatory factors, were employed to study the occurrence and specificity of natural antibodies (NAb). Serum immunoglobulins of all isotypes were found in 44-day-old fetuses (the gestation period in pigs lasts 114 days) and their level, with predominating IgM, was increased during fetal ontogeny. In sera of fetuses at the end of embryonic life as well as of newborns and older GF piglets, antibody activity against autoantigens (thyroglobulin, hormones, ssDNA), phylogenetically conserved proteins (myosin), haptens (trinitrophenyl; TNP) and bacterial components (Escherichia coli O86, tetanic anatoxin) was detected by enzyme-linked immunosorbent assay. The antigen-binding activity of IgM NAb increased after isolation of the serum immunoglobulins on a Staphylococcus Protein A (SPA)-Sepharose column. IgM reactivity similar to that detected in serum was found in supernatants from polyclonally stimulated cultures of spleen of 8-and 12-day-old GF piglets. Pig fetal liver IgM B cells, which were able to produce IgM after polyclonal stimulation, did not express the CD5 molecule. Our results indicate that pig preimmune repertoire is comparable to that described in humans and mice, although in contrast to these species pig B-1 cells do not express CD5.

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Section: Discussionmentioning

confidence: 57%

Isotype and antibody specificity of spontaneously formed immunoglobulins in pig fetuses and germ‐free piglets: production by CD5⁻ B cells

et al. 1996

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“…Since in other animal species, too, IgM is regarded as the first Ig synthetized during the ontogenic development, its finding in the sera of foetal pigs seems logical. Moreover, there is evidence to indicate that various organs of foetal pigs and colostrum-deprived piglets as well as lymphocytes isolated from these organs can synthetize all three immunoglobulin classes (Chapman 1974;Prokesova et al 1979;Hampl et al 1980). Immunoglobulins have also been demonstrated on the surfaces of circulating lymphocytes in both newborn colostrum-deprived piglets (J aroskova 1977) and foetal pigs (Bins et al 1972).…”

Section: Discussionmentioning

confidence: 99%

“…By the 105th day IgG had become the predominant Ig in all the cases under study except the thymus. Considering the evidence that in newborn piglets lymphocyte numbers responsible for the synthesis of IgG, IgM and IgA are of the same order of magnitude (Prokesova et al 1979), the rise in IgG can be accounted for primarily by enhanced secretory activity of lymphocytes responsible for its production. However, consideration should be given to the fact that some organs of 105-day old foetuses, particularly the liver, spleen and lung, had a higher blood content than those of the younger foetuses and our results may therefore be partly influenced by the presence of circulating IgG.…”

Section: Discussionmentioning

confidence: 99%

Synthesis of Immunoglobulins IgG, IgM and IgA during the Ontogeny of Foetal Pigs

Fränz¹,

Milon²,

Salmon³

1982

Acta Vet. Brno

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Transfer of proteins from dam to foetus has not been demonstrated in animals with the epitheliochorial placenta which include the pig (Sterzl and Silverstein 1967). Foetal pigs are therefore not affected by maternal immunoglobulins during their intra-uterine life. All serum proteins of foetal pigs are produced by the foetuses themselves and their immune response and, consequently, synthesis of immunoglobulins are therefore unaffected by the presence of maternal antibodies. For this reason, the foetal pig is an excellent model for studies of defence mechanisms in both human and veterinary medicine and has been receiving due attention as to the development of immunity.From a number of studies concerned with the incidence of immunoglobulins in foetal pigs and colostrum-deprived piglets (Sterzl et al. 1960; Franek et al.

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“…After intrauterine immunization half of the number of fetuses was kept in uterus without immunization as controls in each sow (26 fetuses immunized and 26 controls). Besides the classical haematological and quantitative cytological technique (KováfiÛ et al 1995), the following methods were used: Immunochemical technique for demonstration of immunoglobulins using monospecific anti-µ, anti-γ or anti-α sera (Proke‰ová et al 1979). Activities of Na + K + -ATPase, Ca 2+ -ATPase, Mg 2+ -ATPase and gammaglutamyltranspeptidase (GGT) were determined in primary and secondary lymphatic organs as a biochemical criterion of cell membrane maturation (KováfiÛ et al 1982;KováfiÛ et al 1992).…”

Section: Methodsmentioning

confidence: 99%

Physiological and Immunological Profiles after Intrauterine Immunization

Kovářů¹,

Kovárů²,

Fišerová³

et al. 2002

Acta Vet. Brno

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Onset of physiological immunocompetence, i.e. ability to respond to antigen by production of specific antibodies without disturbance of the physiological integrity of the organism was studied in pig fetuses. Closed surgery technique was used to expose the fetuses. Flagellin in non-complete adjuvant was used as antigen. Fetuses were immunized on day 54 of gestation, i.e. in the period when T-B dichotomy in secondary lymphatic organs as principal sign of differentiation is already present. Besides the specific immunological response the intrauterine immunization resulted in increased numbers of agranulocytes within secondary lymphatic organs and in circulating blood. No maturation to their plasma cells but increased activity of gamma-glutamyl transpeptidase (GGT) was observed. Increased concentration of total proteins with predominant increase of albumins and gamma globulins along with a decrease of actual amount of alpha-1-fetoprotein was found in blood serum of immunized fetuses. Shift of maturation curves of erythroid and myeloid line to younger developmental forms appeared in haemopoietic organs of these fetuses. In older fetuses immunized on day 74 of gestation, i.e. when a basis of follicular organisation of lymphatic tissue is present, a typical cytological immune reaction "of adult type" was observed. It was characterized by multiplication of lymphocytes of all size categories and their differentiation to plasma cells. Pig fetuses, development of immunocompetence, lymphatic and hemopoietic organsDevelopmental immunology has an ideal experimental model in porcine fetuses because of the character of placentation in this species (placenta epitheliochorialis, diffuse type). This type of placenta not only prevents transfer of immunoglobulins between mother and fetus but it also markedly constrains the non-controllable antigenic stimulation during intrauterine development (·terzl and Silverstein 1967; KováfiÛ et al. 1969). However, developmental immunology is focused not only on the study of spontaneous development of effector systems of immunity but it also takes advantage of possible induction of immune response at particular developmental stages under precisely defined conditions. For this purpose a unique ontogenetic model of development of physiological immunocompetence has been elaborated. It is based on the ability of developing fetuses to respond to specific antigenic stimulus after intrauterine immunization without affecting their basic physiological indices and integrity (KováfiÛ et al. 1971; KováfiÛ and StoÏick˘ 1986;¤eháková et al. 1996).The onset of physiological immunocompetence against sheep erythrocytes (corpuscular antigen) and φX phage 174 (model of viral antigen) on day 70 of gestation has been demonstrated (·terzl and KováfiÛ 1877). The aim of this experiment was to study the antigenic stimulation during an earlier prenatal period (from day 54 of gestation), i.e. during

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Ontogeny of immunoglobulin synthesis production of IgM, IgG and IgA in newborn piglets

Cited by 12 publications

References 14 publications

Isotype and antibody specificity of spontaneously formed immunoglobulins in pig fetuses and germ‐free piglets: production by CD5⁻ B cells

Isotype and antibody specificity of spontaneously formed immunoglobulins in pig fetuses and germ‐free piglets: production by CD5⁻ B cells

Synthesis of Immunoglobulins IgG, IgM and IgA during the Ontogeny of Foetal Pigs

Physiological and Immunological Profiles after Intrauterine Immunization

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Ontogeny of immunoglobulin synthesis production of IgM, IgG and IgA in newborn piglets

Cited by 12 publications

References 14 publications

Isotype and antibody specificity of spontaneously formed immunoglobulins in pig fetuses and germ‐free piglets: production by CD5− B cells

Isotype and antibody specificity of spontaneously formed immunoglobulins in pig fetuses and germ‐free piglets: production by CD5− B cells

Synthesis of Immunoglobulins IgG, IgM and IgA during the Ontogeny of Foetal Pigs

Physiological and Immunological Profiles after Intrauterine Immunization

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Isotype and antibody specificity of spontaneously formed immunoglobulins in pig fetuses and germ‐free piglets: production by CD5⁻ B cells

Isotype and antibody specificity of spontaneously formed immunoglobulins in pig fetuses and germ‐free piglets: production by CD5⁻ B cells