Open-source 3D printable frameless stereotaxic system for young and adult pigs

Malbert, C. H.

doi:10.1016/j.jneumeth.2021.109222

Cited by 1 publication

(1 citation statement)

References 28 publications

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“…Several neuroprotective treatments have been reported to be successful in permanent occlusion models in rodents ( 33 , 48 ) and recently in a permanent occlusion model in pigs ( 22 ), suggesting that there are opportunities for protection while in occlusion/ischemia. The recent publication of Porcine Neurological Imaging templates ( 49 , 50 ) and the open-source 3D printable stereotaxic systems ( 51 ) mean that there is also a clear advantage in adopting pigs as the translational model of choice to test new therapies.…”

Section: Discussionmentioning

confidence: 99%

Establishment of a reproducible and minimally invasive ischemic stroke model in swine

Castaño¹,

Melià-Sorolla²,

García-Serran³

et al. 2023

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The need for new advances in the management/treatment options for ischemic stroke patients requires that upcoming preclinical research uses animals with more human-like brain characteristics. The porcine brain is considered appropriate although the presence of the rete mirabile (RM) prevents direct catheterization of the intracranial arteries to produce focal cerebral ischemia. To develop a reproducible minimally invasive porcine stroke model, a catheter+guide was introduced through the femoral artery until reaching the left RM. Using the pressure cooker technique, Squid-12 embolization material was deposited to fill, overflow and occlude the left RM, the left internal carotid artery and left circle of Willis wing up to the origins of the middle cerebral 3 arteries (MCAs), thus mimicking the occlusion produced in the filament model in rodents.Longitudinal multimodal cerebral MR imaging was conducted to assess the brain damage and cerebral blood supply. The technique we describe here occluded up to the origins of the MCAs in 7 out of 8 swine, inducing early damage 90 min post-occlusion that later evolved to a large cerebral infarction, and producing no mortality during the intervention. This novel minimally invasive ischemic stroke model in swine produced reproducible infarcts and shows translational features common to human stroke.

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