Ontogeny of Rat Thymic Epithelium Defined by Monoclonal Anticytokeratin Antibodies

Čolić, Miodrag; Jovanović, Suzana; Vasiljevski, Milijana; Dujić, A

doi:10.1155/1990/16952

Cited by 10 publications

(6 citation statements)

References 12 publications

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“…Moreover, immunocytochemical studies have also demonstrated the phenotypical diversity of epithelial cells of the murine, human and rat thymus (van Vliet et al 1984;De Maagd et al 1985;Kampinga et al 1987). Especially notable are the data obtained using the anti-cytokeratin monoclonal antibodies, which demonstrated that there are several different subsets of medullary epithelial cells 1990;Milićević et al 1992). Likewise, various monoclonal antibodies to the medullary epithelial subset react with only some of these cells (Jambon et al 1981;Savino et al 1982;Gaudecker et al 1986;Farr and Anderson 1985;Rouse et al 1988) and, in accordance with our findings, also demonstrate their heterogeneity.…”

Section: Discussionsupporting

confidence: 89%

“…Their findings were confirmed by Gaudecker (1991). However, using anti-cytokeratin antibodies it is possible to distinguish additional subsets of thymic epithelial cells: in the medulla there are more than just two , 1990Milićević et al 1992). Light-microscopic immunophenotyping, using different monoclonal antibodies, has also documented the heterogeneity of epithelial cells in mouse, human and rat thymus, distinguishing between subcapsular, cortical and medullary areas (van Vliet et al 1984;De Maagd et al 1985;Kampinga et al 1987).…”

Section: Introductionmentioning

confidence: 87%

“…Light-microscopic immunophenotyping, using different monoclonal antibodies, has also documented the heterogeneity of epithelial cells in mouse, human and rat thymus, distinguishing between subcapsular, cortical and medullary areas (van Vliet et al 1984;De Maagd et al 1985;Kampinga et al 1987). However, many of the monoclonals directed against different epitopes expressed on medullary epithelial cells (Farr and Anderson 1985;Rouse et al 1988), including the already-mentioned anti-cytokeratin antibodies , 1990Milićević et al 1992), in different patterns stain only a proportion of these cells in the thymic medulla. Especially often, the antibodies against thymic hormones do not react with all of them (Jambon et al 1981;Savino et al 1982;Gaudecker et al 1986).…”

Section: Introductionmentioning

confidence: 99%

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Ultrastructure of different types of thymic epithelial cells in normal and cyclosporin-A-treated rats

Milićević¹,

Milićević²

1997

Anatomy and Embryology

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Transmission electron microscopy was used to study the ultrastructural features of thymic epithelial cells of intact and cyclosporin-A-treated adult male Wistar rats. The animals received a peroral daily dose of 30 mg of cyclosporin A per kg body weight for 21 consecutive days. On the basis of ultrastructural features, seven subsets of epithelial cells were distinguished within the intact rat thymus. Four subsets were predominantly situated in the cortex (type 4 - "dark" cells - occasionally penetrated into the medullar region) and three were positioned within the thymic medulla. All subsets of thymic epithelial cells were markedly changed after the application of cyclosporin A. The prominent enlargement of cytokeratin contents was registered in type 1 - "subcapsular/paraseptal/perivascular" - epithelial cells. At the same time these cells acquired the morpho-functional aspect of resting cells. All other subsets of cortical epithelial cells were enlarged (though in different ways) in comparison to the corresponding subsets of the control thymus, whereby their subcellular organization was suggestive of increased synthetic and secretory activity. The decrease in the number of epithelial cells, with the prevalence of phenotypically immature subsets, was evident within the residual islands of thymic medullary tissue.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

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Ultrastructure of different types of thymic epithelial cells in normal and cyclosporin-A-treated rats

Milićević¹,

Milićević²

1997

Anatomy and Embryology

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“…The cortical region directly under the capsule is called the subcapsular zone (SCZ), where proliferating DN thymocytes are localized ( 25 ). The SCZ contains a unique type of TECs ( 26 , 27 ), but their function has been poorly elucidated. The cortex of the adult mouse thymus contains a network of cTECs (estimated as comprising ~10 6 cells per mouse) that houses densely packed DP thymocytes (more than 10 8 cells per mouse) ( 28 ).…”

Section: Overview Of Thymic Stromal Cellsmentioning

confidence: 99%

Non-Epithelial Thymic Stromal Cells: Unsung Heroes in Thymus Organogenesis and T Cell Development

Nitta

Takayanagi

2021

Front. Immunol.

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The stromal microenvironment in the thymus is essential for generating a functional T cell repertoire. Thymic epithelial cells (TECs) are numerically and phenotypically one of the most prominent stromal cell types in the thymus, and have been recognized as one of most unusual cell types in the body by virtue of their unique functions in the course of the positive and negative selection of developing T cells. In addition to TECs, there are other stromal cell types of mesenchymal origin, such as fibroblasts and endothelial cells. These mesenchymal stromal cells are not only components of the parenchymal and vascular architecture, but also have a pivotal role in controlling TEC development, although their functions have been less extensively explored than TECs. Here, we review both the historical studies on and recent advances in our understanding of the contribution of such non-TEC stromal cells to thymic organogenesis and T cell development. In particular, we highlight the recently discovered functional effect of thymic fibroblasts on T cell repertoire selection.

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“…The subcapsular/subtrabecular TECs link K1, K7, and K19 monoclonal anti-keratin antibodies, bound in a subcapsular/subtrabecular flat layer [72]. This subset of TECs has positive immunoreactivity for tyrosine hydroxylase (TH), α 1 -adrenoceptor (α 1 -AR) (both for TECs and the macrophages subcapsular/subtrabecular located), calcitonin gene-related peptide (CGRP), and bombesin [73,74].…”

Section: Thymic Epithelial Cells (Tecs)mentioning

confidence: 99%

Untitled

2022

Rom J Morphol Embryol

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This article focuses on the latest histological knowledge in the field regarding the peripheral lymphoid system [mucosa-associated lymphoid tissue (MALT), bronchus-associated lymphoid tissue (BALT), gut-associated lymphoid tissue (GALT)], the thymus stroma, some of the various corpuscles of the human body (Hassall's corpuscles in thymus, arenaceous corpuscles in pineal gland, corpora amylacea in prostate and other locations) and Fañanas glial cells in the cerebellum.

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Ontogeny of Rat Thymic Epithelium Defined by Monoclonal Anticytokeratin Antibodies

Abstract: Ontogenetic study on the expression of cytokeratin (CK) polypeptides within particular subsets of rat thymic epithelial cells (TEC)

Cited by 10 publications

References 12 publications

Ultrastructure of different types of thymic epithelial cells in normal and cyclosporin-A-treated rats

Ultrastructure of different types of thymic epithelial cells in normal and cyclosporin-A-treated rats

Non-Epithelial Thymic Stromal Cells: Unsung Heroes in Thymus Organogenesis and T Cell Development

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