Ontogeny of various solute carrier family (SLC) transporter expression in human pediatric liver biopsies

Abstract

This study investigates developmental changes in mRNA expression of seven SLC transporters including SLCO1B1, SLCO1B3, SLCO2B1, SLCO4A1, SLC22A7, SLC22A1, and SLC10A1 in human pediatric liver. It is hypothesized that expression of these genes undergoes developmental changes during the early years of life. Sixty‐one human pediatric liver samples were collected from living donors aged 0.3 to 12 years through liver biopsy. Real‐time RT‐PCR was used to quantify mRNA expression relative to cyclophilin for all target transporters. One‐way ANOVA with post hoc pairwise comparisons was performed to assess statistical differences in expression amongst different age groups (A: 0.3–0.7, B: 0.7–2, C: 2–5, D: 5–12 yrs). Mean normalized relative mRNA expression is presented in the table (bold figures indicate statistical significance (p<0.05)). It is concluded that in human pediatric liver, there are significant age‐dependent differences in mRNA expression of SLCO1B3 (OATP‐8) and SLCO4A1 (OATP‐E). Clinical Pharmacology & Therapeutics (2005) 77, P95–P95; doi: A(n = 9) B (n = 19) C (n = 12) D (n = 21) SLCO1B11928720353703771SLCO1B320.774.168.858.8SLCO2B10.160.400.450.52SLCO4A10.0030.0090.0140.015SLC22A71.654.234.173.31SLC22A16.1710.912.016.0SLC10A128.035.720.925.4