Ontogeny of Osmoregulation in the Crayfish<i>Astacus leptodactylus</i>

Susanto, Gregorius Nugroho; Charmantier, Guy

doi:10.1086/316736

Cited by 30 publications

(27 citation statements)

References 39 publications

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“…Urine is thus markedly hypotonic to hemolymph. The hemolymph osmolality measured is close to previous values reported in the same species (400-450 mosm/kg; Susanto and Charmantier 2000) and lies in the range of values reported in adults of different species of crayfish (366-512 mosm/kg; Bryan Kamemoto et al 1966;Kerley and Pritchard 1967;Andrews 1967;Kamemoto and Ono 1968;Riegel 1968;Sharma 1968;Pritchard and Kerley 1970;Wong and Freeman 1976;Mills and Geddes 1980;Newsom and Davis 1994;Holdich et al 1997). …”

Section: Osmotic and Ionic Regulationsupporting

confidence: 88%

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Ontogeny of the antennal glands in the crayfish Astacus leptodactylus (Crustacea, Decapoda): immunolocalization of Na+,K+-ATPase

Khodabandeh

Kutnik²,

Aujoulat

et al. 2004

Cell Tissue Res

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The involvement of the antennal urinary glands in the ontogeny of osmoregulatory functions was investigated during the development of Astacus leptodactylus by measurements of hemolymph and urine osmolality in juvenile and adult crayfish and by the immunodetection of the enzyme Na+,K+-ATPase. In stage II juveniles, 1-year-old juveniles, and adults, all of which were maintained in freshwater, urine was significantly hypotonic to hemolymph. In adults, chloride and sodium concentrations were much lower in urine than in hemolymph. During embryonic development, Na+,K+-ATPase was detected by immunocytochemistry in ionocytes lining the tubule and the bladder, at an eye index (EI) of 220-250 microm, and in the labyrinth, at EI 350 microm. In all regions, immunofluorescence was mainly located at the basolateral side of the cells. No immunofluorescence was detected at any stage in the coelomosac. In late embryonic stages (EI 410-440 microm), in stage I juveniles, and in adults, strong positive immunofluorescence was found from the labyrinth up to and including the bladder. These results show that, as early as hatching, juvenile crayfish are able to produce dilute urine hypotonic to hemolymph. This ability originates from the presence of Na+,K+-ATPase in ion-transporting cells located in the labyrinth, the tubule, and the bladder of the antennal glands and constitutes one of the main adaptations of crayfish to freshwater.

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Section: Osmotic and Ionic Regulationsupporting

confidence: 88%

“…In crustaceans, this antiserum was previously used in Porcellio scaber (Ziegler 1997) and in Homarus gammarus (Lignot and Charmantier 1999;Lignot et al 2001) and for the study of the gills in A. leptodactylus (Susanto 2000). The methods of preparation and fixation of the specimens were as described in Khodabandeh et al (2004).…”

Section: Immunocytochemistrymentioning

confidence: 99%

Ontogeny of the antennal glands in the crayfish Astacus leptodactylus (Crustacea, Decapoda): immunolocalization of Na+,K+-ATPase

Khodabandeh

Kutnik²,

Aujoulat

et al. 2004

Cell Tissue Res

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“…This relationship has been shown in the isopod Sphaeroma serratum (Charmantier & Charmantier-Daures 1994), in the amphipods Gammarus duebeni (Morritt & Spicer 1995) and Orchestia gammarellus (Morritt & Spicer 1996, 1999, and in the decapods Armases miersii (Charmantier et al 1998), Sesarma curacaoense (Anger & Charmantier 2000), Palaemonetes argentinus (Charmantier & Anger 1999) and Astacus leptodactylus (Susanto & Charmantier 2000). These species all exhibit a retention strategy, i.e.…”

Section: Introductionmentioning

confidence: 82%

Ontogeny of osmoregulation, physiological plasticity and larval export strategy in the grapsid crab Chasmagnathus granulata (Crustacea, Decapoda)

Charmantier

Giménez²,

Charmantier‐Daures³

et al. 2002

Mar. Ecol. Prog. Ser.

109

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The grapsid crab Chasmagnathus granulata populates brackish-water lagoons and other estuarine environments. In its reproduction, this species follows a strategy of larval export, i.e. its larvae live under different salinity conditions from the juveniles and adults. In the present experimental investigation, ontogenetic changes in the capability for osmoregulation were studied in all 4 zoeal stages, the megalopa, the juvenile crab instars I, II and IV, and adults (all reared in seawater, 32 ‰). Moreover, we studied effects of embryonic and larval acclimation on osmoregulation. The zoea I larvae were slight hyper-regulators at low salinities (10 to 17 ‰) and hyper-osmoconformers at higher salinities. Stages II to IV zoeae were generally hyper-osmoconformers. At metamorphosis to the megalopa, the type of osmoregulation changed to hyper-hypo-regulation. The osmoregulatory capacities under both hypo-and hypersaline conditions increased strongly in the crab I and throughout later juvenile development. These patterns in osmoregulation match the ontogenetic changes that typically occur in the ecology of C. granulata: the zoea I hatches in brackish estuarine waters, where the juveniles and adults live, before it is exported to coastal marine zones. This initial larval stage is euryhaline and capable of hyper-osmoregulation at low salinities. The same capabilities were observed in the megalopa, which re-invades the brackish adult environment. This stage is known to settle in semiterrestrial habitats near the adult burrows, where both brackish and hypersaline conditions are likely to occur; this coincides with the first ontogenetic appearance of the hyper-hypoosmoregulation pattern. The zoeal stages II, III and IV, in contrast, develop in the adjacent sea, where the salinity is higher and more stable. Correspondingly, these intermediate larval stages were found to be stenohaline osmoconformers. Preceding exposure of the eggs and larvae to a reduced salinity (20 ‰) enhanced the hyper-osmoregulatory capacity at low salinities (5 to 10 ‰) in all zoeal stages. This indicates an effect of non-genetic acclimation and, hence, phenotypic plasticity. This trait should have an adaptive value, as it increases the chance of larval survival, at least in the initial larval stage, which is in the field exposed to highly variable, mostly reduced salinities.

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“…at the time when they become fully able to hyper-osmoregulate in freshwater. Later in development, the Na + , K + -ATPase is expressed or an increasing number of the gill filaments of juveniles and the capacity to hyper-osmoregulate increases concomitantly (Susanto and Charmantier, 2000).…”

Section: Discussionmentioning

confidence: 99%

Immunolocalization of Na+,K+-ATPase in the branchial cavity during the early development of the crayfish Astacus leptodactylus (Crustacea, Decapoda)

et al. 2004

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The ontogeny of osmoregulation was examined in the branchial cavity of embryonic and early postembryonic stages of the crayfish Astacus leptodactylus maintained in freshwater, at the sub-cellular level through the detection of the Na + ,K + -ATPase. The embryonic rate of development was calculated according to the eye index (EI) which was 430-450 µm at hatching. The distribution of the enzyme was identified by immunofluorescence microscopy using a monoclonal antibody IgGα 5 raised against the avian α-subunit of the Na + ,K + -ATPase.Immunoreactivity staining indicating the presence of Na + ,K + -ATPase appeared in the gills of late embryos (EI ≥ 400 µm), i.e. a few days before hatching time, and steadily increased throughout the late embryonic and early postembryonic development. The appearance of the enzyme correlates with the ability to osmoregulate which also occurs late in the embryonic development at EI 410-420 µm and with tissue differentiation within the gill filaments. These observations indicate that the physiological shift from osmoconforming embryos to hyperregulating late embryos and post-hatching stages in freshwater must originate partly from the differentiation in the gill epithelia of ionocytes which are the site of ion pumping, as suggested by the location of Na + ,K + -ATPase. Only the gills were immunostained and a lack of specific staining was noted in the lamina and the branchiostegites. Therefore, osmoregulation through Na + active uptake is likely achieved in embryos at the gill level; all the newly-formed gills in embryos function in ion regulation; other parts of the branchial chamber such as the branchiostegites and lamina do not appear to be involved in osmoregulation.2

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Ontogeny of Osmoregulation in the CrayfishAstacus leptodactylus

Cited by 30 publications

References 39 publications

Ontogeny of the antennal glands in the crayfish Astacus leptodactylus (Crustacea, Decapoda): immunolocalization of Na+,K+-ATPase

Ontogeny of the antennal glands in the crayfish Astacus leptodactylus (Crustacea, Decapoda): immunolocalization of Na+,K+-ATPase

Ontogeny of osmoregulation, physiological plasticity and larval export strategy in the grapsid crab Chasmagnathus granulata (Crustacea, Decapoda)

Immunolocalization of Na+,K+-ATPase in the branchial cavity during the early development of the crayfish Astacus leptodactylus (Crustacea, Decapoda)

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