Oocyte biology and challenges in developing in vitro maturation systems in the domestic dog

Songsasen, Nucharin; Wildt, David E.

doi:10.1016/j.anireprosci.2006.10.004

Cited by 85 publications

(76 citation statements)

References 116 publications

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“…Since that report, and despite numerous studies documenting and focusing on establishing a suitable system for IVM of canine oocytes [15][16][17][18][19], the efficiency is lower than for other domestic mammalian species, for example cattle [20,21], sheep [22], pigs [23], mice [24], and cats [25]. Although puppies have been produced from somatic cell nuclear transfer of in-vivo matured oocytes [26], there has been no report of the production of live young after transfer of in-vitro maturation and fertilization-derived embryos [27].…”

Section: Introductionmentioning

confidence: 99%

Effect of bovine cumulus–oocyte complexes‐conditioned medium on in‐vitro maturation of canine oocytes

Abdel-Ghani

Abe

Asano

et al. 2010

Reprod Medicine & Biology

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Purpose To investigate the ability of medium conditioned with bovine cumulus-oocyte complexes (COCs) to support nuclear maturation of canine oocytes recovered from domestic dog ovaries. Methods Cumulus-oocyte complexes were obtained from ovaries of domestic bitches (8 months old to 7 years old), and in-vitro maturation was evaluated in TCM-199 supplemented with different concentrations (0, 20, 30 or 50%) of bovine COCs-conditioned medium (BCM). The canine COCs were cultured for 72 or 96 h at 38.5°C in 5% CO 2 , 5% O 2 and 90% N 2 . The bovine COCs-conditioned medium was obtained from culture of bovine COCs with TCM-199 supplemented with 5% FCS for 22 h at 38.5°C in 2% CO 2 , 98% air. Results The proportion of germinal vesicle breakdown (GVBD) after 72 h was significantly higher (P \ 0.05) in medium supplemented with 30% BCM (20.7%) compared with the control group (13.4%). The rates of GVBD-MII stage were significantly higher (P \ 0.05) when oocytes were matured with BCM at concentration of 30% (41.5%) compared with control (26.6%) after 72 h in-vitro culture. After 96 h in-vitro culture, the oocytes matured in medium supplemented with 30% BCM (5.5%) showed a significant increase (P \ 0.05) in the proportion of MII compared with control (0.7%). However, increasing the cultivation time from 72 to 96 h resulted in an increase in oocyte degeneration rate. Conclusions The results suggested that bovine COCsconditioned medium supplementation significantly increased nuclear maturation of canine oocytes.

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Section: Introductionmentioning

confidence: 99%

Effect of bovine cumulus–oocyte complexes‐conditioned medium on in‐vitro maturation of canine oocytes

Abdel-Ghani

Abe

Asano

et al. 2010

Reprod Medicine & Biology

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“…From 1976 (Mahi and Yanagimaci, 1976) to date, numerous studies have been conducted to improve success of in vitro maturation (IVM) of bitch oocytes but an efficient protocol for IVM has not been established. In vitro maturation, generally has resulted in low rates of successful resumption of meiosis, with the exception being one study that found that use of oocytes from follicles larger than 2 mm yielded significantly higher rates of metaphase II development in vitro (Songsasen and Wildt, 2005), where 79.5% of oocytes recovered from follicles 2 mm size reached the second metaphase (MII), usually less than 20% of canine oocytes achieve nuclear maturation (Songsasen and Wildt, 2007). Moreover, only a single blastocyst has been produced after in vitro fertilization of in vitro matured oocytes (Otoi et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

The influence of ascorbic acid on in vitro maturation of canine oocytes

Pernes¹,

Miclea²,

Zăhan³

et al. 2016

BUASVMCN-ASB

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It is known that L-ascorbic acid (vitamin C) can modulate many biochemical processes intracellularly or extracellularly as antioxidant. The aim of the present study was to examine the effects of media supplementation with ascorbic acid on canine oocyte meiotic maturation, viability and the cumulus cell expansion. Various concentrations of ascorbic acid supplemented in in vitro maturation (IVM) media were tested. Canine oocyte was exposed to different levels of ascorbic acid (0, 50, 150, 250, 500, 750µM). Cumulus expansion, meiotic maturation and degeneration of oocytes were assessed 72 h after in vitro culture. As results, on the group treated with 250µM ascorbic acid was a significant difference compared to the control group on nuclear maturation in stages metaphase I (MI) and metaphase II (MII) (26.98% vs. 6.00%). The groups treated with 50, 150, 250, 500µM had an increase in stage (GVBD), and a significant decrease of degenerate-undefined oocytes compared with the control (23.31%, 18.85%, 13.41% vs 40.80). Concentration 750µM had similar effect to that in the control group. The groups treated with 50, 150, 250, 500µM had an increase in meiosis resumption (GVBD), metaphase I (MI) and metaphase II (MII) with the best result in the group treated with 250 µM ascorbic acid.

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“…Due to the high specificity of reproductive cycles, the reported symptoms have been somewhat different for various species investigated (Grundy et al 2002, Songsasen and Wildt 2007, Zanghf et al 2007). …”

Section: Discussionmentioning

confidence: 99%

The effects of experimental administration of low doses of zearalenone on the histology of ovaries in pre-pubertal bitches

Gajęcka¹

2013

Polish Journal of Veterinary Sciences

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The experiment involved 30 clinically healthy Beagle bitches aged approximately 70 days with an initial body weight of approximately 8 kg. The animals were randomly divided into two experimental groups (EI and EII) and a control group of 10 animals each. Group EI was administered 50 μg of body weight zearalenone/kg per os for 42 days, group EII received 75 μg of body weight zearalenone/kg per os for 42 days, and the control group was administered placebo per os for 42 days. The bitches were ovariectomized at the end of the treatment period for anatomopathological examination. At the same time, peripheral blood samples were collected for endocrinological analyses (17β-estradiol and progesterone). Administartion of zearalenone particularly higher doses, resulted in the hyperestrogenism degeneration and atrophy of ovarian cells and tissues with accompanying edema and blood extravasation, leading to increased 17β-estradiol concentrations and an insignificant decrease in progesterone levels.

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Oocyte biology and challenges in developing in vitro maturation systems in the domestic dog

Cited by 85 publications

References 116 publications

Effect of bovine cumulus–oocyte complexes‐conditioned medium on in‐vitro maturation of canine oocytes

Effect of bovine cumulus–oocyte complexes‐conditioned medium on in‐vitro maturation of canine oocytes

The influence of ascorbic acid on in vitro maturation of canine oocytes

The effects of experimental administration of low doses of zearalenone on the histology of ovaries in pre-pubertal bitches

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