Ontogeny of leptin and its receptor expression in mouse testis during the postnatal period

Herrid, Muren; O’Shea, Tim; McFarlane, James R.

doi:10.1002/mrd.20796

Cited by 43 publications

(34 citation statements)

References 46 publications

(68 reference statements)

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“…In fact, in the rat, leptin was expressed in seminiferous tubules and in the interstitium of the adult gonads (Chen et al, 2008), whereas Ob-R was confined only in the Leydig cells of mature testes (Caprio et al, 2003), but it was absent in immature gonads. In contrast, in the mouse, leptin and its receptor were detected exclusively in germ cells of immature and mature testes (El-Hefnawy et al, 2000;Herrid et al, 2008). Furthermore, in the human adult testes, leptin was found in seminiferous tubules, whereas Ob-R was detected in Leydig cells (Glander et al, 2002;Soyupek et al, 2005;Ishikawa et al, 2007).…”

Section: Discussionmentioning

confidence: 93%

“…In fact, Lep and/or Ob-R were detected in rodent testes (Caprio et al, 2003;El-Hefnawy et al, 2000;Herrid et al, 2008), in human testes and seminal plasma (Glander et al, 2002;Soyupek et al, 2005;Ishikawa et al, 2007), in human sperm (Aquila et al, 2005), in boar sperm (De Ambrogi et al, 2007), and in pig sperm (Aquila et al, 2008). Furthermore, a recent paper reported the effects of leptin treatment, alone or in combination with dietary energy restriction, on different reproductive parameters of male rats (Sirotkin et al, 2008).…”

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confidence: 99%

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Leptin and Its Receptor Are Expressed in the Testis and in the Epididymis of Young and Adult Pigs

Rago

Aquila

Guido

et al. 2009

The Anatomical Record

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Recent studies indicated that leptin, a 16 kDa hormone, is a regulatory signal in human and rodent male reproduction. This work was designed to investigate the expression of leptin and its receptor in testes and epididymides from immature and mature pigs. Immunolocalization revealed that leptin and its receptor were confined only in the interstitial compartment of immature testes, whereas both proteins were detected in Leydig cells and within seminiferous tubules of mature gonads. The immunostaining of epididymal tissues showed that leptin was absent in the epithelial cells of immature pigs but it was present in all the three regions of mature epididymides, although with a minor signal in the cauda. Conversely, leptin receptor was observed in all the epithelial cells of both immature and mature epididymides. Western blot analysis of tissue extracts detected a 16 kDa band for leptin and five/six isoforms, ranging from 120 to 40 kDa, for leptin receptor. In conclusion, this work has identified, for the first time, leptin and leptin receptor in the testis and in the epididymis of the pig showing a differential cell-type expression pattern of the two proteins in young and adult animals. Therefore, our findings suggest a possible involvement of leptin in endocrine or autocrine/ paracrine control of porcine male reproductive structures. Anat Rec, 292:736-745, 2009. V V C 2009 Wiley-Liss, Inc.

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Section: Discussionmentioning

confidence: 93%

mentioning

confidence: 99%

Leptin and Its Receptor Are Expressed in the Testis and in the Epididymis of Young and Adult Pigs

Rago

Aquila

Guido

et al. 2009

The Anatomical Record

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“…35 Besides the hormonal profile, the lack of information about the morphofunctional analysis of the obese Zucker rat testis has propelled us toward this investigation. Along with its primary actions at the hypothalamic-pituitary level, leptin has direct effects on the proliferation, differentiation of germ cells, and modulation of testicular steroidogenesis, using both autocrine and paracrine mechanisms 36 that can utterly affect the reproductive function. Some reports have already showed that the obese Zucker rats present alterations in some reproductive organ weights, 14,15,35 which could be caused by the lack of leptin stimulus 32 ; however, the study presented here is the first to evaluate the testicular histological characteristics and sperm production in the pubertal phase of the Zucker rat as well as its effects on DNA integrity in its sexual maturity.…”

Section: Short Arrows) Surrounded By Sertoli Cells (Long Arrows) In mentioning

confidence: 99%

Reproductive Function of the Male Obese Zucker Rats: Alteration in Sperm Production and Sperm DNA Damage

et al. 2014

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Obesity has been considered a public health issue in many countries and is of increasing concern for authorities over the past 6 years. The Zucker rat is a good experimental model for obesity and diabetes studies due to its metabolic characteristics that are similar to those developed by humans. A total of 12 obese Zucker rats and their lean littermates were killed in pubertal and young adult phases for assessing organ weights (testis and epididymis), testicular histomorphometric and stereological analyses, daily sperm production, and transit time in the epididymis. Sperm integrity was also investigated in the adult animals using the Comet assay. Alterations in organ weights, seminiferous epithelium architecture, sperm production, and transit time were noticed in the pubertal fatty rats. The volume density of the lymphatic space was decreased in both the ages. Adult animals had a significant increase in the extent of damage found in sperm DNA. Our results show for the first time that leptin receptor deficiency compromises sperm production during puberty and that genetic obese Zucker rats have increased sperm DNA fragmentation.

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“…So, it seems leptin mainly acts through endocrine mechanism in cattle. However, recent publications in other species have been reported that leptin also present in some other tissues such as brain and pituitary (Morash et al 1999), mouse testis (Herrid et al 2008), human semineferous tubuli and seminal plasma (Camina et al 2002) and even human spermatozoa (Aquila et al 2005). So, it increases the probability of paracrine and/or autocrine role of leptin, at least in these organs.…”

Section: Introductionmentioning

confidence: 96%

Leptin mRNA expresses in the bull reproductive organ

et al. 2009

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Leptin, a 167-amino acid hormone, is secreted mainly by fat tissue. It has some powerful effects on the regulation of metabolism and reproductive function through endocrine and probably paracrine mechanisms. The contribution rate of leptin function on the male reproductive system is not still clear. Characterization of leptin expression in reproductive organs will suggest that in addition to its endocrine action, leptin has also paracrine/autocrine effects on reproduction. The expression of functional leptin receptor mRNA has been already recognized in testis of rodents, human and cattle. Thus, the aim of the present study was to investigate the presence of leptin mRNA in the bovine testis, because it will be the first step for understanding of its paracrine/autocrine effects on the male reproductive organs in cattle. The present study was the first to showed leptin mRNA expression in the testis of Holstein cattle using reverse transcription and polymerase chain reaction (RT-PCR) analysis. RT-PCR products were amplified with nested PCR using inner leptin primer pairs to emphasis the first results. Besides, bovine beta actin gene was acted as an internal positive control as well as RNA purification marker. Our findings suggest that in addition to its endocrine actions at the hypothalamic-pituitary axis, leptin can has an autocrine and/or paracrine role in bull testicular function.

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Ontogeny of leptin and its receptor expression in mouse testis during the postnatal period

Cited by 43 publications

References 46 publications

Leptin and Its Receptor Are Expressed in the Testis and in the Epididymis of Young and Adult Pigs

Leptin and Its Receptor Are Expressed in the Testis and in the Epididymis of Young and Adult Pigs

Reproductive Function of the Male Obese Zucker Rats: Alteration in Sperm Production and Sperm DNA Damage

Leptin mRNA expresses in the bull reproductive organ

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