Open field inoculation with PGPR as a strategy to manage fertilization of ancient Triticum genotypes

Pagnani, Giancarlo; Galieni, Angelica; Stagnari, Fabio; Pellegrini, Marika; Gallo, Maddalena Del; Pisante, Michele

doi:10.1007/s00374-019-01407-1

Cited by 47 publications

(28 citation statements)

References 87 publications

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“…The main agronomic result obtained in this study was the greater nitrogen uptake in inoculated plants, although this did not translate into significant gains in grain yield, and no effects on grain quality in terms of gluten content were observed, in contrast with the findings of some authors which tested the effects of PGPR inoculation in ancient Triticum varieties (Pagnani et al, 2020). Accumulation of gluten proteins is a complex process involving spatial and temporal regulation.…”

Section: Treatmentcontrasting

confidence: 82%

“…is related to the interaction with the resident microbial community, and the possible mechanisms of interaction with the wheat plants. Using this application method, we expect endophytic PGPR to colonize the root surface and intercellularly colonize the internal plant tissues of different plant organs, and AMF to colonize the plant roots starting with the first rootlet of the germinating seeds, thereby contributing to plant nutrition and growth (Lodewyckx et al, 2002;Smith and Read, 2008;Pagnani et al, 2020). Indeed, efficient colonization of root tissues by PGPR bacteria included in TN, as well as by Rhizophagus irregularis contained in R-N and R-PK, has been already documented by electron microscopy (ESEM) in our previous studies (Dal Cortivo et al, 2017;Dal Cortivo et al, 2018).…”

Section: Treatmentmentioning

confidence: 99%

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Effects of Seed-Applied Biofertilizers on Rhizosphere Biodiversity and Growth of Common Wheat (Triticum aestivum L.) in the Field

et al. 2020

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In order to reduce chemical fertilization and improve the sustainability of common wheat (Triticum aestivum L.) cultivation, maintaining at the same time high production and quality standards, this study investigated the effects of three commercial biofertilizers on rhizosphere bacterial biomass, biodiversity and enzymatic activity, and on plant growth and grain yield in a field trial. The wheat seeds were inoculated with the following aiding microrganisms: (i) a bacterial consortium (Azospirillum spp. + Azoarcus spp. + Azorhizobium spp.); and two mycorrhizal fungal-bacterial consortia, viz. (ii) Rhizophagus irregularis + Azotobacter vinelandii, and (iii) R. irregularis + Bacillus megaterium + Frateuria aurantia, and comparisons were made with noninoculated controls. We demonstrate that all the biofertilizers significantly enhanced plant growth and nitrogen accumulation during stem elongation and heading, but this was translated into only small grain yield gains (+1%-4% vs controls). The total gluten content of the flour was not affected, but in general biofertilization significantly upregulated two high-quality protein subunits, i.e., the 81 kDa high-molecular-weight glutenin subunit and the 43.6 kDa low-molecular-weight glutenin subunit. These effects were associated with increases in the rhizosphere microbial biomass and the activity of enzymes such as b-glucosidase, a-mannosidase, bmannosidase, and xylosidase, which are involved in organic matter decomposition, particularly when Rhizophagus irregularis was included as inoculant. No changes in microbial biodiversity were observed. Our results suggest that seed-applied biofertilizers may be effectively exploited in sustainable wheat cultivation without altering the biodiversity of the resident microbiome, but attention should be paid to the composition of the microbial consortia in order to maximize their benefits in crop cultivation.

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Section: Treatmentcontrasting

confidence: 82%

Section: Treatmentmentioning

confidence: 99%

Effects of Seed-Applied Biofertilizers on Rhizosphere Biodiversity and Growth of Common Wheat (Triticum aestivum L.) in the Field

et al. 2020

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“…Seeds were sterilized following the procedure previously described [ 24 ]. Sterilized seeds were inoculated with the 10 10 mL −1 bacterial suspension for 20 min and allowed us to dry overnight at 30 °C.…”

Section: Methodsmentioning

confidence: 99%

“…The production of bacterial consortia, with the combination of two or more strains with PGP characteristics, can increase the effectiveness of the inoculum [ 19 , 20 ]. The combination of A. brasilense , B. ambifaria , G. diazotrophicus, and H. seropedicae strains has already shown good biostimulation effects on Lycopersicon esculentum L. [ 21 ] Cannabis sativa L. [ 22 ] Artemisia eriantha Ten [ 23 ], and ancient Triticum genotypes [ 24 ]. In the present work, we hypothesised that this PGPB consortium could positively affect the growth, development, yield, and quality traits of Allium cepa L. crops.…”

Section: Introductionmentioning

confidence: 99%

Allium cepa L. Inoculation with a Consortium of Plant Growth-Promoting Bacteria: Effects on Plants, Soil, and the Autochthonous Microbial Community

Pellegrini

Spera²,

Ercole

et al. 2021

Microorganisms

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The present work was aimed at investigating the effects of a four bacterial strain consortium—Azospirillum brasilense, Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae, and Burkholderia ambifaria—on Allium cepa L. and on soil health. The bacterial consortium was inoculated on seeds of two different onion varieties; inoculated and Control seeds (treated with autoclaved inoculum) were sown in an open-field and followed until harvest. Plant growth development parameters, as well as soil physico–chemical and molecular profiles (DNA extraction and 16S community sequencing on the Mi-Seq Illumina platform), were investigated. The results showed a positive influence of bacterial application on plant growth, with increased plant height (+18%), total chlorophylls (+42%), crop yields (+13%), and bulb dry matter (+3%) with respect to the Control. The differences between Control and treatments were also underlined in the bulb extracts in terms of total phenolic contents (+25%) and antioxidant activities (+20%). Soil fertility and microbial community structure and diversity were also positively affected by the bacterial inoculum. At harvest, the soil with the presence of the bacterial consortium showed an increase in total organic carbon, organic matter, and available phosphorus, as well as higher concentrations of nutrients than the Control. The ecological indexes calculated from the molecular profiles showed that community diversity was positively affected by the bacterial treatment. The present work showed the effective use of plant growth-promoting bacteria as a valid fertilization strategy to improve yield in productive landscapes whilst safeguarding soil biodiversity.

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“…In this work we investigated the biostimulant effectiveness of a seed inoculation with a bacterial consortium formed by Azospirillum brasilense Cd, Burkholderia ambifaria PHP7, Gluconacetobacter diazotrophicus Pal5 and Herbaspirillum seropedicae Z67. The consortium of these strains already demonstrated good biostimulant effects on Lycopersicon esculentum L. [3] Cannabis sativa L. [4] Artemisia eriantha Ten [5] and ancient Triticum genotypes [6].…”

Section: Introductionmentioning

confidence: 99%

<em>Allium cepa </em>L. <em>s</em>eed inoculation with a consortium of plant growth-promoting bacteria: effects on plant growth and development and soil fertility status and microbial community

Pellegrini

Spera²,

Ercole

et al. 2020

Proceedings of 1st International Electronic Conference on Microbiology

Self Cite

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The present work was aimed at investigating the effects of a four strains consortium-Azospirillum brasilense, Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae, and Burkholderia ambifaria-on crops of Allium cepa L. and its soil health. The bacterial consortium was inoculated on seeds of two different onion varieties; inoculated seeds and control ones (treated with autoclaved inoculum) were sown in open-field and followed until harvest. Plant growth development parameters, as well as soil physico-chemical and molecular profiles (DNA extraction and 16S community sequencing on the Mi-Seq Illumina platform), were investigated. The results showed a positive influence of bacterial application on plant growth, with increased plant height (+ 18%), total chlorophylls (+ 42%), crop yields (+ 13 %) and bulbs dry matter (+ 3%) than the control. The differences between control and treated experimental conditions were also underlined in the bulb extracts in terms of total phenolic contents (+25%) and antioxidant activities (+20%). Soil fertility and microbial community structure and diversity were also positively affected by the bacterial inoculum. At harvest, the soil with the presence of the bacterial consortium showed an increase of total organic carbon, organic matter and available P and higher concentrations of nutrients than control. The ecological indexes calculated on the molecular profiles showed that community diversity was positively affected by the bacterial treatment. The present work allowed to remark the effective use of plant growth-promoting bacteria as valid fertilization strategy to improve yield in productive landscapes, whilst safeguarding soil biodiversity.

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Open field inoculation with PGPR as a strategy to manage fertilization of ancient Triticum genotypes

Cited by 47 publications

References 87 publications

Effects of Seed-Applied Biofertilizers on Rhizosphere Biodiversity and Growth of Common Wheat (Triticum aestivum L.) in the Field

Effects of Seed-Applied Biofertilizers on Rhizosphere Biodiversity and Growth of Common Wheat (Triticum aestivum L.) in the Field

Allium cepa L. Inoculation with a Consortium of Plant Growth-Promoting Bacteria: Effects on Plants, Soil, and the Autochthonous Microbial Community

<em>Allium cepa </em>L. <em>s</em>eed inoculation with a consortium of plant growth-promoting bacteria: effects on plant growth and development and soil fertility status and microbial community

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Open field inoculation with PGPR as a strategy to manage fertilization of ancient Triticum genotypes

Cited by 47 publications

References 87 publications

Effects of Seed-Applied Biofertilizers on Rhizosphere Biodiversity and Growth of Common Wheat (Triticum aestivum L.) in the Field

Effects of Seed-Applied Biofertilizers on Rhizosphere Biodiversity and Growth of Common Wheat (Triticum aestivum L.) in the Field

Allium cepa L. Inoculation with a Consortium of Plant Growth-Promoting Bacteria: Effects on Plants, Soil, and the Autochthonous Microbial Community

&lt;em&gt;Allium cepa &lt;/em&gt;L. &lt;em&gt;s&lt;/em&gt;eed inoculation with a consortium of plant growth-promoting bacteria: effects on plant growth and development and soil fertility status and microbial community

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<em>Allium cepa </em>L. <em>s</em>eed inoculation with a consortium of plant growth-promoting bacteria: effects on plant growth and development and soil fertility status and microbial community