Open-flow respirometry under field conditions: How does the airflow through the nest influence our results?

Abstract

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Reporting guidelines for terrestrial respirometry: Building openness, transparency of metabolic and evaporative water loss data

“…If a layer of mineral oil or similar is placed in the bottom of the chamber to prevent evaporation from excreta affecting EWL measurements, indicate the approximate depth of the layer. (McNab, 2006;Wu et al, 2015;Nowack et al, 2020) Incurrent air State the source of the incurrent air (e.g. outdoor air, gas cylinders).…”

“…Advances in technology have made the tools for measurement of energy and water balances more affordable and robust, resulting in a growing number of MR and EWL studies and also measurements taken outside of controlled conditions (Langer et al, 2018;Nowack et al, 2020;Reher et al, 2022). With the burgeoning field of biologging, it has increasingly providing novel insights into the energy and water budgeting of free-ranging individuals, applying knowledge of physiological principles in appropriate ecological settings (Chmura et al, 2018;Cooper et al, 2019).…”

Wake respirometry allows breath-by-breath assessment of ventilation and CO2 production in unrestrained animals

“…This is easiest for animals in prescribed hollows, such as bats in their roosting boxes, but may also work for animals outside. However, this has previously been done successfully in a number of studies using standard respirometry and will not necessarily alter the subject's metabolic rate (e.g., Nowack et al, 2020).…”