Oocyte morphology correlates with embryo quality and pregnancy rate after intracytoplasmic sperm injection

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Purpose The aim of the study was to evaluate whether the presence Antimullerian hormone (AMH) and Antimullerian hormone type II receptor (AMHRII) single nucleotide polymorphisms (SNPs) Ile 49 Ser and -482A>G respectively are related to the assisted reproduction outcome. Methods A prospective cross-sectional observational study was conducted in order to assess the distribution of AMH and AMHRII SNPs in two cohorts, one of healthy women (N=100) and the control group and the IVF/ICSI group (N=151) consisted of women undergoing IVF/ICSI treatment for infertility. Furthermore, a prospective longitudinal observational study was performed on the latter group to assess possible associations of these SNPs with patients' characteristics and controlled ovarian stimulation (COS) and pregnancy outcome. Results Among non-carriers of the AMH (Ile 49 Ser) polymorphism, basal FSH levels were lower in those with more than two of previous IVF attempts and fertilization rate was statistically higher in those with peak serum E2 levels below 1500 pg/ml, whereas among non-carriers of the AMHRII (−482 A>G) polymorphism, number of follicles was higher in those with more than two previous IVF attempts and total dose of gonadotropins was lower in those with peak serum E2 levels above 1500 pg/ml. Conclusions There was evidence that in specific subgroups of women undergoing IVF/ICSI, AMH and AMHRII SNPs may be related to patients' characteristics and controlled ovarian stimulation and pregnancy outcome and thus may provide a means for the prediction of ovarian response in specific subgroups of women entering an IVF/ICSI program.

Section: Cos and Ivf/icsimentioning

confidence: 99%

Single nucleotide polymorphisms in the Anti-Müllerian hormone (AMH Ile49Ser) and Anti-Müllerian hormone type II receptor (AMHRII −482 A>G) as genetic markers in assisted reproduction technology

Karagiorga

Partsinevelos

Mavrogianni

et al. 2014

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“…Human metaphase II oocytes have distinct morphological characteristics that may be indicative of quality and therefore used to predict implantation potential [21,[36][37][38][39][40][41][42][43]. For example, regular patterns of granularity have been observed in the oocyte cytoplasm and it has been suggested that these patterns correlate with oocyte quality [44,45].…”

Section: Introductionmentioning

confidence: 99%

An oocyte score for use in assisted reproduction

Wilding¹,

Matteo

D’Andretti³

et al. 2007

Purpose In this work, we describe a system for the morphological scoring of human oocytes prior to fertilisation and use this system to test whether oocyte morphology is an indicator of fertilisation, embryo development and implantation potential. Methods The study is a prospective trial of the use of oocyte morphological scores in 822 patients undergoing their first cycle of ICSI. Analyses of oocytes were performed prior to ICSI procedures and the scores compared with fertilisation rates, embryo quality and clinical results.Results 'Top quality' oocytes had a significantly higher level of fertilisation (96%) as compared to low scoring oocytes (25.6%). Where top quality oocytes formed top quality embryos, we noted a clinical success rate of 63.4%. Conclusions Clinical success rates were increased in cases where top quality oocytes formed top quality embryos after ICSI. The analysis of oocyte morphology may represent a positive selection feature during ICSI.

“…Estrogens affect the oocyte maturation, providing an optimal oocyte cytoplasm and oolemma maturation and stand as an important factor that determines the quality of oocyte [3]. Regarding the infertility management of women undergoing IVF/ICSI, peak E2 on the day of hCG administration is a marker of embryo quality [19]. Equally important is the role of estrogen receptors, which mediate the estrogen signal onwards.…”

Section: Discussionmentioning

confidence: 99%

“…ICSI was performed only in mature oocytes which had extruded the first polar body (metaphase II). The ICSI procedure was performed following conventional techniques [19].…”

Section: Cos and Ivf/icsimentioning

confidence: 99%

“…Experiments with estrogen receptor (ER) knockout mice have shown that in ERα knockout mice folliculogenesis is arrested after the pre-antral state, while in ERβ knockout mice folliculogenesis is diminished [7,14,28]. Clinically, estrogen levels are used to monitor the ovarian response to stimulation and studies have shown that serum estradiol levels on the day of human chorionic gonadotropin (hCG) administration correlate directly to embryo quality and pregnancy rates [19], although the role of serum estrogen levels in ART is controversial [15]. Pharmacogenetic studies have been conducted in order to evaluate the role of hormonal receptors, including ER in COS. Estrogens mediate their actions through two subtypes of nuclear receptors, ERα and ERβ, which are encoded by ESR1 and ESR2 genes, present on distinct chromosomes (locus 6q25.1 and locus 14q23-24.1, respectively).…”

Section: Introductionmentioning

confidence: 99%

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Do estrogen receptor alpha polymorphisms have any impact on the outcome in an ART program?

Anagnostou

Malamas

Mavrogianni

et al. 2013

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Purpose To investigate two of the most studied estrogen receptor alpha polymorphisms (PvuII and XbaI) in combination, in order to evaluate their impact on an ART program outcome. Methods 203 normally ovulating women who underwent IVF or ICSI treatment were genotyped for PvuII and XbaI polymorphisms in ESR1 intron 1 using Real-Time PCR. The relationship between the presence of polymorphic alleles and the ovulation induction parameters and outcome was examined. Results Women were grouped according to the number of polymorphic alleles they carried in two groups (0-2 versus 3-4 polymorphic alleles). The presence of 3 or more polymorphic alleles was associated with significantly lower E2 levels on the day of hCG administration and a significantly lower rate of good quality embryos. Conclusion There is an association between ESR1 polymorphisms and some ART parameters such as the level of E2 on the day of hCG administration and the quality of the embryos. These results underline the importance of ESR1 as a candidate gene for the prediction of ovarian response to IVF/ICSI protocols. Future research work concerning several more genes is necessary for a better evaluation of patients before entering an IVF/ICSI program.