Oogenetic and zygotic gene expression directing early bovine embryogenesis: A review

Sousa, Paul A. De; Watson, Andrew J.; Schultz, Gilbert A.; Bilodeau‐Goeseels, Sylvie

doi:10.1002/(sici)1098-2795(199809)51:1<112::aid-mrd14>3.0.co;2-9

Cited by 65 publications

(33 citation statements)

References 137 publications

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“…The initial development of mammalian embryos is governed by gene transcripts and polypeptides produced by, and stored in, the oocytes during oogenesis [43]. However, following 1 to 3 cleavage divisions, expression of portions of the embryonic genome starts, and concomitantly, the maternally derived transcripts and proteins are gradually degraded [44][45][46][47]. This transition from maternal to embryonic gene expression is generally a gradual phenomenon.…”

Section: Discussionmentioning

confidence: 99%

Development of a Noninvasive Monitoring System for Evaluation of Oct-3/4 Promoter Status in Miniature Pig Somatic Cell Nuclear Transfer Embryos

Miyoshi

Mori

Mizobe

et al. 2009

Journal of Reproduction and Development

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Abstract. The present study was carried out to develop a noninvasive monitoring system for evaluation of Oct-3/4 promoter gene status in miniature pig somatic cell nuclear transfer (SCNT) embryos during in vitro development. Miniature pig fetal fibroblasts (MPFFs) were transfected with a gene construct consisting of two expression units, a mouse Oct-3/4 promoter-driven enhanced green fluorescent protein (EGFP) gene (EGFP expression only detected in Oct-3/4-expressing cells) and a neomycin resistance gene. After neomycin selection, MPFFs that did not express EGFP were fused with enucleated pig oocytes, cultured in vitro and assessed for EGFP expression. EGFP expression was detectable in all morulae (at 4-6 days of culture) and 50.0% of blastocysts (at 5-6 days of culture), whereas none of the 1-cell to 16-cell embryos at 1-5 days of culture expressed EGFP. On the other hand, EGFP expression was not maintained in all blastocysts at 7 days of culture. The reactivity with anti-Oct-3/4 antibodies also peaked from the morula to blastocyst stages at 5 days of culture. The results showed that reactivation of the Oct-3/4 promoter gene of donor nuclei occurs in the morula to blastocyst stages at 4-6 days after SCNT and that this noninvasive monitoring system using Oct-3/4 promoter-driven EGFP gene would be useful for evaluation of the reprogramming status of donor nuclei. Key words: Enhanced green fluorescent protein, In vitro development, Nuclear transfer, Oct-3/4 promoter, Reprogramming (J. Reprod. Dev. 55: [661][662][663][664][665][666][667][668][669] 2009) uccessful production of cloned animals using somatic cell nuclear transfer (SCNT) has been reported in several mammalian species [1][2][3][4][5][6][7][8][9][10][11][12]. However, the low cloning efficiency associated with development of SCNT embryos to offspring remains the major obstacle to widespread use of this technology in a number of animal science and biomedical applications. A method for evaluating the developmental ability of SCNT embryos before being transferred into recipient females should be explored to optimize the procedures of SCNT and overcome the low cloning efficiency. Evaluation of the in vitro developmental ability of an SCNT embryo to the blastocyst stage would be considered one of the methods for predicting its in vivo development, but it has been found that this does not often assure successful development to term of SCNT embryos [13]. After SCNT, the gene expression pattern in somatic cells is reprogrammed to mimic that in preimplantation embryos [14]. However, there is a difference in gene expression pattern between SCNT embryos and in vitro-fertilized embryos, suggesting that the low efficiency of SCNT is associated with incomplete reprogramming in the donor nuclei transferred into recipient oocytes [15]. Therefore, evaluation of the reprogramming level in the donor nuclei appears to be most essential for predicting the in vivo developmental ability of SCNT embryos.Oct-3/4 is a transcription factor of the Pit-Oct-Unc family [16,17], and...

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Section: Discussionmentioning

confidence: 99%

Development of a Noninvasive Monitoring System for Evaluation of Oct-3/4 Promoter Status in Miniature Pig Somatic Cell Nuclear Transfer Embryos

Miyoshi

Mori

Mizobe

et al. 2009

Journal of Reproduction and Development

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“…After one to several cleavage divisions (depending upon the species), genetic control of development comes predominantly under the embryo's control as oogenetic gene products decline, and embryonic transcription begins (19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29). The transition from oogenetic to embryonic control of development or maternal zygotic transition (MZT) varies in its timing between species (4,22,30).…”

Section: Oogenetic To Embryonic Transitionmentioning

confidence: 99%

“…Since this topic is the subject of several reviews (for example 19,22,30,34,35) including a chapter in this issue only the principal components will be briefly summarized. Mouse embryos cultured from the one-cell stage in the presence of transcriptional inhibitors do not develop beyond the two-cell stage, indicating that new transcription from the zygote genome is, necessary for development beyond this point (19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29). During this transition, the majority of maternal mRNA molecules accumulated during oogenesis are degraded and replaced by new mRNA molecules (20-24, 28, 36).…”

Section: Oogenetic To Embryonic Transitionmentioning

confidence: 99%

Regulation of blastocyst formation

Watson¹

2001

Front Biosci

115

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“…In bovine oocytes, genes for some transcripts have been reported to be specifically or highly expressed in oocytes from large (≥5 mm) but not in small follicles (≤2-mm) (Robert et al, 2000;Donnison and Pfeffer, 2004). The transcriptional activity of bovine oocytes drops dramatically after the follicle has grown to 3 mm and remains nearly quiescent thereafter (Fair et al, 1995;De Sousa et al, 1998). It was therefore suggested that follicles smaller than 3 mm have not yet attained the full RNA complement required for developing beyond the stage or time when embryonic transcription is initiated.…”

Section: Effect Of Follicle Status On Oocyte Ivm In Rhesus Monkeysmentioning

confidence: 99%

Effects of in vitro maturation of monkey oocytes on their developmental capacity

Zheng

2007

Animal Reproduction Science

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The study of in vitro maturation (IVM) of rhesus monkey oocytes has important implications for biomedical research and human infertility treatment. In vitro-matured rhesus monkey oocytes show much less developmental potential than IVM oocytes of other species. Since about 1980 when rhesus monkey IVM, in vitro fertilization (IVF) and in vitro embryo culture (IVC) systems were established, numerous efforts have been made to improve the developmental competence of oocytes and to understand the mechanisms regulating oocyte maturation. This review describes recent progress in this area, particularly the effects of factors such as steroid hormones, energy substrates, amino acids, ovarian follicle status, maternal age and breeding season on the developmental competence, gene expression patterns and genome integrity of rhesus IVM oocytes.

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Oogenetic and zygotic gene expression directing early bovine embryogenesis: A review

Cited by 65 publications

References 137 publications

Development of a Noninvasive Monitoring System for Evaluation of Oct-3/4 Promoter Status in Miniature Pig Somatic Cell Nuclear Transfer Embryos

Development of a Noninvasive Monitoring System for Evaluation of Oct-3/4 Promoter Status in Miniature Pig Somatic Cell Nuclear Transfer Embryos

Regulation of blastocyst formation

Effects of in vitro maturation of monkey oocytes on their developmental capacity

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