Ontogeny of the nerve growth factor receptor in primary cultures of neural crest cells

Greiner, Cynthia A.M.; Lloyd, Aaron; Guroff, Gordon

doi:10.1016/0165-3806(86)90016-7

Cited by 17 publications

(1 citation statement)

References 18 publications

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“…Thus, the most straightforward explanation for the lack of a response to NGF by CA+ cells is that they lack NGF receptors. It should be noted, however, that other cells in these neural crest cultures do possess NGF receptors (End et al, 1983;Bemd, 1985Bemd, , 1986bGreiner et al, 1985. Recent data by Bemd (1986a) suggest that when neural crest cultures are grown in the presence of NGF, some TH+ cells with NGF receptors are present.…”

Section: Ca Uptake and Storage Propertiesmentioning

confidence: 95%

Phenotypic properties of catecholamine-positive cells that differentiate in avian neural crest cultures

1987

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We have investigated several phenotypic features of the catecholamine-positive (CA+) cell population that develops in quail neural crest cultures. The number, spatial distribution, and morphology of CA+ and tyrosine hydroxylase-positive (TH+) cells are similar at all ages examined, suggesting that these 2 cell classes are identical. Neither CA+ nor TH+ cell bodies or processes were stained using antisera that recognize the 70 or 160 kDa subunits of chicken neurofilament protein. Other cell bodies and fibers in the cultures (which were CA- and TH-) were stained with these neurofilament antisera. The uptake and storage of 3H-norepinephrine by neural crest cultures containing CA+ cells were inhibited in the presence of desmethylimipramine and by incubation at 0 degrees C, but were unaffected by normetanephrine. Overnight treatment with reserpine eliminated histochemically detectable CA fluorescence from the cultures. Chronic reserpine treatment from day 2 to 7 in vitro prevented the appearance of CA+ cells, while normal numbers of TH+ and somatostatin-like immunoreactive (SLI) cells developed. The number and light-microscopic morphology of the CA+ cells that developed in these cultures were not dramatically altered by either exogenous NGF or 6-hydroxydopamine. Using the method of Grillo et al. (1974), we have demonstrated that the CA+ cells observed in the light microscope corresponded to cells containing abundant cytoplasmic granular vesicles (GV) characteristic of catecholamine storage granules observed in other systems. The GV diameters were quite similar in cells examined after 5, 7, 14, and 21 d in vitro. Most GV were 50-200 nm in diameter and were distributed in a unimodal manner, with the observed modal values in the range of 85-115 nm at the ages examined. The number of GV/micron2 of cytoplasmic area remained quite constant at all ages examined. These data, taken together with other available information, suggest that the CA+ cells that differentiate in our neural crest cultures resemble, in many respects, the small, intensely fluorescent cells found in autonomic ganglia and extra-adrenal chromaffin tissue of many species. At present, we do not know if the CA+ cells that differentiate in our neural crest cultures are a stable endpoint of development or whether they are a developmental intermediate in adrenergic differentiation that is normally observed only transiently during the development of avian sympathetic ganglia in vivo, but that can persist under our tissue culture conditions.

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Section: Ca Uptake and Storage Propertiesmentioning

confidence: 95%

Phenotypic properties of catecholamine-positive cells that differentiate in avian neural crest cultures

1987

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Expression of nerve growth factor receptor in human peripheral neuropathies

Sobue

Yasuda

Mitsuma

et al. 1988

Annals of Neurology

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Nerve growth factor (NGF) receptors in human sural nerve biopsies were detected immunohistochemically using a monoclonal anti-human NGF receptor antibody. NGF receptors were not visualized within the endoneurium of normal adult nerves but were readily demonstrable within the endoneurium of nerves undergoing active axonal degeneration. Immunostaining of adjacent sections for S-100 beta protein, a specific Schwann cell marker, suggested that Schwann cells expressed the NGF receptors induced by axonal degeneration. Residual Schwann cells in nerves in which axons were completely depleted also expressed NGF receptors. NGF receptors were not detected in relation to thinly myelinated regenerating axons, nor were they detected in the endoneurium of nerves that had undergone segmental demyelination and remyelination. The increased expression of NGF receptors in human axonal neuropathies may contribute to regeneration by NGF-responsive neurons in these disorders.

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Cultured rat Schwann cells express low affinity receptors for nerve growth factor

et al. 1987

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Ontogeny of the nerve growth factor receptor in primary cultures of neural crest cells

Cited by 17 publications

References 18 publications

Phenotypic properties of catecholamine-positive cells that differentiate in avian neural crest cultures

Phenotypic properties of catecholamine-positive cells that differentiate in avian neural crest cultures

Expression of nerve growth factor receptor in human peripheral neuropathies

Cultured rat Schwann cells express low affinity receptors for nerve growth factor

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