Oocyte development in bovine primordial follicles is promoted by activin and FSH within a two-step serum-free culture system

McLaughlin, Marie; Telfer, Evelyn E.

doi:10.1530/rep-10-0025

Cited by 118 publications

(82 citation statements)

References 45 publications

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“…It has been shown that oocyte donor breed affects its developmental competence in other species (Ptak et al, 2003;Rátky et al, 2005). However, our results were within the average rates previously reported in other cattle breeds (Camargo et al, 1997;Kafi et al, 2002;Wang et al, 2007;McLaughlin & Telfer, 2010;Nicodemo et al, 2010). It may suggest that donor breed may not produce an important influence in the in vitro oocyte nuclear maturation in some bovine populations.…”

supporting

confidence: 72%

Short communication: In vitro oocyte maturation and fertilization rates in the Spanish Lidia bovine breed

Demyda-Peyrás

Hidalgo

Dorado

et al. 2013

Span. j. agric. res.

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The Lidia bovine breed is the most successful cattle breed on the Iberian Peninsula, also considered a hallmark of Spanish tradition and image around the world. The aims of the study were to characterize the oocyte recovery rates and to evaluate the effect of two standard in vitro maturation protocols on oocyte maturation (cumulus expansion and nuclear maturation) and fertilization rates after in vitro fertilization in this breed. For this purpose, 261 ovaries from Lidia cows were processed obtaining 1,125 viable cumulus oocyte complexes (COCs). The oocyte recovery rate obtained (4.31 viable COCs per ovary) was lower than those described previously in other studied breeds. Maturation rates were evaluated in two different oocyte maturation media with (M1) and without (M2) hormonal supplementation. The percentage of COCs with expanded cumulus cells was significantly lower in M1 (74.35%) compared with M2 (82.25%). Metaphase II (MII) rates (67.75% in M1 and 73.18% in M2) were similar to previous studies in different cattle populations. M2 significantly improved the percentage of COCs with their cumulus cells expanded (p<0.01) and nuclear maturation rates (p<0.05), but it did not affect the fertilization percentages obtained in this experiment. In conclusion, our study suggests that oocytes of the Lidia cattle breed can be obtained, matured and fertilized following standard protocols previously used in other cattle breeds.

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supporting

confidence: 72%

Short communication: In vitro oocyte maturation and fertilization rates in the Spanish Lidia bovine breed

Demyda-Peyrás

Hidalgo

Dorado

et al. 2013

Span. j. agric. res.

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“…This has been demonstrated to some extent in the mouse but some somatic cells must be present for the full capacity to develop. The culture of entire follicles has been studied and progress has been made, especially in species where tissues are readily available, such as mouse and cow [18,19].…”

Section: The Links Between Follicle and Oocytementioning

confidence: 99%

Follicle environment and quality of in vitro matured oocytes

Sirard

2011

J Assist Reprod Genet

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In mammalian reproduction, the oocyte depends on the ovarian follicle for most of its growth. They form a bipolar partnership and the status of one will impact the functioning of the other. When oocytes are removed from their follicle by ovulation, they have normally completed all the steps required to begin their journey into the oviduct and drive the early embryonic development. When oocytes are removed from their follicle before natural ovulation, the process by which they acquire all the important components for their journey might not be completed and their ability to mature, fertilize or develop into embryos or to term might be compromised. Animal models have been useful to define the important steps required for the oocyte's growth phase, and in the mouse, when the oocyte has reached its full size, the program is ready. This is not the case in larger mammals where the completion of growth does not ensure that the oocyte is fully capable of undergoing all the steps to the embryo and to term. The final steps of oocyte preparation also involve a progressive condensation of the chromatin that may facilitate normal maturation but may also indirectly reduce the lifespan of the oocyte. In such a scenario, the oocyte would have an expiration date when fully competent. In humans, a number of indications may justify the aspiration of oocytes from unstimulated patients and the development of an in vitro maturation (IVM) process that would allow fertilization and subsequent development. This objective could be realized by a better understanding of the essential follicular contribution required before removing the oocyte. Therefore, this review will focus on the large animal models where IVM has been used and studied for more than 25 years. The status of the follicle at the time of oocyte recovery and the status of the oocyte's chromatin will be described in detail as they have a significant impact on the outcome.

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“…Alternatively, growth continues until follicle/oocyte-granulosa cell complexes form a dome-like structure, as previously reported in mice [14][15][16][17], rats [18], cattle [8], and pigs [19]. In the sphere system, follicles develop an antral cavity when subjected to appropriate stimulation in mice [3,[20][21][22][23][24], cattle [25][26][27][28], pigs [29, 30], sheep [31, 32], goats [33, 34], and humans [35, 36]. To achieve three-dimensional culture, follicles are often embedded in collagen- [29, 37] or alginate-based matrices [36, 38, 39].…”

mentioning

confidence: 58%

“…Activins are also known to be involved in follicular regulation [73]. Although some positive effects of activin A on preantral follicle growth have been reported [28,35,74], impaired follicle viability because of activin A treatment has also been reported [75]. Estradiol [13] and androstenedione [76,77] are other potent stimulators of follicular growth in vitro that will be discussed later.…”

Section: Granulosa Cellsmentioning

confidence: 99%

Isolation of Ovarian Components Essential for Growth and Development of Mammalian Oocytes <i>In Vitro</i>

Hirao

2012

Journal of Reproduction and Development

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Abstract. Mammalian ovaries contain a large number of oocytes, most of which degenerate either before or at various stages of growth. Dynamic and precise regulation in the ovary involves many factors, each with a unique role. Identifying the single most important factor is impossible; however, it may be possible to identify factors essential for oocyte growth. It is evident that oocytes can grow into competent ova in vitro; however, how faithfully the follicle should mimic the in vivo conditions remains unclear. In the culture system discussed in this review, bovine and mouse oocyte-granulosa cell complexes, at approximately the late mid-growth stage, spread on a substratum without the involvement of theca cells. The structural simplicity of this system is advantageous because it reduces the basic conditions essential for regulation of oocyte growth. Apart from biological factors, high concentrations of polyvinylpyrrolidone (molecular weight: 360000) improved oocyte growth. Among ovarian factors, androstenedione was used to compensate for the absence of theca cells, and it promoted both follicular growth and acquisition of oocyte meiotic competence. Most oocytes cultured in a group were viable after long-term culture, suggesting that unlike ovarian events, there was no exhaustive follicle selection. Collectively, oocytes and their associated granulosa cells can establish independent units capable of supporting oocyte growth in appropriately modified culture media. Key words: In vitro, Oocyte-granulosa cell complexes, Oocyte growth, Polyvinylpyrrolidone (J. Reprod. Dev. 58: [167][168][169][170][171][172][173][174] 2012) I n the mammalian ovary, a small proportion of primordial oocytes periodically enter the growth phase and even fewer grow, mature, and ovulate [1]. Various factors, including gonadotrophins, growth factors, and steroid hormones, underlie the dynamic regulation of oocyte and follicular growth and their selection. The regulation is so dynamic and precise that examination of these events in situ is extremely difficult.One solution to this problem is to isolate oocytes and their associated ovarian cells and culture them in vitro. It is well established that oocytes can grow in vitro because "normal" ova have been produced in vitro in mice [2][3][4][5][6] and cattle [7,8] (oocytes were cultured for the latter half of their growth period). In mice, offspring have even been derived from oocytes cultured from primordial follicles [9,10]. It is thus clear that requirements for oocyte growth can be studied in vitro. In addition, it is possible to isolate ovarian components essential for oocyte growth and acquisition of developmental competence in vitro. However, how faithfully the follicle should mimic the in vivo conditions remains unclear. This review focuses on culture systems with the smallest possible functional structure effective to support oocyte growth. Two Major Types of Culture SystemsSeveral culture systems have been developed for in vitro oocyte growth [11]. They can be broadly divided into ...

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Oocyte development in bovine primordial follicles is promoted by activin and FSH within a two-step serum-free culture system

Cited by 118 publications

References 45 publications

Short communication: In vitro oocyte maturation and fertilization rates in the Spanish Lidia bovine breed

Short communication: In vitro oocyte maturation and fertilization rates in the Spanish Lidia bovine breed

Follicle environment and quality of in vitro matured oocytes

Isolation of Ovarian Components Essential for Growth and Development of Mammalian Oocytes <i>In Vitro</i>

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