Oocyte‐activating capacity of fresh and frozen–thawed spermatids in the common marmoset (<i>Callithrix jacchus</i>)

Ogonuki, Narumi; Inoue, Hiroki; Matoba, Shogo; Kurotaki, Yoko; Kassai, Hidetoshi; Abe, Yukiko; Sasaki, Erika; Aiba, Atsu; Ogura, Atsuo

doi:10.1002/mrd.22971

Cited by 5 publications

(5 citation statements)

References 42 publications

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“…We have previously reported that marmoset spermatids at the late round spermatid stage or later have oocyte-activating capacity (Ogonuki et al, 2018). As expected, after injection of oocytes with late round spermatids (round spermatid injection respectively, of the oocytes were activated to form two pronuclei without any exogenous activation stimuli (Table 1).…”

Section: Introductionsupporting

confidence: 74%

“…In this study, we obtained a normal infant following ELSI, but not ICSI, probably only because ELSI embryos developed to the morula stage in vitro before embryo transfer. However, in our previous study using a 12‐month‐old male, ICSI embryos developed into blastocysts (Ogonuki et al, ). Therefore, it is likely that ICSI may also be a practical option if young donor males possess testicular spermatozoa.…”

Section: In Vitro Development Of Embryos Following Injection With Roumentioning

confidence: 92%

“…These were injected into metaphase II oocytes that had been matured for at least 18 hr in porcine oocyte medium (Research Institute for the Functional Peptides, Yamagata, Japan) supplemented with 5% fetal bovine serum and 100 IU/ml follicle‐stimulating hormone. We have previously reported that marmoset spermatids at the late round spermatid stage or later have oocyte‐activating capacity (Ogonuki et al, ). As expected, after injection of oocytes with late round spermatids (round spermatid injection [ROSI]), elongated spermatids (elongated spermatid injection [ELSI]; Figure a) or testicular spermatozoa (intracytoplasmic sperm injection [ICSI]), 63% (5/8), 69% (11/16), and 100% (3/3), respectively, of the oocytes were activated to form two pronuclei without any exogenous activation stimuli (Table ).…”

Section: In Vitro Development Of Embryos Following Injection With Roumentioning

confidence: 99%

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Birth of a marmoset following injection of elongated spermatid from a prepubertal male

Ogonuki

Abe

Kurotaki

et al. 2019

Molecular Reproduction Devel

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The common marmoset is a small nonhuman primate in which the application of transgenesis and genetic knockout techniques allows the generation of gene‐modified models of human diseases. However, its longer generation time than that of rodents is a major obstacle to the widespread use of gene‐modified marmosets for biomedical research. In this study, we examined the feasibility of shortening the generation time by using prepubertal marmoset males as gamete donors. We collected late round stage spermatids (Steps 5–7), elongated spermatids, and testicular spermatozoa from the testis of a prepubertal 11‐month‐old male marmoset and injected them into in vitro‐matured oocytes. After 7 days in culture, two embryos from elongated spermatid injection and two embryos from sperm injection were transferred into two separate recipient females. The recipient female that received elongated spermatid injection‐derived embryos became pregnant and gave birth to one female infant. This is the first demonstration that a spermatid from a prepubertal male primate can support full‐term development. Using this method, we can expect to obtain offspring of gene‐modified males 6 months to a year earlier than with natural mating

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Section: Introductionsupporting

confidence: 74%

Section: In Vitro Development Of Embryos Following Injection With Roumentioning

confidence: 92%

Section: In Vitro Development Of Embryos Following Injection With Roumentioning

confidence: 99%

See 1 more Smart Citation

Birth of a marmoset following injection of elongated spermatid from a prepubertal male

Ogonuki

Abe

Kurotaki

et al. 2019

Molecular Reproduction Devel

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“…From the perspective of reproductive physiology, non-human primates are closely related to human models; however, due to the high cost of the supply and maintenance of marmosets, a limited number of researchers are using this NHP model. Although ART studies using the marmoset model are reported, studies have been focused on the IVM of oocytes using a human IVF protocol, mainly consisting of inducing maturation using gonadotropins [9,35,36,37,38]. Nevertheless, using NHP as the model for a wider range of female reproductive diseases has arisen [39].…”

Section: Discussionmentioning

confidence: 99%

Expression of Transcripts in Marmoset Oocytes Retrieved during Follicle Isolation Without Gonadotropin Induction

Kim

Kang

Yun

et al. 2019

IJMS

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The in vitro maturation of oocytes is frequently used as an assisted reproductive technique (ART), and has been successfully established in humans and rodents. To overcome the limitations of ART, novel procedures for the in vitro maturation of early follicles are emerging. During the follicle isolation procedure, the unintended rupture of each follicle leads to a release of extra oocytes. Such oocytes, which are obtained during follicle isolation from marmosets, can be used for early maturation studies. Marmoset (Callithrix jacchus), which is classified as a new-world monkey, is a novel model that has been employed in reproductive biomedical research, as its reproductive physiology is similar to that of humans in several aspects. The ovaries of female marmosets were collected, and the excess oocytes present during follicle isolation were retrieved without pre-gonadotropin induction. Each oocyte was matured in vitro for 48 h in the presence of various concentrations of human chorionic gonadotropin (hCG) and epidermal growth factor (EGF), and the maturity of oocytes and optimal maturation conditions were evaluated. Each oocyte was individually reverse-transcribed, and the expression of mRNAs and microRNAs (miRs) were analyzed. Concentrations of hCG significantly affected the maturation rate of oocytes [the number of metaphase II (MII) oocytes]. The expression of BMP15 and ZP1 was highest when the oocytes were matured using 100 IU/L of hCG without pre-treatment with gonadotropins, and that of Cja-mir-27a was highest when cultured with follicle stimulating hormone. These results suggest that these up-regulated miRs affect the maturation of oocytes. Interactions with other protein networks were analyzed, and a strong association of BMP15 and ZP1 with sperm binding receptor (ACR), anti-Müllerian hormone (AMH), and AMH receptor was demonstrated, which is related to the proliferation of granulosa cells. Collectively, on the basis of these results, the authors propose optimal maturation conditions of excess oocytes of marmoset without in vivo gonadotropin treatment, and demonstrated the roles of miRs in early oocyte maturation at the single-cell level in marmosets.

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“…Thus, cryopreservation of elongated spermatids can potentially enable the preservation of genetic material of feline species. In addition, in the common marmoset, elongated spermatids could be collected from immature male tests [24]. In cats, it is also possible to collect elongated spermatids from immature individuals.…”

Section: Discussionmentioning

confidence: 99%

Development of feline embryos produced by Piezo-actuated intracytoplasmic sperm injection of elongated spermatids

Tsujimoto

Fujiki

Alam

et al. 2019

Journal of Reproduction and Development

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Piezo-actuated intracytoplasmic sperm injection (Piezo-ICSI) is used as an efficient in vitro fertilization method with various animals. With this method, elongated spermatids are collected from testicular tissues and are easier to obtain from animals that unexpectedly die than ejaculate sperm. Additionally, elongated spermatid injection often results in the development of embryos and offspring. To develop assisted reproductive techniques (ARTs) for domestic cats, we examined the effects of oocyte activation on cleavage and embryo development after Piezo-ICSI with motile sperm (experiment 1) and after Piezo-ICSI with either testicular sperm or elongated spermatids (experiment 2). In experiment 1, the proportions of cleaved embryos, morulas, and blastocysts following Piezo-ICSI with ethanol activation were significantly higher (P < 0.05) than in the non-activated groups. However, the proportion of blastocysts and the blastocyst quality did not differ significantly (P > 0.05) between the ethanol-activated and non-activated groups. In experiment 2, the cleavage frequencies of oocytes after Piezo-ICSI of testicular sperm or elongated spermatids and ethanol activation were higher (P < 0.05) than that of oocytes in the non-activated group, but the occurrence of blastocyst formation and quality of blastocysts did not differ between the activated and non-activated groups. In summary, cat embryos can be produced by Piezo-actuated microinjection of elongated spermatids. Ethanol activation increased the frequency of cleavage, but it affected neither the occurrence of blastocyst development nor the quality of blastocysts. These results represent an expansion in the repertoire of ARTs that are potentially applicable to both domestic and endangered species of cats.

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Oocyte‐activating capacity of fresh and frozen–thawed spermatids in the common marmoset (Callithrix jacchus)

Cited by 5 publications

References 42 publications

Birth of a marmoset following injection of elongated spermatid from a prepubertal male

Birth of a marmoset following injection of elongated spermatid from a prepubertal male

Expression of Transcripts in Marmoset Oocytes Retrieved during Follicle Isolation Without Gonadotropin Induction

Development of feline embryos produced by Piezo-actuated intracytoplasmic sperm injection of elongated spermatids

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