Oocyte environment: follicular fluid and cumulus cells are critical for oocyte health

Dumesic, Daniel A.; Meldrum, David R.; Katz‐Jaffe, Mandy G.; Krisher, Rebecca L.; Schoolcraft, William B.

doi:10.1016/j.fertnstert.2014.11.015

Cited by 502 publications

(362 citation statements)

References 262 publications

(282 reference statements)

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“…Data are described as median (range). a p < 0.05 compared to <35; b p < 0.05 compared to [35][36][37][38][39] …”

Section: Discussionmentioning

confidence: 99%

“…Cumulus GCs play a vital role in regulating oocyte maturation, and the health of the cumulus cell determines oocyte quality [35,36]. Although not fully defined yet, cumulus GCs express characteristics distinct from mural GCs, which are steroidogenically more active [37,38].…”

Section: Discussionmentioning

confidence: 99%

“…Although not fully defined yet, cumulus GCs express characteristics distinct from mural GCs, which are steroidogenically more active [37,38]. Cumulus cells communicate with each other and with the oocyte through specialized gap junctions that allow metabolic exchange and transport of signaling molecules for appropriate follicular development [35,36,39]. Interestingly, cumulus and mural GCs have been shown to reflect the characteristics of the oocyte thus providing a noninvasive means to assess oocyte quality [35].…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Ovarian kisspeptin expression is related to age and to monocyte chemoattractant protein-1

Merhi

Thornton

Bonney

et al. 2016

J Assist Reprod Genet

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Purpose The objective of this study was to test the hypothesis that ovarian kisspeptin (kiss1) and its receptor (kiss1r) expression are affected by age, obesity, and the age-and obesityrelated chemokine monocyte chemoattractant protein-1 (MCP-1). Methods Ovaries from reproductive-aged and older C57BL/ 6J mice fed normal chow (NC) or high-fat (HF) diet, ovaries from age-matched young MCP-1 knockout and young control mice on NC, and finally, cumulus and mural granulosa cells (GCs) from women who underwent in vitro fertilization (IVF) were collected. Kiss1, kiss1r, anti-Mullerian hormone (AMH), and AMH receptor (AMHR-II) messenger RNA (mRNA) expression levels were quantified using real-time polymerase chain reaction (RT-PCR).Results In mouse ovaries, kiss1 and kiss1r mRNA levels were significantly higher in old compared to reproductive-aged mice, and diet-induced obesity did not alter kiss1 or kiss1r mRNA levels. Compared to young control mice, young MCP-1 knockout mice had significantly lower ovarian kiss1 mRNA but significantly higher AMH and AMHR-II mRNA levels. In human cumulus GCs, kiss1r mRNA levels were positively correlated with age but not with BMI. There was no expression of kiss1 mRNA in either cumulus or mural GCs. Conclusion These data suggest a possible age-related physiologic role for the kisspeptinergic system in ovarian physiology. Additionally, the inflammatory MCP-1 may be associated with kiss1 and AMH genes, which are important in ovulation and folliculogenesis, respectively.

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“…Data are described as median (range). a p < 0.05 compared to <35; b p < 0.05 compared to [35][36][37][38][39] …”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Ovarian kisspeptin expression is related to age and to monocyte chemoattractant protein-1

Merhi

Thornton

Bonney

et al. 2016

J Assist Reprod Genet

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“…Some studies showed that the intrinsic ovarian problems is accompanied with reduced endometrial receptivity, and this is the cause of low rate of pregnancy in endometriosis patients [48,49]. Given the fact that the physiological role of aromatase and subsequently estrogen production by granulosa and cumulus cells in follicular microenvironment are fundamental for the oocyte cytoplasmic maturation, fertilization process, and early-stage embryo development [50][51][52], it is not improbable to speculate that altered expression and disturbed such functions of aromatase might contribute to follicular growth deficiency and decreased oocytes quality. In this context, Barcelos et al demonstrated that the reduced expression of aromatase in CCs of endometriosis patient might be responsible for the oocyte deficiency, lower number of fertilized oocytes, and cleaved embryos and, subsequently, the infertility in such patients [26].…”

Section: Discussionmentioning

confidence: 99%

Epigenetic alterations of CYP19A1 gene in Cumulus cells and its relevance to infertility in endometriosis

Hosseini

Mehraein

Shahhoseini

et al. 2016

J Assist Reprod Genet

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Purpose The purpose of the present study was to investigate the epigenetic mechanisms responsible for the aberrant aromatase expression (CYP19A1) in Cumulus Cells (CCs) of infertile endometriosis patients. Method Cumulus cells were obtained from 24 infertile patients with and without endometriosis who underwent ovarian stimulation for intracytoplasmic sperm injection. Expression of CYP19A1 gene was quantified using Reverse Transcription Q-PCR. DNA methylation, histone modifications, and binding of Estrogen Receptor, ERβ to regulatory DNA sequences of CYP19A1 gene were evaluated by Chromatin ImmunoPrecipitation (ChIP) assay.Results CYP19A1 gene expression in CCs of endometriosis patients was significantly lower than the control group (P = 0.04). Higher incorporation of MeCP2 (as a marker of DNA methylation) on PII and PI.4 promoters, and hypoacetylation at H3K9 in PII and hypermethylation at H3K9 in PI.4 were observed in CYP19A1 gene in endometriosis patients (P < 0.05). Moreover, a decreased level of ERβ binding to PII and an increased level of its binding to PI.3 and PI.4 promoters of CYP19A1 were observed in endometriosis patients when compared to control. Conclusion Significant reduction of CYP19A1 gene expression in CCs of endometriosis patients may be the result of epigenetic alterations in its regulatory regions, either by DNA methylation or histone modifications. These epigenetic changes along with differential binding of ERβ (as a transcription factor) in CYP19A1 promoters may impair follicular steroidogenesis, leading to poor Oocyte and embryo condition in endometriosis patients.

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“…Estudos em humanos tem mostrado a relação entre presença de citocinas, fatores de crescimento, proteínas e metabolitos com a qualidade do ovócito (Benkhalifa et al, 2015;Dumesic et al, 2015). Em bovinos, também tem sido relatado que a análise metobolômica do LF pode ser uma ferramenta útil para caracterizar a qualidade do ovócito (Sinclair et al, 2014;Bender et al, 2010).…”

Section: Marcadores Bioquímicos No Líquido Folicularunclassified

Marcadores moleculares para qualidade de ovocitos bovino

Sprícigo¹,

Dode²

2017

SPERMOVA

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A seleção de ovócitos baseada em critérios morfológicos tem sido o método utilizado até o presente, no entanto, esse método apresenta limitações quanto a acurácia, objetividade e constância. Dessa forma, existe a necessidade de se buscar outras ferramentas que permitam uma seleção mais acurada de ovócitos competentes. Considerando que o ambiente folicular pode refletir características do ovócito, esse pode fornecer alternativas para a avaliação não invasiva da qualidade do ovócito. Nesse sentido, os esforços estão concentrados no perfil transcricional de células do cumulus (CC) e células da granulosa (CGM) e no perfil bioquímico do liquido folicular (LF). Entre as abordagens estão a análise do transcriptoma, proteoma e metaboloma. Nessa revisão inicialmente são descritos estudos que visam identificar marcadores no próprio ovócito, que apesar de fornecerem informações importante para o entendimento sobre a regulação da capacidade de desenvolvimento, inviabilizam o uso subsequente do ovócito. Posteriormente, são revisados os estudos que avaliam a análise transcricional das CCs e da CMG em correlação com a competência do ovócito e, em especial, os que acompanham o desenvolvimento embrionário para validar marcadores. E, finalmente é abordada a possibilidade de se identificar marcadores bioquímicos no LF e a tendência dos últimos anos, que tem passado de estudo marcadores únicos para técnicas mais complexas que estudam todos os metabólitos de LF utilizando a metabolômica como ferramenta. Palavra-chave: competência, ovócito, célula do cumulus, líquido folicular. ABSTRACTOocyte selection using morphological criteria is currently used, but this selection approach has limitations in terms of accuracy, objectivity, and constancy. Therefore, the development of new methods that could allow the identification of molecular markers that can predict the oocyte competency more accurately is needed. Considering that follicular environment may reflects oocyte status, it can provide new alternatives for non-invasive assessment of oocyte quality. The efforts are concentrated on determining transcriptional profiling of cumulus (CC) and granulosa cells (MGC) and on biochemical profiling of follicular fluid (FF). The approaches used are transcriptomics, proteomics, and metabolomics technologies. In this review, we first describe the studies that aimed to identify molecular markers on the oocyte itself, that although gives relevant information regarding to the understanding of competency acquisition, they destroy the oocyte. Then, studies that evaluated cumulus/granulosa cell gene expression and especially those that followed the embryo development until the blastocyst stage are reported. Lastly, the possibility of identifying markers on FF is discussed as well as the tendency observer on the last few years of evaluating not only one metabolite but all metabolites using metabolomics as tool.

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Oocyte environment: follicular fluid and cumulus cells are critical for oocyte health

Cited by 502 publications

References 262 publications

Ovarian kisspeptin expression is related to age and to monocyte chemoattractant protein-1

Ovarian kisspeptin expression is related to age and to monocyte chemoattractant protein-1

Epigenetic alterations of CYP19A1 gene in Cumulus cells and its relevance to infertility in endometriosis

Marcadores moleculares para qualidade de ovocitos bovino

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