Oocyte and Embryo Quality: Effect of Origin, Culture Conditions and Gene Expression Patterns

Lonergan, Patrick; Rizos, Dimitrios; Gutiérrez‐Adán, Alfonso; Fair, Trudee; Boland, M.P.

doi:10.1046/j.1439-0531.2003.00437.x

Cited by 271 publications

(198 citation statements)

References 115 publications

(211 reference statements)

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“…The ability of in vitro produced embryos to survive cryopreservation, an indicator of embryo quality [4,5,8,12,41,42], is affected by culture conditions. Thus, Dinnyés et al [29] reported high survival rates (85% and 90%) after vitrification/warming of Day 8 expanded embryos produced in SOF without and with FCS, respectively; the hatching rates the above authors obtained for embryos produced in SOF + FCS (63%) are comparable to the survival rates in our work in the Vero and BSA groups.…”

Section: Discussionmentioning

confidence: 99%

Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification

et al. 2008

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The aim of this study was to examine the effects of co-culture with Vero cells during the in vitro maturation (IVM) and three culture media, B2 + 5% fetal calf serum (FCS) on Vero cells, synthetic oviduct fluid (SOF) + 5% FCS, and SOF + 20 g L À1 bovine serum albumin (BSA), on the developmental competence of the embryos and their ability to survive vitrification/warming. We also tested the effect of morphological quality and the age of the embryo on its sensitivity to vitrification. The IVM system neither affects the embryo development up to Day 7 nor survival rates after vitrification. The culture of embryos in SOF + FCS and in Vero cells + B2 allowed obtaining more Day 6 and Day 7 blastocysts, and a higher % of Day 7 blastocysts vitrified than culture in SOF + BSA. Contrarily, on Day 8, more blastocysts were vitrified in SOF + BSA than in SOF + FCS. Blastocysts quality affected development after vitrification/warming, and Day 7 embryos showed higher survival rates than their Day 8 counterparts. Day 7 blastocysts produced in Vero cells or in SOF + BSA survived at higher rates than those produced in SOF + FCS at 24 and 48 h after warming. Embryo culture with BSA allows obtaining hatching rates after vitrification/warming higher than those obtained after coculture with Vero cells in B2 and FCS. Moreover, this system provides hatching rates from Day 8 blastocysts comparable to those obtained on Day 7 in Vero cells. Further studies, including embryo transfer to recipients, are needed to clarify factors affecting the freezability of in vitro produced bovine embryos. #

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Section: Discussionmentioning

confidence: 99%

Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification

et al. 2008

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“…These events start with the ability of the oocyte to complete maturation and then to undergo successful fertilization, cleavage and reach the blastocyst stage producing a good-quality embryo (Sirard et al, 2006). Although succeeding in one of these events does not guarantee success in the next, oocyte maturation is considered a critical step that influences the success of fertilization and subsequent embryo development (Lonergan et al, 2003). While $60% of in-vivo matured bovine oocytes reach the blastocyst stage when fertilized and cultured in vitro, less than 40% of oocytes matured in vitro do so.…”

Section: Introductionmentioning

confidence: 99%

Role of PTGS2-generated PGE2 during gonadotrophin-induced bovine oocyte maturation and cumulus cell expansion

Marei

Abayasekara

Wathes

et al. 2014

Reproductive BioMedicine Online

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(AA Fouladi-Nashta).Waleed Fawzy A Marei is a veterinarian and graduated from Cairo University, Egypt in 2001. He started his academic career in theriogenology in 2002 and obtained his MSc degree in 2005. He joined the reproduction research group at the Royal Veterinary College, London, UK and investigated the effects of fatty acids on oocyte quality and early embryo development for his PhD, awarded in 2010. He then pursued post-doctoral research in the same group, working on early embryo development and implantation. Currently, he is a lecturer on theriogenology at Cairo University. His interests are clinical applications of assisted reproduction technologies and feed additives to improve reproductive performance in farm animals.Abstract Prostaglandin E 2 (PGE 2 ) is an autocrine/paracrine factor which mediates gonadotrophin (Gn) stimulation of cumulus expansion and oocyte maturation in rodents. Its role in bovine oocyte maturation is less characterized. This study detected PTGS2 (COX2) and PGE synthases (PTGES1, PTGES2 and PTGES3) in bovine cumulus-oocyte complexes (COC). Only PTGS2 and PTGES1 expression changed during maturation. In Gn-free media, no cumulus expansion and $45% nuclear maturation was achieved, while Gn-induced maturation showed full cumulus expansion (score 3) and $87% maturation. PGE 2 supplementation without Gn induced mild cumulus expansion (score 0.5-1) but increased nuclear maturation to levels similar to those obtained with Gn alone. In the presence of Gn, exogenous PGE 2 did not affect expansion or nuclear maturation and subsequent embryo development. Treatment with PTGS2 selective inhibitor (NS398), PTGS2-specific siRNA or PTGER2-receptor antagonist (AH6809) resulted in $20-25% reduction in nuclear maturation. NS398 and AH6809 did not affect cumulus expansion. Most oocytes not reaching metaphase of second meiosis (MII) following NS398, AH6809 and PTGS2-specific siRNA treatments were at MI. After longer maturation, NS398-treated oocytes had normal MII rate and uncompromised embryo development. PGE 2 has a limited role in cumulus expansion in bovine COC but is important for the timing of Gn-induced nuclear maturation. RBMOnline

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“…The ability to achieve such development, known as oocyte competence, is acquired mainly during the antral phase of folliculogenesis through wellcoordinated molecular processes, including proper nuclear maturation and final cytoplasmic maturation (Sirard et al 2003, Krisher 2004, Kimura et al 2007. Successful oocyte final maturation is therefore a complex process that implies many factors such as intrinsic oocyte quality (Lonergan et al 2003, Wang & Sun 2007, dialog with neighboring somatic compartment (Matzuk et al 2002, Tanghe et al 2002, Yokoo & Sato 2004, Gilchrist et al 2008, Li et al 2008, and functional gap junctions (Thomas et al 2004, Lodde et al 2007. It has been established that, inside the follicle, the oocyte is surrounded by granulosa cell (GC) populations that, through folliculogenesis, differentiate into both mural GCs and cumulus cells (CCs).…”

Section: Introductionmentioning

confidence: 99%

Cumulus cell gene expression following the LH surge in bovine preovulatory follicles: potential early markers of oocyte competence

Assidi¹,

Dieleman²,

Sirard³

2010

Reproduction

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Cumulus cells (CCs) are essential for oocytes to reach full development competency and become fertilized. Many major functional properties of CCs are triggered by gonadotropins and governed by the oocyte. Consequently, cumulus may reflect oocyte quality and is often used for oocyte selection. The most visible function of CCs is their ability for rapid extracellular matrix expansion after the LH surge. Although unexplained, LH induces the final maturation and improves oocyte quality. To study the LH signaling and gene expression cascade patterns close to the germinal vesicle breakdown, bovine CCs collected at 2 h before and 6 h after the LH surge were hybridized to a custom-made microarray to better understand the LH genomic action and find differentially expressed genes associated with the LH-induced oocyte final maturation. Functional genomic analysis of the 141 overexpressed and 161 underexpressed clones was performed according to their molecular functions, gene networks, and cell compartments. Following real-time PCR validation of our gene lists, some interesting pathways associated with the LH genomic action on CCs and their possible roles in oocyte final maturation, ovulation, and fertilization are discussed. A list of early potential markers of oocyte competency in vivo and in vitro is thereafter suggested. These early biomarkers are a preamble to understand the LH molecular pathways that trigger the final oocyte competence acquisition process in bovine.

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Oocyte and Embryo Quality: Effect of Origin, Culture Conditions and Gene Expression Patterns

Cited by 271 publications

References 115 publications

Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification

Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification

Role of PTGS2-generated PGE2 during gonadotrophin-induced bovine oocyte maturation and cumulus cell expansion

Cumulus cell gene expression following the LH surge in bovine preovulatory follicles: potential early markers of oocyte competence

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