Ontogeny of the H19 Gene in Sheep and Effect of Maternal Fasting on Its Expression in the Fetus

Naimeh, Laudy G.; Schutte, Brian C.; Hamilton, Wendy S.; Tsalikian, Eva

doi:10.1081/erc-100107866

Cited by 8 publications

(6 citation statements)

References 37 publications

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“…Tongue RNA from the control animals was also analysed. H19 was expressed at high levels in the tongue of all the lambs, confirming earlier reports on high expression in neonates (Naimeh et al, 2001;Lee et al, 2002), but levels appeared unaltered in the cloned lambs. IGF2 expression was relatively high and appeared to be similar in cloned and control animals.…”

Section: Analysis Of H19 and Igf2r In Lambs Derived By Nuclear Transfersupporting

confidence: 91%

“…The sheep H19 gene was also found to have the same exon -intron structure as in primates and rodents. The conservation of the H19 gene amongst different mammalian groups, and its similar pattern of expression during ovine and murine development (Sasaki et al, 1995;Naimeh et al, 2001;Lee et al, 2002), suggests that the non-coding H19 RNA is functional (Hurst and Smith, 1999). Although H19 RNA seems not to have an essential role in mouse development (Jones et al, 1998), several studies indicate that it might function in regulating the translation of other, coding RNAs, possibly even that of IGF2 (Li et al, 1998;Runge et al, 2000).…”

Section: Discussionmentioning

confidence: 94%

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Conservation of IGF2-H19 and IGF2R imprinting in sheep: effects of somatic cell nuclear transfer

Young

Schnieke

McCreath

et al. 2003

Mechanisms of Development

112

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In different mammalian species, in vitro culture and manipulation can lead to aberrant fetal and peri-natal development. It has been postulated that these diverse abnormalities are caused by epigenetic alterations and that these could affect genes that are regulated by genomic imprinting. To explore this hypothesis relative to somatic cell nuclear transfer in sheep, we investigated whether the ovine H19-IGF2 and IGF2R loci are imprinted and analysed their DNA methylation status in cloned lambs. A comparison between parthenogenetic and control concepti established that imprinting at these two growth-related loci is evolutionarily conserved in sheep. As in humans and mice, IGF2R and H19 comprise differentially methylated regions (DMRs) that are methylated on one of the two parental alleles predominantly. In tongue tissue from 12 out of 13 cloned lambs analysed, the DMR in the second intron of IGF2R had strongly reduced levels of DNA methylation. The DMR located upstream of the ovine H19 gene was found to be similarly organised as in humans and mice, with multiple CTCF binding sites. At this DMR, however, aberrant methylation was observed in only one of the cloned lambs. Although the underlying mechanisms remain to be determined, our data indicate that somatic cell nuclear transfer procedures can lead to epigenetic deregulation at imprinted loci.

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Section: Analysis Of H19 and Igf2r In Lambs Derived By Nuclear Transfersupporting

confidence: 91%

Section: Discussionmentioning

confidence: 94%

Conservation of IGF2-H19 and IGF2R imprinting in sheep: effects of somatic cell nuclear transfer

Young

Schnieke

McCreath

et al. 2003

Mechanisms of Development

112

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“…Fetuses from mouse embryos cultured in the presence of serum had 31 and 15% reduction in H19 and Igf2 respectively compared with in vivo counterparts (Khosla et al 2001b). In addition, fetal liver from pregnant ewes fasted for 48 h showed a 61% reduction in H19 without any alteration in IGF2 expression (Naimeh et al 2001). It is possible that the expression of H19 and Igf2 are regulated independently under certain circumstances.…”

Section: Discussionmentioning

confidence: 99%

“…The level of IGF2 protein can also be regulated by IGF2R (Lau et al 1994). Previously, studies have shown that H19 is very sensitive to preimplantation culture condition (Doherty et al 2000, Lane & Gardner 2003, Mann et al 2004) and maternal dietary restriction altered H19 and IGF2 gene expression in fetal sheep (Brameld et al 2000, Naimeh et al 2001. The culturing of sheep embryos to blastocyst stage led to reduction in IGF2R transcript and protein in late gestation (Young et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Imprinted gene expression in the rat embryo–fetal axis is altered in response to periconceptional maternal low protein diet

Kwong¹,

Miller²,

Ursell³

et al. 2006

Reproduction

119

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In our previous study, we have shown that maternal low protein diet (LPD, 9% casein vs 18% casein control) fed exclusively during the rat preimplantation period (0-4.25 day postcoitum) induced low birth weight, altered postnatal growth and hypertension in a gender-specific manner. In this study, we investigated the effect of maternal LPD restricted only to the preimplantation period (switched diet) or provided throughout gestation on fetal growth and imprinted gene expression in blastocyst and fetal stages of development. Male, but not female, blastocysts collected from LPD dams displayed a significant reduction (30%) in H19 mRNA level. A significant reduction in H19 (9.4%) and Igf2 (10.9%) mRNA was also observed in male, but not in female, fetal liver at day 20 postcoitum in response to maternal LPD restricted to the preimplantation period. No effect on the blastocyst expression of Igf2R was observed in relation to maternal diet. The reduction in H19 mRNA expression did not correlate with an observed alteration in DNA methylation at the H19 differentially methylated region in fetal liver. In contrast, maternal LPD throughout 20 days of gestation did not affect male or female H19 and Igf2 imprinted gene expression in fetal liver. Neither LPD nor switched diet treatments affected H19 and Igf2 imprinted gene expression in day 20 placenta. Our findings demonstrate that one contributor to the alteration in postnatal growth induced by periconceptional maternal LPD may derive from a gender-specific programming of imprinted gene expression originating within the preimplantation embryo itself.

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“…[158][159][160] Interestingly, similar alterations in the expression of the imprinted genes H19 and IGF2 has also been reported to occur in fetal sheep due to maternal LPD during the preimplantation period. 161 Given that maternal undernutrition can lead to alteration in steroid hormone levels, 162,163 this may in turn affect H19 and/or IGF2 gene expression. Fowden and Forhead 164 highlighted the potential role of hormones as epigenetic signals in determining the phenotypical outcome of environmental cues acting during intrauterine development, as hormones signal the type, severity and duration of the environmental cue to the developing feto-placental tissues.…”

Section: Underlying Mechanisms: the Role Of Epigeneticsmentioning

confidence: 99%

The role of early life nutrition in programming of reproductive function

Chadio

Kotsampasi²

2013

J Dev Orig Health Dis

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Accumulating evidence suggest that the concept of programming can also be applied to reproductive development and function, representing an ever expanding research area. Recently issues such as peri- or even preconceptional nutrition, transgenerational effects and underlying mechanisms have received considerable attention. The present chapter presents the existed evidence and reviews the available data from numerous animal and human studies on the effects of early life nutritional environment on adult reproductive function. Specific outcomes depend on the severity, duration and stage of development when nutritional perturbations are imposed, while sex-specific effects are also manifested. Apart from undernutrition, effects of relative overnutrition as well as the complex interactions between pre- and postnatal nutrition is of high importance, especially in the context of our days obesity epidemic. Mechanisms underlying reproductive programming are yet unclear, but may include a role for epigenetic modifications. Epigenetic modulation of critical genes involved in the control of reproductive function and potential intergenerational effects represent an exciting area of interdisciplinary research toward the development of new nutritional approaches during pre- and postnatal periods to ensure reproductive health in later life.

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Ontogeny of the H19 Gene in Sheep and Effect of Maternal Fasting on Its Expression in the Fetus

Cited by 8 publications

References 37 publications

Conservation of IGF2-H19 and IGF2R imprinting in sheep: effects of somatic cell nuclear transfer

Conservation of IGF2-H19 and IGF2R imprinting in sheep: effects of somatic cell nuclear transfer

Imprinted gene expression in the rat embryo–fetal axis is altered in response to periconceptional maternal low protein diet

The role of early life nutrition in programming of reproductive function

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