Oocyte maturation failure: a syndrome of bad eggs

Beall, Stephanie; Brenner, Carol A.; Segars, James H.

doi:10.1016/j.fertnstert.2010.02.037

Cited by 96 publications

(73 citation statements)

References 82 publications

(57 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Meiotic resumption is triggered by a surge of the luteinizing hormone (LH), which activates the phosphodiesterase Pde3A to hydrolyze cAMP and to drive entry into meiotic maturation (Sun et al, 2009) as reflected, for example, by the breakdown of the nuclear envelope (also called GV breakdown, or GVBD). Alterations of this pathway often lead to infertility as exemplified by the arrest of oocytes at the GV stage in absence of Pde3a (Beall et al, 2010;Masciarelli et al, 2004). Following GVBD, oocytes condense their chromatin, align chromosomes on the metaphase I (MI) plate by a functional spindle, undergo chromosome segregation and extrude the first polar body (PB), to ultimately become arrested at metaphase II (MII).…”

Section: Introductionmentioning

confidence: 99%

The methyltransferase Setdb1 is essential for meiosis and mitosis in mouse oocytes and early embryos

et al. 2016

View full text Add to dashboard Cite

Oocytes develop the competence for meiosis and early embryogenesis during their growth. Setdb1 is a histone H3 lysine 9 (H3K9) methyltransferase required for post-implantation development and has been implicated in the transcriptional silencing of genes and endogenous retroviral elements (ERVs). To address its role in oogenesis and pre-implantation development, we conditionally deleted Setdb1 in growing oocytes. Loss of Setdb1 expression greatly impaired meiosis. It delayed meiotic resumption, altered the dynamics of chromatin condensation, and impaired kinetochore-spindle interactions, bipolar spindle organization and chromosome segregation in more mature oocytes. The observed phenotypes related to changes in abundance of specific transcripts in mutant oocytes. Setdb1 maternally deficient embryos arrested during pre-implantation development and showed comparable defects during cell cycle progression and in chromosome segregation. Finally, transcriptional profiling data indicate that Setdb1 downregulates rather than silences expression of ERVK and ERVLMaLR retrotransposons and associated chimearic transcripts during oogenesis. Our results identify Setdb1 as a newly discovered meiotic and embryonic competence factor safeguarding genome integrity at the onset of life.

show abstract

Section: Introductionmentioning

confidence: 99%

The methyltransferase Setdb1 is essential for meiosis and mitosis in mouse oocytes and early embryos

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Visual assessment of the compactness of the cumulus investment as well as the homogeneity of the ooplasm is routinely used for selecting the more competent oocytes in ART programs [3]. Although such morphological characteristics are practically used in determining the quality of oocytes, their overall accuracy for assessing developmental potential are poor and therefore the predictive value of these criteria are more likely controversial [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…As stated above, the BCB test is depend on the capability of G6PDH to convert the dye from blue to a colorless state. Due to sufficient concentrations of the enzyme in oocytes undergoing growth, the dye is reduced to colorless, while fully-grown oocytes remain blue due to the low amount of cytoplasmic G6PDH-activity [4].…”

Section: Introductionmentioning

confidence: 99%

Intracellular glutathione content, developmental competence and expression of apoptosis-related genes associated with G6PDH-activity in goat oocyte

Abazari-Kia

Mohammadi‐Sangcheshmeh

Dehghani-Mohammadabadi

et al. 2013

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To associate glucose-6-phosphate dehydrogenase (G6PDH) activity in goat oocytes with intracellular glutathione (GSH) content, meiotic competence, developmental potential, and relative abundance of Bax and Bcl-2 genes transcripts. Methods Goat oocytes were exposed to brilliant cresyl blue (BCB) staining test and categorized into BCB + (bluecytoplasm), and BCB − (colorless-cytoplasm) groups. A group of oocytes were not exposed to BCB test and was considered as a control group. After maturation in vitro, a group of oocytes were used for determination of nuclear status and intracellular GSH content while another group was subjected to parthenogenetic activation followed by in vitro embryo culture.Results We found that BCB + oocytes not only yielded higher rate of maturation, but also showed an increased level of intracellular GSH content than BCB − and control oocytes.Furthermore, BCB + oocytes produced more blastocysts than BCB − and control oocytes. Our data revealed that the expression of anti-apoptotic (Bcl-2) and pro-apoptotic (Bax) genes were interacted with G6PDH-activity in mature oocyte, their surrounding cumulus cells, and blastocyst-stage embryos.Conclusions The results of this study demonstrate that selection of goat oocytes based on G6PDH-activity through the BCB test improves their developmental competence, increases intracellular GSH content, and affects the expression of the apoptosis-related genes.

show abstract

“…Degradation of maternal proteins and transcripts is carried out rapidly in many organisms and plays a critical role in oocyte maturation. Most protein degradation is regulated by the ubiquitin-proteasome system and any impairment in degradation results in the failure of oocyte maturation (DeRenzo & Seydoux 2004, Huo et al 2004, Ryu et al 2008, Beall et al 2010. UCHL1 is essential for maintaining the ubiquitin-proteasome system and is abundantly expressed in mouse ova (Sekiguchi et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Effects of ubiquitin C-terminal hydrolase L1 deficiency on mouse ova

et al. 2012

View full text Add to dashboard Cite

Maternal proteins are rapidly degraded by the ubiquitin-proteasome system during oocyte maturation in mice. Ubiquitin C-terminal hydrolase L1 (UCHL1) is highly and specifically expressed in mouse ova and is involved in the polyspermy block. However, the role of UCHL1 in the underlying mechanism of polyspermy block is poorly understood. To address this issue, we performed a comprehensive proteomic analysis to identify maternal proteins that were relevant to the role of UCHL1 in mouse ova using UCHL1-deficient gad. Furthermore, we assessed morphological features in gad mouse ova using transmission electron microscopy. NACHT, LRR, and PYD domain-containing (NALP) family proteins and endoplasmic reticulum (ER) chaperones were identified by proteomic analysis. We also found that the 'maternal antigen that embryos require' (NLRP5 (MATER)) protein level increased significantly in gad mouse ova compared with that in wild-type mice. In an ultrastructural study, gad mouse ova contained less ER in the cortex than in wild-type mice. These results provide new insights into the role of UCHL1 in the mechanism of polyspermy block in mouse ova.

show abstract

Oocyte maturation failure: a syndrome of bad eggs

Cited by 96 publications

References 82 publications

The methyltransferase Setdb1 is essential for meiosis and mitosis in mouse oocytes and early embryos

The methyltransferase Setdb1 is essential for meiosis and mitosis in mouse oocytes and early embryos

Intracellular glutathione content, developmental competence and expression of apoptosis-related genes associated with G6PDH-activity in goat oocyte

Effects of ubiquitin C-terminal hydrolase L1 deficiency on mouse ova

Contact Info

Product

Resources

About