Oocyte karyotyping by comparative genomic hybrydization provides a highly reliable method for selecting “competent” embryos, markedly improving in vitro fertilization outcome: a multiphase study

Sher, Geoffrey; Keskintepe, Levent; Keskintepe, Meral; Ginsburg, Michael; Maassarani, Ghanima; Yakut, Tahsin; Baltacı, Volkan; Kotzé, Dirk; Ünsal, Evrim

doi:10.1016/j.fertnstert.2006.08.108

Cited by 91 publications

(64 citation statements)

References 31 publications

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“…However, including both sex chromosomes, there are 23 other chromosomes that could contribute to the rate of trisomy and aneuploidy during embryo development. Sher et al performed oocyte and embryo numeric karyotyping using the recently developed technique of comparative genomic hybridization (CGH) [6]. This method enables determination of the number of copies of all the chromosomes.…”

Section: Introductionmentioning

confidence: 99%

The contribution of mitochondrial function to reproductive aging

Bentov

Yavorska

Esfandiari

et al. 2011

J Assist Reprod Genet

171

125

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Purpose The number of women attempting to conceive between the ages of 36 and 44 has increased significantly in the last decade. While it is well established that women's reproductive success dramatically declines with age, the underlying physiological changes responsible for this phenomenon are not well understood. With assisted reproductive technologies, it is clear that oocyte quality is a likely cause since women over 40 undergoing in vitro fertilization (IVF) with oocytes donated by younger women have success rates comparable to young patients. Apart from oocyte donation, there is no known intervention to improve the pregnancy outcome of older patients. The aim of this paper was the review the relevant data on the potential role of mitochondria in reproductive aging. Method Review of current literature on the subject. Results We present the current evidence that associate mitochondrial dysfunction with age related decrease in female reproductive outcome. Conclusions The aging process is complex, driven by a multitude of factors thought to modulate cellular and organism life span. Although the factors responsible for diminished oocyte quality remain to be elucidated, the present review focuses on the potential role of impaired mitochondrial function.

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Section: Introductionmentioning

confidence: 99%

The contribution of mitochondrial function to reproductive aging

Bentov

Yavorska

Esfandiari

et al. 2011

J Assist Reprod Genet

171

125

View full text Add to dashboard Cite

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“…7 Assisted reproduction technologies require the reproductive quality of oocytes to be evaluated to select the healthy ones for in vitro fertilization (IVF) use. Existing techniques for oocyte quality assessment include morphology analysis, 25,27 genetic screening, 5,29 spectroscopy-based metabolomic profiling, 22,28,33 and polscope-based spindle imaging. 20,24 These techniques have the following limitations: (i) morphological analysis is often subjective and fails to provide definitive prediction for oocyte quality; (ii) genetic screening employs a deoxyribonucleic acid (DNA) sampling procedure that may impair the oocyte and result in lower development competence 18 ; and (iii) metabolomic profiling and spindle imaging demand specific analysis equipment and usually involve complex spectral data analysis.…”

Section: Introductionmentioning

confidence: 99%

Elastic and Viscoelastic Characterization of Mouse Oocytes Using Micropipette Indentation

et al. 2012

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Abstract-This paper reports the first quantitative comparison study of elastic and viscoelastic properties of oocytes from young and aged mice. A force measurement technique, including a poly(dimethylsiloxane) (PDMS) cell holding device and a sub-pixel computer vision tracking algorithm, is utilized for measuring forces applied to an oocyte and resultant cell deformations in real time during oocyte manipulation. To characterize elastic and viscoelastic properties of the oocytes, a stress-relaxation indentation test is performed. A two-step, large-deformation mechanical model is developed to extract the mechanical properties of the oocytes from the measured force-deformation data. The experimental results demonstrate that the aged oocytes are significantly softer (instantaneous modulus: 2.2 vs. 5.2 kPa in young oocytes) but more viscous (relaxation time: 4.1 vs. 2.3 s in young oocytes) than the young oocytes.

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“…It has been well established that both implantation and pregnancy rates can be improved by selectively transferring only those embryos that have developed into blastocysts stage 5-6 days post-fertilization in vitro [2][3][4][5][6][7]. We recently reported that >90% of embryos that fail to develop into blastocysts are in fact aneuploid [8]. Furthermore, even in young women, at least 60% of embryos on day 3 are aneuploid; therefore, blastocyst transfer could improve IVF outcome by eliminating some karyotypically abnormal embryos [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…We recently reported that >90% of embryos that fail to develop into blastocysts are in fact aneuploid [8]. Furthermore, even in young women, at least 60% of embryos on day 3 are aneuploid; therefore, blastocyst transfer could improve IVF outcome by eliminating some karyotypically abnormal embryos [8,9].…”

Section: Introductionmentioning

confidence: 99%

Vitrification of human embryos subjected to blastomere biopsy for pre-implantation genetic screening produces higher survival and pregnancy rates than slow freezing

Keskintepe

Sher

Machnicka

et al. 2009

J Assist Reprod Genet

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Purpose Cryopreservation of blastocysts, especially those subjected to the trauma due to blastomere biopsy for the purposes of pre-implantation genetic screening (PGS), requires significant optimization. Laboratory and clinical outcomes were compared to determine the effect of two different cryopreservation techniques on the development of human pre-implantation embryos that underwent blastomere biopsy and blastocoel drainage prior to cryopreservation. Design Retrospective clinical study. Patient(s) Women who requested cryotransfer of supernumerary blastocysts were analyzed by FISH. Results The main outcome measures were post-thaw survival (SR), pregnancy (PR), and implantation (IR). The SR of slowly frozen blastocysts was 83% compared to 97% for vitrified blastocysts. In 160 cases where biopsied embryos were cryotransferred, the results for slowly frozen versus vitrified blastocysts were: SR (71% vs. 95%), PR (23% vs. 37%), and IR (26% vs. 36%, P<0.05), respectively. Conclusion The results revealed that vitrified blastocysts provided higher SR, PR and IR as compared to slowly frozen counterparts.

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Oocyte karyotyping by comparative genomic hybrydization provides a highly reliable method for selecting “competent” embryos, markedly improving in vitro fertilization outcome: a multiphase study

Cited by 91 publications

References 31 publications

The contribution of mitochondrial function to reproductive aging

The contribution of mitochondrial function to reproductive aging

Elastic and Viscoelastic Characterization of Mouse Oocytes Using Micropipette Indentation

Vitrification of human embryos subjected to blastomere biopsy for pre-implantation genetic screening produces higher survival and pregnancy rates than slow freezing

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