1990
DOI: 10.3109/01485019008987582
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Ontogeny of Rat Testicular Mitogenic Activity Studied with Transformed and Nontransformed Mouse 3T3 Fibroblasts

Abstract: The ontogeny of the testicular mitogenic activity of Sprague Dawley rats was evaluated by stimulation in vitro of [3H]thymidine uptake and cell number in Swiss 3T3 and transformed NIH 3T3 fibroblasts. Addition of various amounts of testicular cytosolic protein resulted in a dose-dependent increase in cell number in both cell lines. The [3H]thymidine responses to testicular cytosolic proteins was also dose dependent but in a nonparametric manner. In addition, the responses of 3T3 cells to testicular cytosol was… Show more

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