Open-source recombinant monoclonal secondary nanobodies

Ewers, Helge

doi:10.1083/jcb.201802025

Cited by 2 publications

(2 citation statements)

References 9 publications

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“…Antibodies are ubiquitous reagents in research and diagnostics, allowing the detection of many molecules [ 12 ].…”

Section: Antibodies Versus Nanobodiesmentioning

confidence: 99%

“…Nanobodies or single-domain antibody fragments are found in sharks and camelids [ 11 ]. Despite their low molecular weight—they are approximately one-tenth the size of more common antibodies—they selectively bind to antigens with high affinity [ 12 ]. Compared to full-size antibodies, which may circulate for days, nanobodies have substantially faster pharmacokinetics due to their low molecular weight.…”

Section: Introductionmentioning

confidence: 99%

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Nanobodies as Diagnostic and Therapeutic Tools for Cardiovascular Diseases (CVDs)

et al. 2023

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The aim of this review is to summarize some of the most recent work in the field of cardiovascular disease (CVD) diagnosis and therapy, focusing mainly on the role of nanobodies in the development of non-invasive imaging methods, diagnostic devices, and advanced biotechnological therapy tools. In the context of the increased number of people suffering from CVDs due to a variety of factors such as sedentariness, poor nutrition, stress, and smoking, there is an urgent need for new and improved diagnostic and therapeutic methods. Nanobodies can be easily produced in prokaryotes, lower eukaryotes, and plant and mammalian cells, and offer great advantages. In the diagnosis domain, they are mainly used as labeled probes that bind to certain surface receptors or other target molecules and give important information on the severity and extent of atherosclerotic lesions, using imaging methods such as contrast-enhanced ultrasound molecular imaging (CEUMI), positron emission tomography (PET), single-photon emission computed tomography coupled with computed tomography (SPECT/CT), and PET/CT. As therapy tools, nanobodies have been used either for transporting drug-loaded vesicles to specific targets or as inhibitors for certain enzymes and receptors, demonstrated to be involved in various CVDs.

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“…Antibodies are ubiquitous reagents in research and diagnostics, allowing the detection of many molecules [ 12 ].…”

Section: Antibodies Versus Nanobodiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nanobodies as Diagnostic and Therapeutic Tools for Cardiovascular Diseases (CVDs)

et al. 2023

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Antibodies and microbial biomolecules as biotechnological tools for bacterial infections diagnosis

Luz

Caetano

Henrique

et al. 2020

New and Future Developments in Microbial Biotechnology and Bioengineering

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Open-source recombinant monoclonal secondary nanobodies

Abstract: Ewers outlines a toolbox of recombinant secondary nanobodies produced in E. coli, provided open source by the study from Pleiner, Bates, and Görlich.

Cited by 2 publications

References 9 publications

Nanobodies as Diagnostic and Therapeutic Tools for Cardiovascular Diseases (CVDs)

Nanobodies as Diagnostic and Therapeutic Tools for Cardiovascular Diseases (CVDs)

Antibodies and microbial biomolecules as biotechnological tools for bacterial infections diagnosis

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