opa: A novel family of transcribed repeats shared by the Notch locus and other developmentally regulated loci in D. melanogaster

Wharton, Kristi A.; Yedvobnick, Barry; Finnerty, Victoria; Artavanis‐Tsakonas, Spyros

doi:10.1016/0092-8674(85)90308-3

Cited by 393 publications

(163 citation statements)

References 29 publications

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“…Aside from the zinc finger motifs (which we propose to be amino acids 876 to 896, 904 to 924, 932 to 953, and 971 to 992 relative to the first methionine codon in the longest ORF), the 1,028-amino-acid putative Ovo protein sequence has no significant homologies in the nonredundant protein sequence databases. The N-terminal putative activation domain contains regions of unusual amino acid composition, including homopolymeric runs of glycine, serine, alanine, asparagine, and glutamine, characteristic of the coding potential of opa repeats (41). In addition, the putative activation domain contains acidic regions (e.g., amino acids 180 to 200, 275 to 290, and 600 to 640), the final one of which is coded by the ovo-specific exon 2 extension.…”

Section: Resultsmentioning

confidence: 99%

Multiple products from the shavenbaby-ovo gene region of Drosophila melanogaster: relationship to genetic complexity.

Garfinkel¹,

Wang²,

Liang³

et al. 1994

Mol. Cell. Biol.

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The Drosophila melanogaster shavenbaby (svb)-ovo gene region is a complex locus, containing two distinct but comutable genetic functions. ovo is required for survival and differentiation of female germ line cells and plays a role in germ line sex determination. In contrast, svb is required in both male and female embryos for the production of epidermal locomotor and sensory structures. Sequences required for the two genetic functions are partially overlapping. ovo corresponds to a previously described germ line-dependent 5.0-kb poly(A)+ mRNA that first appears in the germarium and accumulates in nurse cells during oogenesis. The 5.0-kb mRNA is stored in the egg, but it is rapidly lost in the embryos except for its continued presence in the germ line precursor pole cells. The ovo mRNA predicts a 1,028-amino-acid 110.6-kDa protein homologous with transcription factors. We have identified an embryonic mRNA, 7.1 kb in length, that contains exons partially overlapping those of the 5.0-kb poly(A)+ mRNA. The spatial distribution of this newly discovered transcript during midembryogenesis suggests that it corresponds to the svb function. The arrangement of exons common to the 5.0-and 7.1-kb mRNAs suggests that the Ovo and Svb proteins share DNA-binding specificity conferred by four Cys2-His2 zinc finger motifs but differ functionally in their capacity to interact with other components of the transcription machinery.The central role of Sex-lethal (Sxl) for sex determination in the somatic tissues of Drosophila melanogaster has been elegantly worked out (reviewed in reference 9). However, the mechanisms active in the germ line differ in three major ways from those operating in somatic sex determination. First, although Sxl is required in the germ line, it is not active in the germ line precursor cells of early embryos (4) when sex-specific germ line functions first appear. Second, the activation of Sxl in the germ line does not require the same X-linked genes as required for its activation in embryonic somatic cells (14, 37), nor are the downstream genes required in the germ line (reviewed in references 28 and 36). Third, germ line sex determination is not autonomous but depends upon proper somatic cell-germ line interactions (38). Thus, it is necessary to identify the genes required by germ line cells for the assessment of sex identity and to determine the biochemical pathways by which they operate.A key gene for sex-dimorphic germ line functions is ovo. Recessive, apparently null, alleles cause female-specific sterility due to the loss of germ cells in the embryo (27 ovo alleles to suppress partially P-M hybrid dysgenesisinduced female germ line cell death (39). Thus, ovo appears to play a prominent role in the early female-specific establishment of the germ line as well as in subsequent oocyte development and differentiation.Genomic DNA sequences required for ovo function are genetically overlapping and interdigitated with the genomic DNA sequences required for the shavenbaby (svb) function (13,22). svb is known to aff...

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Section: Resultsmentioning

confidence: 99%

Multiple products from the shavenbaby-ovo gene region of Drosophila melanogaster: relationship to genetic complexity.

Garfinkel¹,

Wang²,

Liang³

et al. 1994

Mol. Cell. Biol.

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“…The inserts are 391 and 505 bp long and do not overlap ( Figure 1C). They do not contain homeo box sequences, but do exhibit some homology to sequences present at several developmentally regulated loci (Wharton et al, 1985).…”

Section: Expression Of Engrailed Fusion Proteinsmentioning

confidence: 99%

Development of embryonic pattern in D. melanogaster as revealed by accumulation of the nuclear engrailed protein

DiNardo

1985

Cell

397

193

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Summaryengrailed is required to establish and maintain developmental compartments within each segment of the fly. To understand the role of the engrailed protein in this process, we have raised antibodies against engrailed and have visualized an engrailed protein in embryos by indirect immunofluorescence. The protein accumulates in the nucleus, supporting the notion that engrailed is a regulatory factor. The first pattern of expression is in alternating segments followed by expression in every segment, suggesting that engrailed may be responding to pair-rule segmentation gene products. Overall, engrailed protein levels peak in areas undergoing morphogenesis. Finally, the complex final form of the head and terminalia derive from earlier simple subdivision of these areas into developmental fields by engrailed.

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“…Por exemplo, seqüências com 20 ou mais resíduos de poliglutamina já foram referidas em muitos fatores de transcrição, incluindo a proteína NOTCH em drosófila (44), o fator de transcrição I1D na espécie humana (45,46), a GAL4 em leveduras (43) e o AR de diferentes organismos (20). Repetições de trinucleotídeos são descritas também em genes de algumas doenças hereditárias.…”

Section: O Receptor De Andrógenosunclassified

Fatores hormonais e genéticos na próstata normal e neoplásica

Carvalho-Salles¹,

Tajara²

1999

Arq Bras Endocrinol Metab

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Recently, a great deal of attention has focused on the growth regulatory effects of androgens in prostate cells. This has also resulted in much interest in the role of these steroid hormones in the development and maintenance of both normal and neoplastic prostate. However, it has not been possible to identify the exact relationship between androgenic hormone levels and the risk of this disease. The hormone-androgen receptor complex, by association with the DNA androgen response elements, specifically promotes the growth of the prostate gland. It has been recognized that there is a close relationship between the androgen mediated signaling pathway and those promoted by peptide growth factors. More insights are being gained into these complex interactions, some of which may lead to novel therapeutic maneuvers. (Arq Bras Endocrinol Metab 1999;43/3: 177-185).Keywords: Prostate; Prostatic neoplasia; Androgens; Growth factors; Therapy. CONSIDERAÇÕES GERAISA PRÓSTATA É UM ÓRGÃO FIRME, de consistência fibroclástica, que pesa cerca de 20 gramas e apresenta diâmetros máximos de 40 mm. Está localizada na base da bexiga urinaria e, do ponto de vista anatômico, pode ser dividida em lobos laterais, anterior, posterior e mediano. Essa divisão somente é nítida no período embrionário e, no órgão adulto, o limite entre os lobos é impreciso, não existindo septos de tecido conjuntivo que os separem. Histologicamente, apresenta dois tipos glandulares, tubuloacinosas compostas e ramificadas, cuja secreção tem como principal função manter

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opa: A novel family of transcribed repeats shared by the Notch locus and other developmentally regulated loci in D. melanogaster

Cited by 393 publications

References 29 publications

Multiple products from the shavenbaby-ovo gene region of Drosophila melanogaster: relationship to genetic complexity.

Multiple products from the shavenbaby-ovo gene region of Drosophila melanogaster: relationship to genetic complexity.

Development of embryonic pattern in D. melanogaster as revealed by accumulation of the nuclear engrailed protein

Fatores hormonais e genéticos na próstata normal e neoplásica

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