Ooplasmic transfer in mature human oocytes

Cohen, Jacques; Scott, Richard T.; Alikani, Mina; Schimmel, Tim; Munné, Santiago; Levron, Jacob; Wu, Lizi; Brenner, Carol A.; Warner, Carol M.; Willadsen, S. M.

doi:10.1093/molehr/4.3.269

Cited by 308 publications

(209 citation statements)

References 44 publications

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“…Fully mature cytoplasm of the oocyte provides the embryo with a battery of maternal mRNAs, proteins, and mitochondria, which are related to oocyte development competence and which may contribute to apoptotic pathways but also to defense mechanisms against apoptosis (Fair et al 1995, Otoi et al 1997, Stojkovic et al 2001. Microinjection of healthy cytoplasm protected oxidative-stressed zygotes from apoptotic death in mice (Liu & Keefe 2000) and improved fertility in older patients with compromised embryos in humans (Cohen et al 1998, Barritt et al 2001. Porcine studies have also shown that mitochondrial activity increases during meiotic progression and cumulus expansion (Thorner et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Temporal detection of caspase-3 and -7 in bovine in vitro produced embryos of different developmental capacity

Vandaele

Mateusen²,

Maes³

et al. 2007

Reproduction

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Embryo quality is most frequently evaluated at the blastocyst stage, although quality parameters further back along the developmental axis, such as early developmental kinetics or oocyte quality, can be equally valuable. Despite the fact that previous studies in bovine have linked oocyte diameter and early developmental kinetics with blastocyst formation and viability, their relation with the incidence of apoptosis during embryo development remains relatively unexplored. Therefore, we related noninvasive parameters of oocyte and embryo quality, such as embryo kinetics, embryo morphology, and oocyte diameter, to the incidence of apoptosis throughout embryo development using fluorescent detection of active caspase-3 and -7. First, bovine in vitro embryos were selected according to developmental kinetics and morphology at four set times during culture and subjected to fluorescent detection of active caspase-3 and -7. Caspase activity was significantly higher in slow developing embryos in comparison with fast cleavers (P!0.05), but was not related to embryo morphology. Second, bovine oocytes were divided into three groups on the basis of oocyte diameter and the resulting embryos were used for staining at the same four set times. Caspase activity was significantly higher in embryos derived from growing oocytes compared with those of fully grown oocytes at 45, 80, and 117 hours post-insemination (hpi; P!0.05), but not at 168 hpi. Reproduction (2007) 133 709-718

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Section: Discussionmentioning

confidence: 99%

Temporal detection of caspase-3 and -7 in bovine in vitro produced embryos of different developmental capacity

Vandaele

Mateusen²,

Maes³

et al. 2007

Reproduction

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“…Oxidative stress can also influence the oocyte spindle assembly (Tarín, 1996), because oxidative stress may trigger mPT pore opening and decrease ATP content in oocytes (Zhang et al, 2006). It is therefore not surprising that injection of cytoplasm may improve embryo quality and support the embryo development (Cohen et al, 1998).…”

Section: Mitochondria and Meiosis In Oocytesmentioning

confidence: 99%

Mitochondrial functions on oocytes and preimplantation embryos

Wang

Zou

et al. 2009

J. Zhejiang Univ. Sci. B

138

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Oocyte quality has long been considered as a main limiting factor for in vitro fertilization (IVF). In the past decade, extensive observations demonstrated that the mitochondrion plays a vital role in the oocyte cytoplasm, for it can provide adenosine triphosphate (ATP) for fertilization and preimplantation embryo development and also act as stores of intracellular calcium and proapoptotic factors. During the oocyte maturation, mitochondria are characterized by distinct changes of their distribution pattern from being homogeneous to heterogeneous, which is correlated with the cumulus apoptosis. Oocyte quality decreases with the increasing maternal age. Recent studies have shown that low quality oocytes have some age-related dysfunctions, which include the decrease in mitochondrial membrane potential, increase of mitochondrial DNA (mtDNA) damages, chromosomal aneuploidies, the incidence of apoptosis, and changes in mitochondrial gene expression. All these dysfunctions may cause a high level of developmental retardation and arrest of preimplantation embryos. It has been suggested that these mitochondrial changes may arise from excessive reactive oxygen species (ROS) that is closely associated with the oxidative energy production or calcium overload, which may trigger permeability transition pore opening and subsequent apoptosis. Therefore, mitochondria can be seen as signs for oocyte quality evaluation, and it is possible that the oocyte quality can be improved by enhancing the physical function of mitochondria. Here we reviewed recent advances in mitochondrial functions on oocytes.

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“…Many studies were performed in order to ascertain factors that af-fect the fertilization rate, where the fertilization rate and embryo quality have been described in conjunction with polar body orientation or meiotic spindle position. The M-II spindle is located at the periphery of the ooplasm, subjacent to the first polar body (2). Therefore, during the ICSI procedure sperm injection at the M-II spindle area should be avoided, and a six or twelve o'clock position in relation to the polar body chosen (3,4).…”

Section: Introductionmentioning

confidence: 99%

Sperm retention site and its influence on cleavage rate and early development following intracytoplasmic sperm injection

Yanaihara

Iwasaki

Negishi

et al. 2006

J Assist Reprod Genet

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Background: Intracytoplasmic sperm injection (ICSI) has risen to the forefront of reproductive technology. In the present study, the location of the sperm injection was noted, and a prospective study was conducted to evaluate the effect of the sperm retention site on cleavage rates and embryo quality after ICSI. Methods: This study involved 336 ICSI patients (age 27-44; average 37.4) where 1545 oocytes were observed. An oocyte was divided into nine sites and the sperm retention site was observed microscopically after injection. The polar body was placed at either the twelve or six o'clock position. The injection pipette was introduced at the three o'clock position and oolemma rupture was ascertained by mild suction. The main outcome measures were the relationship of sperm remaining in position in the oocyte to fertilization rate and embryo quality. Results: When the injection pipette was introduced at the three o'clock position, about 80% of the sperm remained in the center or left of center. The fertilization rate was significantly lower (p < 0.05) when the sperm remained near the site of introduction. Embryo quality was not significantly affected by the sperm retention site. Conclusions: About 12-14% of the spermatozoa remained near the introducing position, and in these cases the fertilization rate was low. However, once fertilization occurred, the sperm retention site had minimal impact on embryo quality. Injecting sperm near the spindle site may improve embryo quality.

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Ooplasmic transfer in mature human oocytes

Cited by 308 publications

References 44 publications

Temporal detection of caspase-3 and -7 in bovine in vitro produced embryos of different developmental capacity

Temporal detection of caspase-3 and -7 in bovine in vitro produced embryos of different developmental capacity

Mitochondrial functions on oocytes and preimplantation embryos

Sperm retention site and its influence on cleavage rate and early development following intracytoplasmic sperm injection

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