Ontology analysis of global gene expression differences of human bone marrow stromal cells cultured on 3D scaffolds or 2D films

Baker, Bryan A.; Pine, P. Scott; Chatterjee, Kaushik; Kumar, Girish; Lin, Nancy J.; McDaniel, Jennifer; Salit, Marc; Simon, Carl G.

doi:10.1016/j.biomaterials.2014.04.075

Cited by 32 publications

(21 citation statements)

References 46 publications

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“…13,15 Microarray testing of BMSCs demonstrated that nanofibers induced a pattern of gene expression that was similar to induction of osteogenic differentiation with biochemical supplements, and that both nanofibers and supplements lead to enrichment of genes in the Transforming Growth Factor-b pathway. 13,14,16 There is a strong link between cell shape and cell function, [17][18][19][20] and the dimensional structure of the cell niche may control cell function by influencing threedimensional (3D) shape. [21][22][23] Micropatterned cell adhesive Additional Supporting Information may be found in the online version of this article.…”

Section: Introductionmentioning

confidence: 99%

Nanofiber scaffolds influence organelle structure and function in bone marrow stromal cells

Tutak

Giri

Bajcsy

et al. 2016

J. Biomed. Mater. Res.

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Tutak, Wojtek; Jyotsnendu, Giri; Bajcsy, Peter; and Simon, Carl G. Jr., "Nanofiber scaffolds influence organelle structure and function in bone marrow stromal cells" (2016 Abstract: Recent work demonstrates that osteoprogenitor cell culture on nanofiber scaffolds can promote differentiation. This response may be driven by changes in cell morphology caused by the three-dimensional (3D) structure of nanofibers. We hypothesized that nanofiber effects on cell behavior may be mediated by changes in organelle structure and function. To test this hypothesis, human bone marrow stromal cells (hBMSCs) were cultured on poly(e-caprolactone) (PCL) nanofibers scaffolds and on PCL flat spuncoat films. After 1 dayculture, hBMSCs were stained for actin, nucleus, mitochondria, and peroxisomes, and then imaged using 3D confocal microscopy. Imaging revealed that the hBMSC cell body (actin) and peroxisomal volume were reduced during culture on nanofibers. In addition, the nucleus and peroxisomes occupied a larger fraction of cell volume during culture on nanofibers than on films, suggesting enhancement of the nuclear and peroxisomal functional capacity. Organelles adopted morphologies with greater 3D-character on nanofibers, where the Z-Depth (a measure of cell thickness) was increased. Comparisons of organelle positions indicated that the nucleus, mitochondria, and peroxisomes were closer to the cell center (actin) for nanofibers, suggesting that nanofiber culture induced active organelle positioning. The smaller cell volume and more centralized organelle positioning would reduce the energy cost of inter-organelle vesicular transport during culture on nanofibers. Finally, hBMSC bioassay measurements (DNA, peroxidase, bioreductive potential, lactate, and adenosine triphosphate (ATP)) indicated that peroxidase activity may be enhanced during nanofiber culture. These results demonstrate that culture of hBMSCs on nanofibers caused changes in organelle structure and positioning, which may affect organelle functional capacity and transport.

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Section: Introductionmentioning

confidence: 99%

Nanofiber scaffolds influence organelle structure and function in bone marrow stromal cells

Tutak

Giri

Bajcsy

et al. 2016

J. Biomed. Mater. Res.

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“…hBMSCs culture in nanofibers induced osteogenic differentiation without using osteogenic supplements, suggesting that the scaffold structure can be designed to guide cells into morphologies that direct their differentiation toward a chosen lineage . Moreover, a recent report has described that biochemical (growth factors supplementation) and physical (nanofiber) cues regulated similar ontological pathways, suggesting an overlap in the molecular mechanisms that these stimuli use to control stem cell function . It has also been demonstrated that the structural properties of the extracellular or cell neighboring microenvironment regulate cell processes and signaling pathways …”

Section: Discussionmentioning

confidence: 99%

“…Moreover, a recent report has described that biochemical (growth factors supplementation) and physical (nanofiber) cues regulated similar ontological pathways, suggesting an overlap in the molecular mechanisms that these stimuli use to control stem cell function . It has also been demonstrated that the structural properties of the extracellular or cell neighboring microenvironment regulate cell processes and signaling pathways …”

Section: Discussionmentioning

confidence: 99%

Electrospun Nanofibrous Meshes Cultured With Wharton's Jelly Stem Cell: An Alternative for Cartilage Regeneration, Without the Need of Growth Factors

Silva

Martins

Costa-Pinto

et al. 2017

Biotechnology Journal

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Many efforts are being directed worldwide to the treatment of OA-focal lesions. The majority of those efforts comprise either the refinement of surgical techniques or combinations of biomaterials with various autologous cells. Herein, we tested electrospun polycaprolactone (PCL) nanofibrous meshes for cartilage tissue engineering. For that, articular chondrocytes (hACs) isolated from human osteoarthritic joints and Wharton's Jelly Stem Cells (hWJSCs) are cultured on electrospun nanofiber meshes, without adding external growth factors. We observed higher glycosaminoglycans production and higher over-expression of cartilage-related genes from hWJSCs cultured with basal medium, when compared to hACs isolated from osteoarthritic joints. Moreover, the presence of sulfated proteoglycans and collagen type II is observed on both types of cell cultures. We believe that this effect is due to either the electrospun nanofibers topography or the intrinsic chondrogenic differentiation potential of hWJSCs. Therefore, we propose the electrospun nanofibrous scaffolds in combination with hWJSCs as a viable alternative to the commercial membranes used in autologous chondrogenic regeneration approaches.

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“…Specific culture conditions, for example low oxygen atmosphere, feeder layer, or growth factor cocktails in the medium can select for MSC entities such as multipotent adult progenitor cells, BM‐isolated adult multilineage inducible cells, or very small embryonic‐like stem cells . Moreover, it is certainly possible that culture in bioreactors or 3D culture systems may affect MSC biology compared to “standard” culture technology, that is, 2D tissue culture–treated plastic flasks …”

Section: Variable Ex Vivo Isolation and Culture Technologiesmentioning

confidence: 99%

“…15 Moreover, it is certainly possible that culture in bioreactors or 3D culture systems may affect MSC biology compared to "standard" culture technology, that is, 2D tissue culture-treated plastic flasks. 16…”

Section: Variable Ex Vivo Isolation and Culture Technologiesmentioning

confidence: 99%

Characterization of mesenchymal stem or stromal cells: tissue sources, heterogeneity, and function

Schäfer

2016

Transfusion

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Ontology analysis of global gene expression differences of human bone marrow stromal cells cultured on 3D scaffolds or 2D films

Cited by 32 publications

References 46 publications

Nanofiber scaffolds influence organelle structure and function in bone marrow stromal cells

Nanofiber scaffolds influence organelle structure and function in bone marrow stromal cells

Electrospun Nanofibrous Meshes Cultured With Wharton's Jelly Stem Cell: An Alternative for Cartilage Regeneration, Without the Need of Growth Factors

Characterization of mesenchymal stem or stromal cells: tissue sources, heterogeneity, and function

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