Ontogeny of Modulatory Inputs to Motor Networks: Early Established Projection and Progressive Neurotransmitter Acquisition

Feuvre, Yves Le; Fénelon, Valérie S.; Meyrand, Pierre

doi:10.1523/jneurosci.21-04-01313.2001

Cited by 32 publications

(41 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, for the rest of this paper, Crust-MS effects refer to the effects of short-term bath application of Crust-MS. The PS neurons contain Crust-MS, but histamine is also present, as shown previously (Mulloney and Hall, 1991;Le Feuvre et al, 2001). We therefore examined the effects of a 3ms focal application of histamine to the STG and compared it with the modulation produced by Crust-MS application.…”

Section: Ps Stimulation Crust-ms Application and Focal Histamine Appmentioning

confidence: 76%

“…The pyloric suppressor (PS) neurons of homarid lobsters contain at least two co-transmitters: histamine (Mulloney and Hall, 1991;Le Feuvre et al, 2001) and an FMRFamide-like peptide (Fénelon et al, 1998). Their morphology is well characterized, with terminal endings in all four ganglia of the STNS [the oesophageal ganglion (OG), paired commissural ganglia (CoGs) and stomatogastric ganglion (STG)] (Meyrand et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Coordination of distinct but interacting rhythmic motor programs by a modulatory projection neuron using different co-transmitters in different ganglia

Kwiatkowski

Gabranski

Huber

et al. 2013

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARYWhile many neurons are known to contain multiple neurotransmitters, the specific roles played by each co-transmitter within a neuron are often poorly understood. Here, we investigated the roles of the co-transmitters of the pyloric suppressor (PS) neurons, which are located in the stomatogastric nervous system (STNS) of the lobster Homarus americanus. The PS neurons are known to contain histamine; using RT-PCR, we identified a second co-transmitter as the FMRFamide-like peptide crustacean myosuppressin (Crust-MS). The modulatory effects of Crust-MS application on the gastric mill and pyloric patterns, generated in the stomatogastric ganglion (STG), closely resembled those recorded following extracellular PS neuron stimulation. To determine whether histamine plays a role in mediating the effects of the PS neurons in the STG, we bath-applied histamine receptor antagonists to the ganglion. In the presence of the antagonists, the histamine response was blocked, but Crust-MS application and PS stimulation continued to modulate the gastric and pyloric patterns, suggesting that PS effects in the STG are mediated largely by Crust-MS. PS neuron stimulation also excited the oesophageal rhythm, produced in the commissural ganglia (CoGs) of the STNS. Application of histamine, but not Crust-MS, to the CoGs mimicked this effect. Histamine receptor antagonists blocked the ability of both histamine and PS stimulation to excite the oesophageal rhythm, providing strong evidence that the PS neurons use histamine in the CoGs to exert their effects. Overall, our data suggest that the PS neurons differentially utilize their cotransmitters in spatially distinct locations to coordinate the activity of three independent networks.

show abstract

Section: Ps Stimulation Crust-ms Application and Focal Histamine Appmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Coordination of distinct but interacting rhythmic motor programs by a modulatory projection neuron using different co-transmitters in different ganglia

Kwiatkowski

Gabranski

Huber

et al. 2013

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…However, to contrast this situation with other neurohormones that are expressed during embryonic development, we have also included an analysis of RPCH and PDH, since these are also important neuromodulators. In view of the observations on expression of neuropeptides in the stomatogastric ganglion of lobsters, suggesting that some projection neurones do not express a 'peptide phenotype' until late in development (Le Feuvre et al, 2001), we considered our studies, which involve quantitative measurement of specific mRNAs in conjunction with neuroanatomical studies, to be especially relevant: Do transcript phenotypes mirror peptide expression? For MIH and CHH we observed significant expression of both mRNAs at the beginning of eye anlage formation, which rapidly increased during subsequent development.…”

Section: Discussionmentioning

confidence: 99%

Expression and release patterns of neuropeptides during embryonic development and hatching of the green shore crab, Carcinus maenas

Chung

Webster

2004

Development

View full text Add to dashboard Cite

Crustacean ecdysis is controlled by at least three neuropeptides:moult-inhibiting hormone (MIH), which represses ecdysteroid synthesis;crustacean hyperglycaemic hormone (CHH), which not only influences ecdysteroid synthesis but also water uptake during moulting; and crustacean cardioactive peptide (CCAP), which is involved in stereotyped ecdysis behaviour. During embryonic development, moulting takes place in the egg, but there is little information regarding developmental expression of these neuropeptides during this period or during hatching – an event that is analogous to eclosion in insects. To address this problem, we determined expression profiles of MIH and CHH mRNA by quantitative RT-PCR, together with developmental peptide expression studies [confocal immunocytochemistry (ICC) and radioimmunoassay(RIA)]. Likely homologous events relating to neuropeptide surges of both CHH and CCAP were seen during larval hatching, when compared to the adult moult,and cell-specific copy concentration of both MIH and CHH mRNAs was identical to that of the adult during late embryonic development. We measured parallel mRNA and peptide expression of two neuropeptides (red pigment-concentrating hormone RPCH) and pigment-dispersing hormone (PDH) during development, as these have roles as neuromodulators and as classical neurohormonal roles. For MIH and CHH, gene expression was in accordance with peptide expression, but novel sites of CHH expression were found (abdominal peripheral neurones), the expression and release patterns of which may be related to larval eclosion and water uptake necessary for eggshell rupture and hatching. For RPCH and PDH,gene transcription and peptide expression were not in accordance. A significant contribution of maternally derived (non-translated) PDH mRNA to the embryo was seen, and for RPCH, high-level mRNA and peptide expression during late embryogenesis is related to a long ignored, but potentially important release site – the enigmatic post-commissural organs –which are the most prominent structures expressing RPCH during late embryogenesis.

show abstract

“…Adult, larval, and embryonic STNS were dissected as described previously (Casasnovas and Meyrand, 1995;Le Feuvre et al, 2001).…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies have shown that some of these modulatory GABAergic neurons, in addition to exogenous GABA, can exert important effects on the adult STNS networks (Cazalets et al, 1987;Nagy et al, 1994;Faumont, 1999;Meyrand et al, 2000;Swensen et al, 2000). A recent developmental study has shown that these modulatory neurons are present in the embryo but at least four of them do not express GABA at this developmental stage (Le Feuvre et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Removal of GABA within Adult Modulatory Systems Alters Electrical Coupling and Allows Expression of an Embryonic-Like Network

Ducret¹,

Feuvre²,

Meyrand³

et al. 2007

J. Neurosci.

View full text Add to dashboard Cite

The maturation and operation of neural networks are known to depend on modulatory neurons. However, whether similar mechanisms may control both adult and developmental plasticity remains poorly investigated. To examine this issue, we have used the lobster stomatogastric nervous system (STNS) to investigate the ontogeny and role of GABAergic modulatory neurons projecting to small pattern generating networks. Using immunocytochemistry, we found that modulatory input neurons to the stomatogastric ganglion (STG) express GABA only after metamorphosis, a time that coincides with the developmental switch from a single to multiple pattern generating networks within the STNS. We demonstrate that blocking GABA synthesis with 3-mercapto-propionic acid within the adult modulatory neurons results in the reconfiguration of the distinct STG networks into a single network that generates a unified embryoniclike motor pattern. Using dye-coupling experiments, we also found that gap-junctional coupling is greater in embryos and GABAdeprived adults exhibiting the unified motor pattern compared with control adults. Furthermore, GABA was found to diminish directly the extent and strength of electrical coupling within adult STG networks. Together, these observations suggest the acquisition of a GABAergic phenotype by modulatory neurons after metamorphosis may induce the reconfiguration of the single embryonic network into multiple adult networks by directly decreasing electrical coupling. The findings also suggest that adult neural networks retain the ability to express typical embryonic characteristics, indicating that network ontogeny can be reversed and that changes in electrical coupling during development may allow the segregation of multiple distinct functional networks from a single large embryonic network.

show abstract

Ontogeny of Modulatory Inputs to Motor Networks: Early Established Projection and Progressive Neurotransmitter Acquisition

Cited by 32 publications

References 62 publications

Coordination of distinct but interacting rhythmic motor programs by a modulatory projection neuron using different co-transmitters in different ganglia

Coordination of distinct but interacting rhythmic motor programs by a modulatory projection neuron using different co-transmitters in different ganglia

Expression and release patterns of neuropeptides during embryonic development and hatching of the green shore crab, Carcinus maenas

Removal of GABA within Adult Modulatory Systems Alters Electrical Coupling and Allows Expression of an Embryonic-Like Network

Contact Info

Product

Resources

About